奶牛子宫内膜炎抗菌蒙药复方的筛选及其抗菌作用的研究
发布时间：2018-07-27 12:47
【摘要】：奶牛子宫内膜炎是引发奶牛不孕症的主要原因之一,因其较高的发病率,使得我国的奶牛产业遭受重大的经济损失。奶牛子宫内膜炎最为常用的治疗方法是抗生素和其他化学药物子宫内灌注和子宫内冲洗。但是,由滥用抗生素和其他化学药物引起的抗生素残留和耐药菌株产生数量及速度的不断增加,需求一种能够治疗奶牛子宫内膜炎的绿色强效经济药物成为一个亟待解决的问题。蒙药具有抗菌、抗炎免疫和寒热调理作用,通过现代的研究方法,希望能从中筛选出有效治疗奶牛子宫内膜炎的药物组方。主要研究内容及结果如下：1、以常规方法从奶牛子宫内膜炎分泌物中分离纯化主要致病菌,经分离纯化以及生化鉴定所得主要致病菌为大肠杆菌和金黄色葡萄球菌,其中大肠杆菌64株,金黄色葡萄球菌17株。2、以玻片凝集法对分离所得的64株大肠杆菌进行抗“O”血清型分析,鉴定结果为33株11种血清型,即O86、O1、O126、O111、O29、O28、O8、O91、O2、O26、O25。其中以0s6鉴定率最高为14%,为优势血清型,其余菌株有4株出现自凝集现象,27株未能检测出。3、对大黄、连翘、诃子等26味蒙药经体外抑菌试验结合正交方法,筛选蒙药复方并对其进行剂量优化,得到4组蒙药复方,分别为复方Ⅰ：大黄70g、丹参50g；复方Ⅱ：黄芩70g、大黄50g；复方Ⅲ：大黄、诃子各50g,连翘30g；复方Ⅴ：黄芩、大黄、诃子各70g。4、以昆明系小鼠为试验动物,测定筛选出的复方对小鼠(攻毒菌量为80%混合菌致死量)的保护率。结果为复方Ⅰ、Ⅱ、Ⅲ、Ⅴ对攻毒小鼠的保护率分别为40%、40%、60%和50%,与环丙沙星组对比,其中复方Ⅴ与环丙沙星组效果相当,复方Ⅲ高于环丙沙星组。5、以体外抑菌方法测定蒙药复方的抗菌谱,结果为无乳链球菌、鼠伤寒沙门氏菌、肠出血性大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌、蜡样芽孢杆菌对4组复方均为高敏感或极敏感,其中复方Ⅱ的抑菌圈直径显著高于其他复方(P0.05)。6、以酶标比浊法测定复方药物对两种致病菌生长曲线的影响,微生物粘附法测定复方药物对菌体细胞表面疏水性的影响,邻硝基苯-β-D-半乳吡喃糖苷(ONPG)法测定复方药物对菌体细胞膜渗透性的影响,测定碱性磷酸酶(APK)活性法测定复方药物对菌体细胞壁的影响。所得结果为4组蒙药复方均能很好地影响致病菌的生长曲线,且都能够从不同程度上影响菌体细胞表面的疏水性以及其细胞膜的渗透性,对菌体细胞壁也有不同程度的损伤。
[Abstract]:Endometritis is one of the main causes of infertility in dairy cows. Because of its high incidence, the dairy industry in China has suffered great economic losses. The most commonly used treatment for endometritis in cows is intrauterine perfusion and irrigation with antibiotics and other chemicals. However, the number and rate of production of antibiotic residues and resistant strains resulting from the abuse of antibiotics and other chemical drugs is increasing, The demand for a green economic drug for the treatment of dairy endometritis has become an urgent problem. Mongolian medicine has antimicrobial, anti-inflammatory, immune and cold-heat conditioning effects. Through modern research methods, it is hoped that it can be used to screen effective prescriptions for the treatment of endometritis in dairy cows. The main research contents and results are as follows: 1. The main pathogenic bacteria were isolated and purified from the secretion of endometritis of dairy cows by routine method. The main pathogenic bacteria were Escherichia coli and Staphylococcus aureus after isolation, purification and biochemical identification. Among them, 64 strains of Escherichia coli and 17 strains of Staphylococcus aureus were analyzed by slide agglutination method. The results showed that 33 strains of 11 serotypes, namely O86O1O126O111O29O28O28O91O2O26O25. Among them, the 0s6 identification rate was the highest, and the dominant serotype was 14. The other 4 strains showed self-agglutination phenomenon. 27 strains could not be detected. 26 Mongolian drugs, such as rhubarb, Forsythia suspensa and Fructus chebula, were tested by in vitro bacteriostasis test and orthogonal method. The Mongolian medicine compound was selected and optimized in dosage. The four groups were compound 鈪，
本文编号：2147879
[Abstract]:Endometritis is one of the main causes of infertility in dairy cows. Because of its high incidence, the dairy industry in China has suffered great economic losses. The most commonly used treatment for endometritis in cows is intrauterine perfusion and irrigation with antibiotics and other chemicals. However, the number and rate of production of antibiotic residues and resistant strains resulting from the abuse of antibiotics and other chemical drugs is increasing, The demand for a green economic drug for the treatment of dairy endometritis has become an urgent problem. Mongolian medicine has antimicrobial, anti-inflammatory, immune and cold-heat conditioning effects. Through modern research methods, it is hoped that it can be used to screen effective prescriptions for the treatment of endometritis in dairy cows. The main research contents and results are as follows: 1. The main pathogenic bacteria were isolated and purified from the secretion of endometritis of dairy cows by routine method. The main pathogenic bacteria were Escherichia coli and Staphylococcus aureus after isolation, purification and biochemical identification. Among them, 64 strains of Escherichia coli and 17 strains of Staphylococcus aureus were analyzed by slide agglutination method. The results showed that 33 strains of 11 serotypes, namely O86O1O126O111O29O28O28O91O2O26O25. Among them, the 0s6 identification rate was the highest, and the dominant serotype was 14. The other 4 strains showed self-agglutination phenomenon. 27 strains could not be detected. 26 Mongolian drugs, such as rhubarb, Forsythia suspensa and Fructus chebula, were tested by in vitro bacteriostasis test and orthogonal method. The Mongolian medicine compound was selected and optimized in dosage. The four groups were compound 鈪，
本文编号：2147879
本文链接：https://www.wllwen.com/yixuelunwen/dongwuyixue/2147879.html
