新疆褐牛Leptin基因外显子Ⅱ E2JW多态性与体尺性状的相关性研究

发布时间：2018-07-16 12:55

【摘要】：为探讨新疆褐牛Leptin基因与体尺的相关性,以42头成年健康的新疆褐牛为研究对象,利用PCR-SSCP方法,检测Leptin基因外显子II E2JW多态性并与新疆褐牛体尺性状进行相关性分析。结果表明,Leptin基因E2JW具有三种基因型AA、AB和BB型,其基因型频率分别为0.1908、0.5238和0.2857。经测序发现:该位点T103C、C113T、C126G和T252A处存在突变。群体多态信息含量0.3727,属于中度多态、遗传杂合度0.4954,B等位基因是优势等位基因。与新疆褐牛相关性分析结果表明:AA基因型个体体高、十字部高均值显著高于BB基因型个体(P0.05)。BB基因型个体后腿围显著高于AB型(P0.05)。研究结果表明,新疆褐牛Leptin基因外显子II E2JW多态性与其体尺性状具有显著相关性,可作为新疆褐牛体尺性状选育的潜在分子标记位点。
[Abstract]:In order to study the correlation between leptin gene and body size of Xinjiang brown cattle, 42 adult healthy Xinjiang brown cattle were used to detect the polymorphism of leptin gene exon II E2JW by PCR-SSCP. The results showed that E2JW of Leptin gene had three genotypes, AA AB and BB, and the genotype frequencies were 0.1908, 0.5238 and 0.2857, respectively. After sequencing, it was found that there were mutations in C126G and T252A at the T103CU C113TX C126G and T252A locus. The polymorphic information content of population is 0.3727, which belongs to moderate polymorphism, and the genetic heterozygosity of 0.4954 B allele is the dominant allele. The results of correlation analysis with Xinjiang Brown cattle showed that the body height and cross height were significantly higher than those of BB genotype (P0.05). The hind leg circumference of BB genotype individuals was significantly higher than that of AB genotype individuals (P0.05). The results showed that the polymorphism of exon II E2JW of the leptin gene in Xinjiang brown cattle had a significant correlation with its body size traits, and could be used as a potential molecular marker for the breeding of body size traits of Xinjiang brown cattle.
【作者单位】：新疆农业大学动物科学学院;新疆畜牧科学院畜牧研究所;新疆农业大学动物医学学院;
【基金】：自治区科技重大专项课题(201230116-10) 国家肉牛(牦牛)产业技术体系(NYCYTX-38) 国家自然科学基金(31460647)
【分类号】：S823

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