百里香酚对山羊子宫内膜上皮细胞的体外毒活性研究
发布时间：2018-08-03 19:35
【摘要】：为了探究百里香酚对山羊子宫内膜上皮细胞(goat endometrial epithelial cells,g EECs)的毒性作用,采用不同浓度的百里香酚作用于g EECs后,观测其对细胞生长曲线,细胞形态学变化,以及细胞膜结构完整性的影响。结果表明,百里香酚对EECs的毒性作用与其浓度呈剂量依赖效应(y=-0.008x+1.2202,R~2=0.9767),即随着药物浓度的增加细胞的增殖抑制率增大,药物对细胞的半数增殖抑制率(IC50)为100μg/m L。当50、100μg/m L的百里香酚作用于EECs时,g EECs的细胞核、细胞浆的形态均未产生明显变化,但当150μg/m L的百里香酚作用于g EECs时,细胞膜破裂,细胞死亡;而且药物浓度≥100μg/m L时细胞LDH的释放量显著高于正常细胞组,g EECs细胞膜的完整性受到破坏。百里香酚作用于山羊子宫内膜上皮细胞后呈现一定的毒性作用,且毒性作用和药物的浓度有关。百里香酚作用于g EECs的安全范围在0~100μg/m L,研究结果为百里香酚治疗奶牛等经济动物的炎症性疾病的临床用药浓度提供理论依据。
[Abstract]:In order to investigate the toxicity of thyme to (goat endometrial epithelial cells g EECs in goat endometrial epithelial cells, the growth curve and morphological changes of the cells were observed after treated with different concentrations of thymol on g EECs. And the effect of cell membrane structural integrity. The results showed that the toxic effect of thyme phenol on EECs was dose-dependent (YP0. 008x 1. 2202RX 0.9767), that is, with the increase of drug concentration, the half proliferation inhibition rate (IC50) of thymol on EECs was 100 渭 g / mL. When 50100 渭 g / mL thymol acted on the nucleus of EECs, the morphology of cytoplasm of G EECs did not change significantly, but when 50100 渭 g / mL thymol acted on g EECs, the cell membrane broke down and the cells died. Moreover, the release of LDH in the cells with drug concentration 鈮，
本文编号：2162790
[Abstract]:In order to investigate the toxicity of thyme to (goat endometrial epithelial cells g EECs in goat endometrial epithelial cells, the growth curve and morphological changes of the cells were observed after treated with different concentrations of thymol on g EECs. And the effect of cell membrane structural integrity. The results showed that the toxic effect of thyme phenol on EECs was dose-dependent (YP0. 008x 1. 2202RX 0.9767), that is, with the increase of drug concentration, the half proliferation inhibition rate (IC50) of thymol on EECs was 100 渭 g / mL. When 50100 渭 g / mL thymol acted on the nucleus of EECs, the morphology of cytoplasm of G EECs did not change significantly, but when 50100 渭 g / mL thymol acted on g EECs, the cell membrane broke down and the cells died. Moreover, the release of LDH in the cells with drug concentration 鈮，
本文编号：2162790
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