广西首例猪圆环病毒3型的发现及其衣壳蛋白序列分析

发布时间：2018-07-27 12:51

【摘要】：【目的】明确猪圆环病毒3型(Porcine circovirustype 3,PCV3)在广西猪群中的致病性及流行病学特点,为有效防控其暴发流行提供参考依据。【方法】采用PCR对广西某猪场腹泻死亡仔猪的病料样品进行PCV3检测,并对其唯一结构蛋白质(Cap)的结构进行同源模拟,构建遗传进化树;同时在线(http://www.cbs.dtu.dk/services/)预测Cap蛋白信号肽、糖基化位点和B细胞抗原表位。【结果】在广西某猪场腹泻死亡仔猪的病料样品中检测到PCV3。PCR扩增产物经核苷酸序列测定分析后截取Cap基因序列(645 bp),命名为PCV3/CN/Guangxi001/2017。PCV3/CN/Guangxi001/2017与13株国内PCV3毒株和5株美国PCV3毒株的Cap基因序列同源性分别在96.9%~99.4%和98.3%~99.5%,属于3b亚群;而与PCV1毒株和PCV2毒株的核苷酸序列同源性只有43.0%和45.8%,且B细胞抗原表位差异明显,在PCV1特有的B细胞抗原表位(92~103 aa)、PCV2特有的B细胞抗原表位(69~83 aa、117~131 aa和231~233 aa)及PCV1、PCV2共有的B细胞抗原表位(156~162 aa和179~192 aa)均无相似性。【结论】广西猪群中已存在PCV3感染,鉴于PCV2与PCV3间无交叉免疫保护特性,实际生产中应通过加强清洁消毒、灭鼠杀虫、定期监测、自繁自养等生物安全措施防控PCV3暴发流行。
[Abstract]:[objective] to study the pathogenicity and epidemiological characteristics of porcine circovirus type 3 (Porcine circovirustype 3) in Guangxi pig population. [methods] PCR was used to detect the disease material of dead piglets with diarrhea in a pig farm in Guangxi, and the structure of its unique structural protein (Cap) was homologous simulated to construct genetic evolution tree. Simultaneous online (http://www.cbs.dtu.dk/services/) prediction of Cap protein signal peptides, Glycosylation sites and B cell antigen epitopes. [results] PCV3.PCR amplification products were detected in the samples of piglets with dead piglets with diarrhea in a pig farm in Guangxi. After nucleotide sequence analysis, the Cap gene sequences (645 bp), named PCV3/CN/Guangxi001/2017.PCV3/CN/Guangxi001/2017 and 13%) were obtained. The homology of Cap gene sequence of domestic PCV3 strain and 5 American PCV3 strain was 99.4% and 98.3%, respectively, belonging to 3b subgroup. The nucleotide sequence homology was only 43.0% and 45.8% with PCV1 and PCV2 strains, and the B cell epitopes were significantly different. There was no similarity in B cell epitope (92103aa), B cell antigen epitope (6983aaaA1a) and B cell antigen epitope (179192aa) shared by PCV1 and PCV1. [conclusion] PCV3 infection has been found in Guangxi pig population, and the B cell antigenic epitope of PCV1 / PCV2 (156o162aa and 179192aa) is not similar to that of PCV2-specific B cell epitope. [conclusion] there is no similarity between the B cell antigen epitopes of PCV2 and that of PCV2. [conclusion] In view of the fact that there is no cross immune protection between PCV2 and PCV3, it is necessary to prevent and control the outbreak of PCV3 by strengthening cleaning and disinfection, killing rats and insects, regularly monitoring and self-propagating and self-nourishing.
【作者单位】：广西兽医研究所/广西兽医生物技术重点实验室;
【基金】：广西科学研究与技术开发计划项目(桂科重14121003-3-1) 广西水产畜牧兽医局科技项目(桂渔牧科201633002)
【分类号】：S852.651

【相似文献】