川金果榄油膏治疗大鼠烫伤的药效学观察
发布时间：2018-07-20 20:25
【摘要】：烧伤是指由各种物理或化学因素,包括热液、蒸汽、火焰、电能、化学物质、放射线等所造成组织损伤的病症。针对频发的烧伤,中药治疗的研究也在不断深入。本课题主要观察川金果榄油膏对大鼠烫伤创面的保护作用,并进一步探讨其作用机理,为促进川金果榄油膏的临床应用及新剂型开发提供坚实的理论基础。主要实验内容及结果如下：1大鼠浅Ⅱ度烫伤模型的建立大鼠背部脱毛24h后,采用沸水加热的100g砝码分别作用于大鼠皮肤5s、10s、15s和20s,于烫伤24h后切取烫伤创面全层皮肤,制备病理切片,观察皮肤表皮层及真皮层的病理变化。得出结论：烫伤5s能建立稳定的大鼠浅II度烫伤模型,烫伤10s、15s、20s能建立不同程度的大鼠深II度烫伤模型。2川金果榄油膏对大鼠烫伤创面的保护作用SD大鼠20只,雌雄各半,随机分成5组,每组4只。伤后30min分别于各组烫伤部位给药,以均匀覆盖创面为宜,灭菌纱布包扎,每天给药1次,连续给药至创面完全愈合,模型组给予油膏基质处理。以上皮完全覆盖作为伤口完全愈合的依据。与模型组相比,高剂量组(P0.01)和中剂量组(P0.05)均能明显缩短创面的愈合时间。在伤后第7d,与模型组相比,高剂量组和中剂量组愈合率显著升高(P0.05),伤后14d亦延续这种趋势。从整个愈合过程中各组烫伤大鼠的病理组织学切片来看,川金果榄油膏能在早期减轻炎症细胞的浸润,加快创面纤维细胞和毛细血管生长,后期加快表皮覆盖,促进创面愈合。3川金果榄油膏促进大鼠烫伤创面愈合的作用机理SD大鼠84只,雌雄各半,其中正常组4只,其余大鼠随机均分为5组,每组16只。烫伤后,各组分别给予药物治疗,于伤后1d、3d、7d、14d各组随机选取4只大鼠,雌雄各半,尾静脉采血离心得血清,切取创面组织标本,保存于-80℃冰箱。通过对实验大鼠烫伤后1d-14d的部分生理指标的测定发现：伤后第3天,各组烫伤大鼠血清中IL-6含量上升趋势明显并达峰值,且均与正常组差异极显著(P0.01),间接反映造模成功。从创面组织中SOD和MDA含量可以看出,烫伤创面均在早期有一个不断加深的过程。而药物组创面羟脯氨酸含量均在伤后第3天达峰,早于模型组,且达峰浓度明显高于模型组。伤后第7天,与模型组相比,川金果榄油膏低剂量组创面组织含水量显著下降(P0.05),伤后第14天效果更加明显(P0.01)；川金果榄油膏高、中、低剂量组SOD活性均与模型组差异极显著(P0.01),各组MDA含量也均保持下降趋势,高剂量组下降幅度大,与模型组差异明显(P0.05)；各药物组创面组织中EGF含量的达峰时间均早于模型组,且在伤后第7天,川金果榄油膏高、中剂量组创面组织中EGF的含量显著高于模型组(P0.05)；各组烫伤大鼠血清中IL-6含量随时间延长而下降,其中,与模型组相比,川金果榄油膏高剂量组(P0.01)和中剂量组(P0.05)下降趋势更明显；各组血清中TGF-p的含量在伤后第7天达峰,且与模型组相比,高剂量组烫伤大鼠血清中TGF-p的含量显著升高(P0.01)。结论：1.运用加热5分钟的100g砝码致伤5s能建立稳定的大鼠浅II度烫伤模型。2.川金果榄油膏能较好地提高烫伤不同时间点创面的愈合率,缩短烫伤创面的愈合时间,对烫伤创面显示出较好的修复作用。3.川金果榄油膏促进烫伤创面愈合的作用机制可能涉及促进创面胶原生成,抑制脂质过氧化反应,促进细胞增殖和表皮爬行,控制部分炎症介质的过度合成和释放,促进烫伤大鼠EGF和TGF-p含量的升高。
[Abstract]:Burn is a disease of tissue damage caused by various physical or chemical factors, including hydrothermal, steam, flame, electric energy, chemical material, radiating line and so on. The study of Chinese medicine treatment for frequent burns is also in depth. This subject is mainly to observe the protective effect of the ointment on scald wounds in rats and further explore its effect. Mechanism to provide a solid theoretical basis for promoting the clinical application of Chuanjin olive oil ointment and the development of new dosage forms. The main experimental contents and results are as follows: 1 rats with shallow second degree scald model of 1 rats were established by boiling water heated 100g weights to 5S, 10s, 15s and 20s respectively, and scalded after 24h were cut to scald after scalding 24h. All layers of skin, pathological sections were prepared, and the pathological changes of skin epidermis and dermis were observed. Conclusion: scald 5S can establish a stable II degree scald model in rats, scald 10s, 15s, 20s can establish different degree of rat deep II scald model.2 Sichuan golden fruit olivan ointment to protect the scald wound of rats, SD rats 20, male and female. Half, randomly divided into 5 groups, each group was 4. After the injury, 30min was given to the scald parts of each group respectively. The wound was evenly covered with sterilized gauze, 1 times a day, the drug was given to the wound to complete healing. The model group was treated with ointment matrix. The complete cover was the basis of the complete healing of the wound. Compared with the model group, the high dose group (P0. 01) and medium dose group (P0.05) could significantly shorten the healing time of the wound. Compared with the model group, the healing rate of the high dose group and the medium dose group increased significantly after the injury (P0.05), and the 14d also continued the trend after the injury. From the pathological histological sections of the scalded rats during the whole healing process, the Sichuan golden ointment could reduce the early inflammation. The infiltration of the disease cells, quickening the growth of fibroblast and capillary in the wound, accelerating the skin cover in the later period, promoting the healing of the wound healing.3 and promoting the healing of scald wound in rats, 84 rats and male and male 4 rats were divided into 5 groups randomly, and the other rats were randomly divided into 5 groups, 16 rats in each group. After scald, the groups were given the drugs respectively. In the treatment, 4 rats were randomly selected from 1D, 3D, 7d, and 14d after injury. The blood samples from the tail vein were collected from the blood serum from the tail vein. The tissue specimens were cut from the wound tissue and stored at -80 fridge. Through the measurement of some physiological indexes of 1d-14d after scald in experimental rats, it was found that the increasing trend of IL-6 content in the serum of scalded rats was obvious and reached the third day after injury. The peak value was significantly different from that of the normal group (P0.01), which indirectly reflected the success of the mold making. From the content of SOD and MDA in the wound tissue, the burn wound had a continuous deepening process in the early stage. The content of hydroxyproline in the wound surface of the drug group were all in the third Tianda peak in the post injury, earlier than the model group, and the peak concentration was significantly higher than that in the model group. Seventh Compared with the model group, the water content of the wound tissue in the low dose group of the Sichuan golden olive oil ointment decreased significantly (P0.05), and the effect was more obvious (P0.01) fourteenth days after injury. The activity of SOD in the medium and low dose group was very significant (P0.01), and the content of MDA in each group also kept declining, and the decrease of the high dose group was large, and the model was large. The peak time of EGF content in the wound tissue of each drug group was earlier than that in the model group, and the content of EGF in the wound tissue in the middle dose group was higher than that in the model group (P0.05) in the middle dose group seventh days after the injury, and the IL-6 content in the serum of the scalded rats decreased with the time prolonging, which was compared with the model group. The decreasing trend of the high dose group (P0.01) and the medium dose group (P0.05) was more obvious, and the content of TGF-p in the serum of each group was seventh Tianda peak after injury, and compared with the model group, the content of TGF-p in the serum of scalded rats increased significantly (P0.01). Conclusion: 1. using the 100g weight of 5 minutes heating for 5 minutes to induce the injury of 5S can establish a stable shallow rat. The II degree scald model.2. Sichuan golden ointment ointment can improve the healing rate of burn wound at different time points, shorten the healing time of scald wound, and show a better repair effect on scald wound. The mechanism of.3. Kaga ruga ointment to promote the healing of scald wound may involve the formation of collagen in the wound surface and the inhibition of lipid peroxidation. It can promote cell proliferation and epidermal crawling, control excessive synthesis and release of some inflammatory mediators, and promote the increase of EGF and TGF-p contents in scalded rats.
【学位授予单位】：四川农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S853.7
本文编号：2134676
[Abstract]:Burn is a disease of tissue damage caused by various physical or chemical factors, including hydrothermal, steam, flame, electric energy, chemical material, radiating line and so on. The study of Chinese medicine treatment for frequent burns is also in depth. This subject is mainly to observe the protective effect of the ointment on scald wounds in rats and further explore its effect. Mechanism to provide a solid theoretical basis for promoting the clinical application of Chuanjin olive oil ointment and the development of new dosage forms. The main experimental contents and results are as follows: 1 rats with shallow second degree scald model of 1 rats were established by boiling water heated 100g weights to 5S, 10s, 15s and 20s respectively, and scalded after 24h were cut to scald after scalding 24h. All layers of skin, pathological sections were prepared, and the pathological changes of skin epidermis and dermis were observed. Conclusion: scald 5S can establish a stable II degree scald model in rats, scald 10s, 15s, 20s can establish different degree of rat deep II scald model.2 Sichuan golden fruit olivan ointment to protect the scald wound of rats, SD rats 20, male and female. Half, randomly divided into 5 groups, each group was 4. After the injury, 30min was given to the scald parts of each group respectively. The wound was evenly covered with sterilized gauze, 1 times a day, the drug was given to the wound to complete healing. The model group was treated with ointment matrix. The complete cover was the basis of the complete healing of the wound. Compared with the model group, the high dose group (P0. 01) and medium dose group (P0.05) could significantly shorten the healing time of the wound. Compared with the model group, the healing rate of the high dose group and the medium dose group increased significantly after the injury (P0.05), and the 14d also continued the trend after the injury. From the pathological histological sections of the scalded rats during the whole healing process, the Sichuan golden ointment could reduce the early inflammation. The infiltration of the disease cells, quickening the growth of fibroblast and capillary in the wound, accelerating the skin cover in the later period, promoting the healing of the wound healing.3 and promoting the healing of scald wound in rats, 84 rats and male and male 4 rats were divided into 5 groups randomly, and the other rats were randomly divided into 5 groups, 16 rats in each group. After scald, the groups were given the drugs respectively. In the treatment, 4 rats were randomly selected from 1D, 3D, 7d, and 14d after injury. The blood samples from the tail vein were collected from the blood serum from the tail vein. The tissue specimens were cut from the wound tissue and stored at -80 fridge. Through the measurement of some physiological indexes of 1d-14d after scald in experimental rats, it was found that the increasing trend of IL-6 content in the serum of scalded rats was obvious and reached the third day after injury. The peak value was significantly different from that of the normal group (P0.01), which indirectly reflected the success of the mold making. From the content of SOD and MDA in the wound tissue, the burn wound had a continuous deepening process in the early stage. The content of hydroxyproline in the wound surface of the drug group were all in the third Tianda peak in the post injury, earlier than the model group, and the peak concentration was significantly higher than that in the model group. Seventh Compared with the model group, the water content of the wound tissue in the low dose group of the Sichuan golden olive oil ointment decreased significantly (P0.05), and the effect was more obvious (P0.01) fourteenth days after injury. The activity of SOD in the medium and low dose group was very significant (P0.01), and the content of MDA in each group also kept declining, and the decrease of the high dose group was large, and the model was large. The peak time of EGF content in the wound tissue of each drug group was earlier than that in the model group, and the content of EGF in the wound tissue in the middle dose group was higher than that in the model group (P0.05) in the middle dose group seventh days after the injury, and the IL-6 content in the serum of the scalded rats decreased with the time prolonging, which was compared with the model group. The decreasing trend of the high dose group (P0.01) and the medium dose group (P0.05) was more obvious, and the content of TGF-p in the serum of each group was seventh Tianda peak after injury, and compared with the model group, the content of TGF-p in the serum of scalded rats increased significantly (P0.01). Conclusion: 1. using the 100g weight of 5 minutes heating for 5 minutes to induce the injury of 5S can establish a stable shallow rat. The II degree scald model.2. Sichuan golden ointment ointment can improve the healing rate of burn wound at different time points, shorten the healing time of scald wound, and show a better repair effect on scald wound. The mechanism of.3. Kaga ruga ointment to promote the healing of scald wound may involve the formation of collagen in the wound surface and the inhibition of lipid peroxidation. It can promote cell proliferation and epidermal crawling, control excessive synthesis and release of some inflammatory mediators, and promote the increase of EGF and TGF-p contents in scalded rats.
【学位授予单位】：四川农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S853.7
【相似文献】
相关期刊论文 前3条
1 冯世鑫;马小军;闫志刚;潘丽梅;黄曦;;光照强度对金果榄光合特性和生长品质的影响[J];激光生物学报;2011年04期
2 李福杏;;金果榄在兽医临床上的应用研究[J];四川畜牧兽医;2009年11期
3 ;[J];;年期
相关会议论文 前3条
1 黄良永;郑江萍;;金果榄不同提取方法比较研究[A];湖北省药学会第十一届会员代表大会暨2007年学术年会论文汇编[C];2007年
2 涂自良;杨光义;陈吉炎;朱海涛;肖淼生;;金果榄水提液和醇提液指标成分的相关性比较[A];湖北省药学会第十一届会员代表大会暨2007年学术年会论文汇编[C];2007年
3 董少华;杨光义;杨标;冯正锋;孟盼;周圆;;金果榄总生物碱提取工艺研究[A];《医药导报》第八届编委会成立大会暨2009年度全国医药学术交流会和临床药学与药学服务研究进展培训班论文集[C];2009年
相关博士学位论文 前1条
1 杨光义;武当山金果榄药材资源开发利用研究[D];广州中医药大学;2009年
相关硕士学位论文 前3条
1 陈黎;湖北金果榄物质基础研究[D];湖北中医学院;2008年
2 王玉文;金果榄及双丹方毛细管电泳分析研究[D];浙江大学;2006年
3 张睿;川金果榄油膏治疗大鼠烫伤的药效学观察[D];四川农业大学;2015年
，本文编号：2134676
本文链接：https://www.wllwen.com/yixuelunwen/dongwuyixue/2134676.html
