1日龄牦牛小肠黏膜免疫相关细胞的数量变化研究
发布时间：2018-07-29 19:14
【摘要】：牦牛(Bos Grunniens)作为高原地区特有物种,以高寒草地牧草为食,其高寒、低氧的恶劣生活环境使得腹泻疾病发生呈常态化,尤其是1日龄牦牛腹泻疾病尤为严重。肠黏膜免疫系统是机体重要的免疫屏障,肠黏膜免疫相关细胞在肠黏膜发挥免疫效应中起着重要的作用。本研究旨在对1日龄牦牛小肠黏膜免疫相关细胞的数量变化进行研究。采用免疫组织化学和组织学特殊染色技术,分别对小肠的上皮内淋巴细胞、固有层淋巴细胞、上皮内杯状细胞和肥大细胞分布特点和数量变化进行研究,对各肠段分泌的粘液性质进行判定。研究结果显示:上皮内淋巴细胞数量由十二指肠向空肠、回肠呈依次减少的趋势,差异极显著(P0.01);固有层淋巴细胞数量、肥大细胞数量从十二指肠向回肠呈依次递增的趋势,差异极显著(P0.01);上皮内杯状细胞从十二指肠到回肠呈增长趋势,十二指肠与空肠差异显著(P0.05),但二者与回肠差异极显著(p0.01);十二指肠上皮杯状细胞分泌中性粘液,而空肠、回肠分泌酸性粘液或中性和酸性混合性粘液;十二指肠腺靠近黏膜层分泌中性粘液,靠近肌层分泌酸性粘液;小肠腺均分泌有中性和酸性混合性粘液。1日龄牦牛小肠不同肠段黏膜相关免疫细胞数量的不同变化,显示了小肠黏膜为机体天然屏障的重要组成部分。
[Abstract]:Yak (Bos Grunniens), as a endemic species in plateau area, feeds on herbage in alpine grassland. Its harsh living environment of high cold and low oxygen makes the diarrhea disease normal, especially the diarrhea disease of yak at the age of 1 day. Intestinal mucosal immune system is an important immune barrier, and intestinal mucosal immune-associated cells play an important role in the immune effect of intestinal mucosa. The purpose of this study was to study the changes of the number of mucosal immune-associated cells in the small intestine of 1-day-old yak. The distribution and quantity of intraepithelial lymphocytes, lamina propria lymphocytes, intraepithelial goblet cells and mast cells in the small intestine were studied by immunohistochemical and histological special staining techniques. The properties of mucus secreted by each intestinal segment were determined. The results showed that the number of intraepithelial lymphocytes decreased from duodenum to jejunum and ileum decreased (P0.01), and the number of lymphocytes in lamina propria and mast cell increased from duodenum to ileum. The intraepithelial goblet cells increased from duodenum to ileum, the difference between duodenum and jejunum was significant (P0.05), but the difference between duodenal goblet cells and ileum was very significant (p0.01), duodenal goblet cells secreted neutrophilic mucus, while jejunum, duodenal goblet cells secreted neutrophilic mucus and jejunum. Ileum secreted acidic mucus or mixed neutral and acidic mucus, duodenal gland secreted neutral mucus near mucosal layer and acidic mucus near muscle layer. The intestinal glands secreted neutral and acidic mixed mucus. 1. The number of mucosal associated immune cells in different intestinal segments of yak small intestine showed that intestinal mucosa was an important part of the natural barrier of the body.
【学位授予单位】：甘肃农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S823.85
本文编号：2153717
[Abstract]:Yak (Bos Grunniens), as a endemic species in plateau area, feeds on herbage in alpine grassland. Its harsh living environment of high cold and low oxygen makes the diarrhea disease normal, especially the diarrhea disease of yak at the age of 1 day. Intestinal mucosal immune system is an important immune barrier, and intestinal mucosal immune-associated cells play an important role in the immune effect of intestinal mucosa. The purpose of this study was to study the changes of the number of mucosal immune-associated cells in the small intestine of 1-day-old yak. The distribution and quantity of intraepithelial lymphocytes, lamina propria lymphocytes, intraepithelial goblet cells and mast cells in the small intestine were studied by immunohistochemical and histological special staining techniques. The properties of mucus secreted by each intestinal segment were determined. The results showed that the number of intraepithelial lymphocytes decreased from duodenum to jejunum and ileum decreased (P0.01), and the number of lymphocytes in lamina propria and mast cell increased from duodenum to ileum. The intraepithelial goblet cells increased from duodenum to ileum, the difference between duodenum and jejunum was significant (P0.05), but the difference between duodenal goblet cells and ileum was very significant (p0.01), duodenal goblet cells secreted neutrophilic mucus, while jejunum, duodenal goblet cells secreted neutrophilic mucus and jejunum. Ileum secreted acidic mucus or mixed neutral and acidic mucus, duodenal gland secreted neutral mucus near mucosal layer and acidic mucus near muscle layer. The intestinal glands secreted neutral and acidic mixed mucus. 1. The number of mucosal associated immune cells in different intestinal segments of yak small intestine showed that intestinal mucosa was an important part of the natural barrier of the body.
【学位授予单位】：甘肃农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S823.85
【参考文献】
相关期刊论文 前2条
1 史玉兰,段相林;杯状细胞的研究进展[J];解剖科学进展;2001年04期
2 林纪胜,徐建国;粘膜免疫的研究进展[J];中国人兽共患病杂志;1999年04期
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