发酵果蔬液体饲料最佳工艺的研究及对断奶仔猪生长性能的影响
发布时间:2018-07-21 20:30
【摘要】:本研究旨在研究接种益生菌发酵果蔬液体饲料的最佳工艺组合,在最佳发酵工艺条件下发酵果蔬液体饲料后用于断奶仔猪饲养对比。研究果蔬发酵液体饲料相对于颗粒料、常规液体发酵饲料及添加苹果渣的液体发酵饲料,对仔猪生长性能、血液常规、肠道菌群等指标的影响。具体研究结果如下:试验一:单菌、混菌发酵果蔬液体饲料最佳工艺的研究本试验通过对果蔬液体饲料接种酵母菌、枯草芽孢杆菌、植物乳杆菌单菌发酵的单因素试验和正交试验,探讨发酵时间、发酵温度、菌种接种量、饲料pH值等参数对果蔬液体饲料营养价值的影响,其研究结果如下。酵母菌最佳优化条件为好氧条件下,发酵时间60h、温度30℃、pH 6.0、接种量3%。在最佳优化条件下经酵母菌发酵果蔬液体饲料,粗蛋白含量上升至19.41%,粗纤维含量减小至2.09%,乳酸含量增加约7.38倍,钙、磷含量也有不同程度的增加。枯草芽孢杆菌最佳优化条件为好氧条件下,温度30℃、发酵时间60h、pH 5.0、接种量5%。在最佳优化条件下经枯草芽孢杆菌发酵后,粗蛋白含量上升至19.71%,粗纤维含量减小至2.31%,乳酸含量达到16.98g/L,钙、磷含量同样也有不同程度的增加。植物乳杆菌最佳优化条件为厌氧条件下,发酵时间60h、温度37℃、接种量3%、pH 6.0。在最佳优化条件下经植物乳杆菌发酵后,粗蛋白含量上升至19.38%,粗纤维含量减小至2.37%,乳酸含量达到62.11 g/L,钙,磷含量上升不明显。将上述三种菌按最佳发酵条件混菌异步发酵后,饲料营养价值比单一菌种发酵的营养价值有所提高,干物质含量中粗蛋白含量比发酵前上升9.2%,达到19.88%;粗纤维含量降低至2.01%;乳酸、生物量、Ca、P也有一定的提升,分别提高至61.88g/L、162.73×106 cfu/ml、0.99%和0.91%。试验二:果蔬发酵液体饲料对断奶仔猪生长性能、血液常规、肠道菌群等指标的影响随机挑选同时28日龄断奶的健康仔猪200头,采用随机分组方式分成4个处理组,分别为A组饲喂颗粒料基础日粮、B组饲喂液体饲料、C组饲喂含30g/kg苹果渣的液体饲料、D组饲喂含30g/kg的果蔬混菌发酵液体饲料。每个组5个重复,每个重复10头猪。试验预试期7天,正试期为30天。饲养试验结束后,从每个重复中选取体形、体重适中的仔猪各一只,收集小肠各段内容物用于肠道微生物菌群的测定。试验结果表明,在平均日增重方面,A、B、C、D组之间两两差异显著(P0.05),其中D组即果蔬混菌发酵液体饲料组比A组提高19.01%,比B组提高13.91%,比C组提高10.59%且均差异显著(P0.05)。在料重比方面,B、C两组比A组分别降低1.71%和0.57%均差异显著(P0.05);D组比A组降低10.29%(P0.05,差异显著)。混菌发酵果蔬液体饲料可有显著提高胃肠中乳酸菌含量,显著降低大肠杆菌含量,对断奶仔猪胃肠内容物pH值无显著影响。果蔬混菌发酵液体饲料能够提高断奶仔猪血液生化指标,降低超氧化物歧化酶和血清尿素氮含量,对机体免疫功能没有负面影响。
[Abstract]:The purpose of this study was to study the optimum technology combination of fermentation liquid feed of fruits and vegetables with probiotics, and to ferment the liquid feed of fruit and vegetable under the best fermentation conditions, and then use it for weaning piglets. The effects of fermented liquid feed on growth performance, blood routine and intestinal microflora of piglets were studied. The results are as follows: experiment 1: the optimum technology of fermentation of liquid feed for fruit and vegetable by single bacteria and mixed bacteria; this experiment inoculated the liquid feed of fruit and vegetable with yeasts, Bacillus subtilis, Bacillus subtilis and Bacillus subtilis. The effects of fermentation time, fermentation temperature, inoculation amount and feed pH on nutritional value of liquid feed for fruits and vegetables were studied by single factor test and orthogonal experiment of Lactobacillus plantarum fermentation. The results are as follows. The optimum conditions for yeast fermentation were aerobic, fermentation time 60 h, temperature 30 鈩,
本文编号:2136769
[Abstract]:The purpose of this study was to study the optimum technology combination of fermentation liquid feed of fruits and vegetables with probiotics, and to ferment the liquid feed of fruit and vegetable under the best fermentation conditions, and then use it for weaning piglets. The effects of fermented liquid feed on growth performance, blood routine and intestinal microflora of piglets were studied. The results are as follows: experiment 1: the optimum technology of fermentation of liquid feed for fruit and vegetable by single bacteria and mixed bacteria; this experiment inoculated the liquid feed of fruit and vegetable with yeasts, Bacillus subtilis, Bacillus subtilis and Bacillus subtilis. The effects of fermentation time, fermentation temperature, inoculation amount and feed pH on nutritional value of liquid feed for fruits and vegetables were studied by single factor test and orthogonal experiment of Lactobacillus plantarum fermentation. The results are as follows. The optimum conditions for yeast fermentation were aerobic, fermentation time 60 h, temperature 30 鈩,
本文编号:2136769
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