肠炎沙门菌感染对济宁百日鸡空肠形态学的影响
发布时间：2018-07-17 02:35
【摘要】：为探讨肠炎沙门菌感染对空肠形态学的影响,研究选用济宁百日鸡阴性个体为试验材料,用肠炎沙门菌进行接种,分别在接种后第1、3、7、14、21和35天采取其空肠组织和盲肠内容物,采用石蜡切片和HE染色的方法对感染后7个不同时间点济宁百日鸡的空肠绒毛和隐窝进行组织形态学观察,同时检测7个时间点试验组和对照组盲肠内容物肠炎沙门菌的含量。结果表明:感染后7个时间点,对照组肠绒毛长度增加程度均大于试验组,且在接种后第1天,试验组绒毛长度显著低于对照组(P0.05)。除感染后第35天外,其余时间点试验组隐窝深度均大于对照组,接种后第3天与第7天,对照组与试验组隐窝深度差异显著(P0.05)。感染后第1、7、14、21天对照组绒毛长度与隐窝深度的比值(V/C)均显著大于试验组。研究结果初步揭示了肠炎沙门菌感染与鸡营养代谢的相关性。
[Abstract]:In order to investigate the effect of Salmonella enteritis infection on the morphology of jejunum, the study selected Jining 100 day chicken negative individuals as experimental materials, inoculated Salmonella enteritis with Salmonella enteritis, and took the jejunum tissue and cecum contents on day 1,3,7,14,21 and 35 days after inoculation, using paraffin section and HE staining method to 7 different time points after infection. The histomorphology of the jejunum villi and recess of the chicken was observed. At the same time, the content of Salmonella enteritis in the 7 time points and the control group was detected. The results showed that the length of the intestinal villi in the control group was higher than the test group at the 7 time points after the infection, and the length of the villi was significantly lower than that of the test group at first days after the inoculation. Except for thirty-fifth days after infection, the depth of recess in the test group at the rest of the time was greater than that of the control group, and the depth of the recess in the control group was significantly higher than the control group at third days and seventh days after the inoculation (P0.05). The ratio of the length of the villus to the depth of the recess (V/C) in the control group was significantly greater than that of the test group on day 1,7,14,21 after the infection. The results of the study were revealed preliminarily. Correlation between Salmonella enteritidis infection and chicken nutrition and metabolism.
【作者单位】： 山东农业大学动物科技学院;山东农业大学生命科学学院;山东省动物生物工程与疾病防治重点实验室;
【基金】：国家自然科学基金项目(31601980) 山东省现代农业产业技术体系项目(SDAIT-11-02) 山东省农业良种工程项目 山东省“双一流”奖补资金(SYL2017YSTD12)
【分类号】：S858.31
本文编号：2128636
[Abstract]:In order to investigate the effect of Salmonella enteritis infection on the morphology of jejunum, the study selected Jining 100 day chicken negative individuals as experimental materials, inoculated Salmonella enteritis with Salmonella enteritis, and took the jejunum tissue and cecum contents on day 1,3,7,14,21 and 35 days after inoculation, using paraffin section and HE staining method to 7 different time points after infection. The histomorphology of the jejunum villi and recess of the chicken was observed. At the same time, the content of Salmonella enteritis in the 7 time points and the control group was detected. The results showed that the length of the intestinal villi in the control group was higher than the test group at the 7 time points after the infection, and the length of the villi was significantly lower than that of the test group at first days after the inoculation. Except for thirty-fifth days after infection, the depth of recess in the test group at the rest of the time was greater than that of the control group, and the depth of the recess in the control group was significantly higher than the control group at third days and seventh days after the inoculation (P0.05). The ratio of the length of the villus to the depth of the recess (V/C) in the control group was significantly greater than that of the test group on day 1,7,14,21 after the infection. The results of the study were revealed preliminarily. Correlation between Salmonella enteritidis infection and chicken nutrition and metabolism.
【作者单位】： 山东农业大学动物科技学院;山东农业大学生命科学学院;山东省动物生物工程与疾病防治重点实验室;
【基金】：国家自然科学基金项目(31601980) 山东省现代农业产业技术体系项目(SDAIT-11-02) 山东省农业良种工程项目 山东省“双一流”奖补资金(SYL2017YSTD12)
【分类号】：S858.31
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1 ;选育家禽对肠炎沙门菌感染反应降低[J];中国家禽;2009年20期
2 朱国强;;国内禽病防控思路的分析与思考[J];中国家禽;2009年18期
，本文编号：2128636
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