硒增强二十二碳六烯酸在脂多糖诱导巨噬细胞炎性反应中的抗炎作用

发布时间：2018-07-26 08:36

【摘要】：为了探究硒是否影响二十二碳六烯酸(DHA)在脂多糖(LPS)诱导巨噬细胞炎性反应中发挥的抗炎作用。小鼠巨噬细胞系RAW264.7细胞分别经10μg/m L DHA、10μg/m L DHA+0.05μmol/L亚硒酸钠、1μg/m L LPS、10μg/m L DHA+1μg/m L LPS、10μg/m L DHA+1μg/m L LPS+0.05μmol/L亚硒酸钠诱导24 h,同时设置无添加的正常组。采用半定量反转录PCR检测细胞肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、白细胞介素6(IL-6)和白细胞介素10(IL-10)mRNA表达量,酶联免疫吸附测定(ELISA)法测定培养液上清液中TNF-α、IL-1β、IL-6和IL-10含量。结果显示,添加亚硒酸钠(0.05μmol/L)不仅显著或极显著增强了DHA(10μg/m L)对LPS(1μg/m L)诱导RAW 264.7细胞中促炎细胞因子TNF-αmRNA表达(P0.05)和IL-1β生成(P0.01)的抑制作用,还极显著增强了DHA(10μg/m L)对LPS(1μg/m L)诱导RAW264.7细胞中抗炎细胞因子IL-10 mRNA表达的促进作用(P0.01)。由此提示,硒可增强DHA在LPS诱导巨噬细胞炎症反应中的抗炎作用。
[Abstract]:To investigate whether selenium affects the anti-inflammatory effect of 22 carbohexaenoic acid (DHA) on the inflammatory response of macrophages induced by lipopolysaccharide (LPS). Mouse macrophage cell line RAW264.7 was induced by 10 渭 g / mL DHAA 10 渭 g / mL DHA 0.05 渭 mol/L sodium selenite 1 渭 g / mL LPS1 渭 g / mL DHA 1 渭 g / mL DHA 10 渭 g / mL DHA 1 渭 g / mL LPS 0.05 渭 mol/L sodium selenite for 24 h, respectively. The expressions of tumor necrosis factor- 伪 (TNF- 伪), interleukin-1 尾 (IL-1 尾), interleukin-6 (IL-6) and interleukin-10 (IL-10) mRNA were detected by semi-quantitative reverse transcription PCR. The contents of TNF- 伪, IL-1 尾 and IL-10 in the supernatant were measured by enzyme-linked immunosorbent assay (Elisa). The results showed that the addition of sodium selenite (0. 05 渭 mol/L) not only significantly or extremely enhanced the inhibitory effect of DHA (10 渭 g / mL) on the expression of TNF- 伪 mRNA and the production of IL-1 尾 (P0. 01) in RAW 264.7 cells induced by LPS (1 渭 g / mL). The effect of DHA (10 渭 g / mL) on the expression of anti-inflammatory cytokine IL-10 mRNA in RAW264.7 cells induced by LPS (1 渭 g / mL) was also significantly enhanced (P0.01). The results suggest that selenium can enhance the anti-inflammatory effect of DHA in LPS-induced macrophage inflammation.
【作者单位】：四川农业大学动物医学院;
【基金】：四川农业大学学科建设双支计划(3572070) 四川省教育厅重点项目(15ZA0024)
【分类号】：S816

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