猪源大肠杆菌脂多糖的提
发布时间：2018-07-27 13:09
【摘要】：为了从猪源大肠杆菌中获得纯度高、产率高的脂多糖(lipopolysaccharides,LPS),并评价其毒力,本试验采集仔猪腹泻粪便作为病料,进行细菌分离鉴定和16SrDNA鉴定,采用热酚水法提取细菌的LPS,通过DNaseⅠ、RNaseA、蛋白酶K和醇沉法对LPS进行纯化,通过苯酚硫酸法、Bradford法和紫外分光光度法测定LPS多糖、蛋白及核酸含量,鲎试剂定量法测定其活性,采用小鼠急性毒性试验方法测定小鼠半数致死量(LD50)。结果显示,分离获得一株具有高致病性的猪源大肠杆菌,纯化的猪源大肠杆菌LPS平均产率为3.74%,其中多糖含量为13.40%,蛋白含量为0.48%,核酸含量为0.06%,主要条带集中在14~160ku范围内,鲎试剂定量法测定其活性为1.21×107 EU/mg,提取LPS对小鼠的LD50为31.86mg/kg。该猪场仔猪腹泻是由致病性大肠杆菌引起,获得其毒力因子LPS纯度高、产率高、毒力强,这将为临床防制猪大肠杆菌病及LPS提取纯化提供理论依据。
[Abstract]:In order to obtain lipopolysaccharide (LPS) from porcine Escherichia coli with high purity and yield and to evaluate its virulence, the feces of piglet diarrhea were collected as diseased materials, and the bacteria were isolated and identified by 16SrDNA. The LPS was purified by DNase 鈪，
本文编号：2147939
[Abstract]:In order to obtain lipopolysaccharide (LPS) from porcine Escherichia coli with high purity and yield and to evaluate its virulence, the feces of piglet diarrhea were collected as diseased materials, and the bacteria were isolated and identified by 16SrDNA. The LPS was purified by DNase 鈪，
本文编号：2147939
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