蒙古马训练前后血糖相关基因的表达量分析

发布时间：2018-07-12 09:30

  本文选题：蒙古马 + 耐力训练　； 参考：《内蒙古农业大学》2015年硕士论文

【摘要】：马业是古老的产业,而我国是传统的养马国家,养马历史悠久。蒙古马是我国数量最多、分布最广的马,具有耐受性强、抗旱、抗寒、抗病等特性,在马的遗传资源上是一个极为宝贵的基因库。因此,对我国优秀地方品种蒙古马血糖相关基因进行研究,不仅可以进一步了解蒙古马耐力强的原因,为揭示蒙古马耐力强遗传特性提供理论依据,而且对地方品种马的改良及培育有着重要的意义。本研究以6匹遗传背景相似,并接受过为期四个月运动训练的蒙古马为研究对象。首先,通过运动训练对蒙古马血液生理生化指标进行测定,采用SAS(The system for Windows 9.0)软件对蒙古马训练前后血液生理生化指标的基础数据进行方差分析。结果表明,训练前后,血糖、肌酸激酶、尿素、血红蛋白、血红细胞、白细胞、淋巴细胞及血小板计数均呈现出显著变化。其中,训练前马匹血糖浓度为(4.77±0.42) mmol/L,训练后马匹血糖浓度为(5.83±0.31)mmol/L,高于训练前,前后差异显著(P0.05)。其次,分别对训练前后的蒙古马骨骼肌样品进行了转录组高通量测序分析,并对筛选到的差异基因进行GO功能富集,在term条目上,筛选到与血糖相关的LPHN3、LGALS3.IFIH1基因作为本研究的目的基因。采用实时荧光定量RT-qPCR方法检测蒙古马训练前后骨骼肌和血液中LPHN3、LGALS3、IFIH1基因的mRNA表达量的变化。结果表明,训练前后,LPHN3、LGALS3、IFIH1三个基因在蒙古马血液和骨骼肌中均有表达。其中,LPHN3基因在训练后蒙古马骨骼肌中极显著量高于训练前(P0.01),在训练前后,血液中的表达量差异不显著(P≥0.05)。LGALS3基因在训练后蒙古马骨骼肌和血液中的表达量均高于训练前,其中骨骼肌中的表达量差异显著(P0.05),血液中的表达差异极显著(P0.01)。IFIH1基因在训练后蒙古马血液中的表达量极显著高于训练前(P0.01),骨骼肌中表达量差异显著(P0.05)。
[Abstract]:Horse industry is an ancient industry, and our country is a traditional horse-raising country, horse-raising history is long. Mongolian horse is the most abundant and widely distributed horse in China, which has the characteristics of strong tolerance, drought resistance, cold resistance and disease resistance, and is an extremely valuable gene bank in horse genetic resources. Therefore, the study on the blood glucose related genes of Mongolian horse in China can not only further understand the reasons of Mongolian horse endurance, but also provide a theoretical basis for revealing the genetic characteristics of Mongolian horse endurance. Moreover, it is of great significance to the improvement and cultivation of local horses. In this study, 6 Mongolian horses with similar genetic background and four-month exercise training were studied. Firstly, the physiological and biochemical indexes of Mongolian horse blood were measured by exercise training, and the basic data of blood physiological and biochemical indexes before and after training were analyzed by using SAS (the system for Windows 9.0) software. The results showed that blood glucose, creatine kinase, urea, hemoglobin, red blood cell, white blood cell, lymphocyte and platelet count showed significant changes before and after training. The blood glucose concentration before training was (4.77 卤0.42) mmol / L, and that after training was (5.83 卤0.31) mmol / L, which was significantly higher than that before training (P0.05). Secondly, high-throughput transcriptional sequencing of Mongolian horse skeletal muscle samples before and after training was carried out, and the differentially screened genes were enriched with go function on the term entry. The LPHN3 LGALS3.IFIH1 gene associated with blood sugar was screened as the target gene in this study. Real-time quantitative RT-PCR was used to detect the mRNA expression of LPHN3, LGALS3, IFIH1 gene in skeletal muscle and blood of Mongolian horse before and after training. The results showed that LPHN3LGALS3 IFIH1 gene was expressed in Mongolian horse blood and skeletal muscle before and after training. The expression of LPHN3 gene in the skeletal muscle of Mongolian horse after training was significantly higher than that before training (P0.01), and there was no significant difference between before and after training (P 鈮，

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