加系SPF大白和长白猪群体遗传学分析与SLA单倍型的初步筛选

发布时间：2018-07-25 20:12

【摘要】：本试验首先采用19对微卫星引物对中国农业科学院哈尔滨兽医研究所从加拿大引进100头无猪瘟、猪繁殖与呼吸综合征、猪传染性胃肠炎、猪流感等病原体的SPF猪进行了群体遗传学检测。其次,又对大白猪和长白猪的二元杂交后代大长和长大猪进行了群体遗传学检测。最后,再利用低分辨率的PCR-SSP技术对大白猪和长白猪个体进行SLA基因单倍型初步筛选。总体研究探讨了SPF商品猪作为实验动物模型的可行性,研究结果如下:1.加系SPF大白猪和长白猪的19个微卫星位点均表现为高度多态,其中S0155、S0143、S0178、Sw857和Sw936位点等位基因在大白和长白两猪群中的分布差异显著(P0.05),可作为该品种品种鉴定的候选位点。2.加系SPF大白猪和长白猪的平均杂合度(He)分别为0.6066和0.5877(介于0.5~0.7之间)。观测杂合度(Ho)和期望杂合度(He)分别为0.5875、0.5877和0.6156、0.6066,无差异(P0.05),符合封闭群遗传学指标要求,可作为实验动物模型应用于猪病研究和疫苗制备。3.其后代二元杂交长大群体的平均杂合度为0.7322,大于0.7,不符合封闭群遗传学指标。除IGF1和S0218外,大长和长大群体的其他17个位点的基因型分化(Fit)均为负值,杂合度过剩,需要进一步选育。4.遗传结构和分化分析表明,大白猪和长白猪两个群体内分化较小,遗传结构稳定。5.2922、2992、2956、2952、3033、3065、5131、5132样品分选出8个SLA-1 BLANK(新的)等位基因。
[Abstract]:In this experiment, 19 pairs of microsatellite primers were used to introduce 100 pigs without swine fever, porcine reproductive and respiratory syndrome, and infectious gastroenteritis from Canada to Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences. SPF pigs with swine flu and other pathogens were tested for population genetics. Secondly, population genetic analysis was carried out on the two hybrid progenies of Landrace and Landrace. Finally, low resolution PCR-SSP technique was used to screen the haplotypes of SLA gene in large white pigs and Landrace pigs. The overall study explored the feasibility of SPF commercial pigs as an experimental animal model, the results of which are as follows: 1. 1. The 19 microsatellite loci of SPF and Landrace were highly polymorphic, and the alleles of S0155, S0145, S01788, Sw857 and Sw936 were significantly different between Landrace and Landrace (P0.05), which could be used as candidate loci for identification of this breed. The average heterozygosity (He) of SPF white pigs and Landrace pigs were 0.6066 and 0.5877, respectively. The observed heterozygosity (Ho) and expected heterozygosity (He) were 0.5875 (0.5877) and 0.6156 (0.6066), respectively. There was no difference (P0.05), which could be used as an experimental animal model for swine disease research and vaccine preparation. The average heterozygosity was 0.7322, larger than 0.7, which did not accord with the genetic index of closed population. With the exception of IGF1 and S0218, the genotypic differentiation (Fit) of the other 17 loci were all negative, and the heterozygosity was excess, which required further breeding. 4. The genetic structure and differentiation analysis showed that the differentiation between large white pig and Landrace pig was relatively small, and the genetic structure was stable. The genetic structure was stable.
【学位授予单位】：甘肃农业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S828

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