两种毛色牦牛皮肤黑色素组织学分析

发布时间：2018-07-25 14:01

【摘要】：【目的】观察两种不同毛色的牦牛皮肤中黑色素分布。【方法】采用冰冻切片和石蜡切片技术,用甲苯胺蓝和HE染色进行组织学分析。【结果】两种切片方法均能展现毛囊结构、皮肤组织分层结构。甲苯胺蓝染色:石蜡切片比冰冻切片着色较浅,易观察到黑色素的分布;冰冻切片通过显微镜观察发现牦牛毛囊由毛鞘、毛乳头和毛球外围的结缔组织形成。HE染色:冰冻切片着色较深,皮肤结构层次分明;石蜡切片着色均匀,细胞分布明显。【结论】天祝白牦牛表皮的基底层和毛囊周围发现分布着少量的黑色素。大通牦牛的毛囊和表皮中均发现有大量黑色素的存在。大通牦牛毛囊中与毛发相连毛乳头附近的黑色素含量比毛囊周围其他部位含量高,皮肤表皮基底层相对毛囊黑色素含量少。大通牦牛毛囊周围黑色素比天祝白牦牛密集,且更为明显。大通牦牛的毛囊群比天祝牦牛毛囊群密集,毛囊的直径比天祝牦牛的长,毛发较粗。石蜡切片的HE染色是比较好的方法。
[Abstract]:[objective] to observe the distribution of melanin in yak skin with two different coat colors. [methods] Frozen and paraffin sections were used. The histological analysis was performed by toluidine blue and HE staining. [results] both methods could show the structure of hair follicle and the stratified structure of skin tissue. Toluidine blue staining: paraffin sections were lighter colored than frozen sections, and the distribution of melanin was easy to be observed. Through microscopic observation, frozen sections found that yak hair follicles were sheathed. The connective tissue around the hair nipple and hair bulb was stained with HE: the frozen sections were stained deeply, the skin structure was clear, the paraffin sections were stained evenly, [conclusion] A small amount of melanin was found in the basal layer and around the hair follicles of Tianzhu white yak epidermis. A large amount of melanin was found in hair follicles and epidermis of Datong yak. The content of melanin in hair follicle of Datong yak was higher than that in other parts around hair follicle, and the content of melanin in basal layer of skin epidermis was less than that in hair follicle. The melanin around hair follicle of Datong yak is denser and more obvious than that of Tianzhu white yak. The hair follicle of Datong yak is denser than that of Tianzhu yak, the diameter of hair follicle is longer than that of Tianzhu yak, and the hair is thicker than that of Tianzhu yak. The HE staining of paraffin sections is a better method.
【作者单位】：中国农业科学院兰州畜牧与兽药研究所甘肃省牦牛繁育重点实验室;甘肃农业大学动物科学与技术学院;
【基金】：基金项目:牦牛资源与育种创新工程(CAAS-ASTIP-2014-LIHPS-01) 现代农业(肉牛牦牛)产业技术体系(CARS-38)
【分类号】：S823.85

【参考文献】