内蒙古荒漠草原土壤可培养真菌的群落结构和空间分布分析

发布时间：2018-07-22 17:20

【摘要】：为了揭示荒漠草原土壤可培养真菌的群落结构和空间分布,以实现利用微生物对环境变化的敏感性对退化草地提前预测和有效管理,采用稀释平板涂布法和18SrRNA分子鉴定技术对内蒙古荒漠草原土壤可培养真菌的群落结构、多样性、空间分布及其与土壤环境因子的相关性进行分析。结果表明:可培养真菌在0~30cm土层的数量为26.65×104~90.69×104 CFU·g-1,各土层的真菌分布次序为:0~10cm10~20cm20~30cm。分离到的真菌分别属于14个属,其中青霉菌属是优势菌属,占0~30cm可培养真菌总数的48.76%,其次是曲霉属,占可培养真菌总数的10%。在0~10cm土层,可培养真菌的物种丰富度和Shannon-Wiener指数(H')最高。可培养真菌的数量与含水量、速效钾和铵态氮呈显著正相关关系(P0.05);与有机质含量呈极显著正相关关系(P0.01),与pH值呈显著负相关关系(P0.05);与硝态氮和速效磷无显著相关性。
[Abstract]:In order to reveal the community structure and spatial distribution of culturable fungi in desert steppe soil in order to make use of the sensitivity of microorganisms to environmental change to predict and effectively manage degraded grassland in advance. The community structure, diversity, spatial distribution and correlation with soil environmental factors of culturable fungi in desert steppe of Inner Mongolia were analyzed by dilution plate coating method and 18s rRNA molecular identification technique. The results showed that the number of culturable fungi in 0~30cm soil layer was 26.65 脳 10 ~ 4 ~ 90.69 脳 10 ~ 4 CFU / g ~ (-1), and the distribution order of fungi in each soil layer was: 10 ~ (10) cm ~ (10) ~ (10) ~ (10) ~ (20) cm ~ (20) ~ (20) ~ (30) cm ~ (-1). The isolated fungi belong to 14 genera, of which Penicillium is the dominant genus, accounting for 48.76% of the total number of culturable fungi in 0~30cm, followed by Aspergillus, accounting for 10% of the total number of culturable fungi. The species richness and Shannon-Wiener index (H') of culturable fungi were highest in 0~10cm soil layer. The number of culturable fungi was positively correlated with water content, available potassium and ammonium nitrogen (P0.05), the content of organic matter was significantly positively correlated (P0.01), and negatively correlated with pH value (P0.05), and had no significant correlation with nitrate and available phosphorus.
【作者单位】：天津师范大学天津市水资源与水环境重点实验室;天津师范大学生命科学学院;内蒙古农业大学草原与资源环境学院;
【基金】：国家自然科学基金(31500365,31100330,31270502) 天津市自然科学基金(12JCYBJC19700) 天津市科技支撑基金项目(15ZCZDSF00410)资助
【分类号】：S812.2

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