大肠酶对猪饲料酶水解物能值的影响及与非淀粉多糖组分的关系

发布时间：2018-07-20 20:55

【摘要】：【目的】研究大肠酶对单胃动物仿生消化系统测试猪饲料原料的体外干物质消化率(DMD)和酶水解物能值(EHGE)的影响,并分析不同大肠酶条件下非淀粉多糖(NSP)组分与DMD和体外总能消化率(GED)的关系,为完善体外模拟胃-小肠-大肠三步消化方法提供参考依据。【方法】采用单因素完全随机设计,共设4个处理,在单胃动物仿生消化系统模拟饲料原料胃和小肠消化后,分别使用对照组(去离子水)、纤维素酶、Viscozyme酶和仿生酶(由纤维素酶、木聚糖酶、β-葡聚糖酶和果胶酶组成)模拟大肠阶段的消化。每个处理设5个重复,每个重复1根消化管,分别测定玉米、大豆粕、小麦麸、玉米DDGS、苜蓿草粉和大豆皮的DMD、GED和EHGE。并使用乙酸酐衍生化气相色谱法测定6种饲粮原料NSP含量和组分。分析饲料原料NSP组分与DMD及GED的相关关系。【结果】(1)在对照组中,玉米的DMD最高,达到了81.51%,相应的EHGE为15.39 MJ·kg~(-1),而大豆皮的DMD最低,只有10.60%,相应的EHGE只有2.42 MJ·kg~(-1)。(2)3种模拟大肠酶均显著提高了玉米、大豆粕和大豆皮的DMD(P0.01),提高了大豆粕和大豆皮的EHGE(P0.01)。但纤维素酶作用下苜蓿草粉、玉米DDGS的DMD和EHGE与对照组差异不显著,Viscozyme酶作用下小麦麸、玉米DDGS的DMD和EHGE与对照组差异不显著(P0.05)。仿生酶显著提高了6种饲料原料的DMD(P0.01),显著提高了除玉米DDGS外的其他5种饲料原料的EHGE(P0.01)。(3)不同的大肠酶对不同饲料原料体外消化率的提升程度不同。在6种原料中,纤维素酶对小麦麸的DMD和EHGE提升程度最高,分别达到了5.89%和1.03 MJ·kg~(-1),而只使大豆粕的DMD和EHGE提高了1.26%和0.36 MJ·kg~(-1);Viscozyme酶对大豆皮体外消化率的提升程度最高,分别使其DMD和EHGE提高6.01%和1.02 MJ·kg~(-1)。仿生酶对小麦麸的DMD和EHGE的提升程度最高,达到了6.59%和1.37 MJ·kg~(-1)。(4)6种饲料原料的可溶性非淀粉多糖(SNSP)的含量均低于不溶性非淀粉多糖(INSP),玉米的总非淀粉多糖(TNSP)含量最低(8.59%),大豆皮的TNSP含量最高(75.72%),各原料的NSP主要由阿拉伯糖、木糖、甘露糖和葡萄糖组成,但不同原料中4种单糖含量存在差异。(5)6种饲料原料的SNSP、INSP以及TNSP含量与DMD、GED均呈显著的负相关(P0.05)。仿生酶作用下DMD与TNSP含量的相关性(R~2=0.95,P0.01)高于纤维素酶(R~2=0.94,P0.01)和Viscozyme酶(R~2=0.93,P0.01)。同时,仿生酶作用下GED与TNSP含量的相关性(R~2=0.89,P0.01)也高于纤维素酶(R~2=0.86,P0.01)和Viscozyme酶(R~2=0.81,P0.01)。【结论】仿生酶在体外模拟猪大肠消化过程中,对饲料的消化作用优于纤维素酶和Viscozyme酶,可作为单胃动物仿生消化系统体外模拟猪消化中大肠阶段的模拟消化酶。
[Abstract]:[objective] to study the effects of colorectal enzymes on dry matter digestibility (DMD) and enzymatic hydrolysate energy (EHGE) of porcine feedstuff tested by bionic digestive system of monogastric animals. The relationship between non-starch polysaccharide (NSP) and DMD and total energy digestibility (GED) under different colonic enzyme conditions was analyzed. After digesting the stomach and the small intestine of monogastric bionic digestive system, the control group (deionized water), the cellulase Viscozyme enzyme and the bionic enzyme (by cellulase) were used respectively. Xylanase, 尾-glucanase and Pectinase) mimic the digestion of large intestine. Each treatment was divided into five replicates and one digestive tube per repeat. The DMD-GED and EHGE of corn, soybean meal, wheat bran, corn DDGS, alfalfa meal and soybean husk were determined respectively. The content and composition of NSP in 6 kinds of feedstock were determined by acetic anhydride derivatization gas chromatography. [results] (1) in the control group, the DMD of maize was the highest (81.51%), the corresponding EHGE was 15.39 MJ kg-1, and the DMD of soybean peel was the lowest. Only 10.60mg / kg and 2.42MJ kg-1). (_ 2, respectively, all three simulated colorectal enzymes significantly increased the DMD (P0.01) of corn, soybean meal and soybean husk, and the EHGE (P0.01) of soybean meal and soybean husk. However, there was no significant difference in DMD and EHGE between alfalfa meal and corn DDGS treated with cellulase and wheat bran treated with Viscozyme, and there was no significant difference in DMD and EHGE between maize DDGS and control group (P0.05). The bionic enzyme significantly increased the DMD (P0.01) of the six feed materials, and significantly increased the in vitro digestibility of the other five feed materials (EHGE (P0.01). (3). Among the six kinds of raw materials, cellulase had the highest enhancement of DMD and EHGE to wheat bran, which reached 5.89% and 1.03 MJ kg-1, respectively, but only increased the DMD and EHGE of soybean meal by 1.26% and 0.36 MJ kg-1, respectively, and increased the in vitro digestibility of soybean peel by 0.36 MJ kg-1 and 0.36 MJ kg-1, respectively. DMD and EHGE were increased by 6.01% and 1.02 MJ kg-1, respectively. The bionic enzyme had the highest enhancement of DMD and EHGE on wheat bran. The content of soluble non-starch polysaccharide (SNSP) of 6 feed raw materials reached 6.59% and 1.37 MJ kg-1). (_ 4 were lower than that of insoluble non-starch polysaccharide (INSP), the content of total non-starch polysaccharide (TNSP) of maize was the lowest (8.59%), the content of total non-starch polysaccharide of soybean peel was the highest (75.72%), and the content of total non-starch polysaccharide (TNSP) of each raw material was mainly arabinose. The composition of xylose, mannose and glucose, but the contents of four monosaccharides in different raw materials were different. (5) the contents of SNSPN INSP and TNSP of 6 kinds of feed raw materials were negatively correlated with DMD-GED (P0.05). The correlation between DMD and TNSP content under the action of bionic enzyme (RP0.01) was higher than that of cellulase (RP0.01) and Viscozyme enzyme (RP0.01). At the same time, the correlation between GED and TNSP content under the action of bionic enzyme (RP0.01) was higher than that of cellulase (RP0.01) and Viscozyme enzyme (RP0.01). [conclusion] Biomimetic enzyme was superior to cellulase and Viscozyme enzyme in digesting feed in the process of simulated pig large intestine digestion in vitro. It can be used as a mimic digestive enzyme of the bionic digestive system of monogastric animals in vitro to simulate the large intestine stage of pig digestion.
【作者单位】：中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室;
【基金】：国家科技支撑计划项目课题(2012BAD39B01) 中国农业科学院科技创新工程(ASTIP-IAS07) 国家重点实验室自主研究课题(2004DA125184G1104)
【分类号】：S816

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