饲粮营养限制对羔羊肠道组织形态以及血清胰岛素样生长因子-1和胰高血糖素样-2浓度的影响

发布时间：2018-08-04 17:35

【摘要】：本试验旨在研究饲粮营养限制对羔羊肠道组织形态及血清中胰岛素样生长因子-1(IGF-1)和胰高血糖素样-2(GLP-2)浓度的影响。选取64只17日龄湖羊羔羊随机分为4组,饲喂不同营养水平的代乳品和开食料,分别为对照(CON)组、20%蛋白质限制(PR)组、20%能量限制(ER)组、20%蛋白质+20%能量限制(BR)组,每组4个重复,每重复4只羔羊,公母各占1/2。于羔羊20日龄、40日龄和60日龄采集血清用于测定IGF-1和GLP-2的浓度,40日龄和60日龄时每组分别选取4只羔羊进行屠宰,屠宰后取十二指肠、空肠和回肠组织观察小肠组织形态结构。结果表明:1)PR组、ER组、BR组40日龄小肠重量显著低于CON组(P0.05),到60日龄仅ER组羔羊小肠重显著低于CON组(P0.05),小肠重量降低主要由于空肠重量的降低。2)PR组、ER组、BR组40日龄十二指肠、空肠绒毛高度及60日龄十二指肠绒毛高度显著低于CON组(P0.05),ER组、BR组60日龄空肠绒毛高度显著低于CON组(P0.05)。3)BR组40日龄十二指肠绒毛高度/隐窝深度显著低于CON组(P0.05)。4)ER组60日龄血清GLP-2浓度显著低于其他3组(P0.05),4组间血清IGF-1浓度无显著差异(P0.05)。综上所述,饲粮营养限制抑制了羔羊十二指肠、空肠组织形态发育,同时能量限制可降低血清GLP-2的浓度。
[Abstract]:The aim of this study was to investigate the effects of dietary nutrition restriction on intestinal morphology and serum concentrations of insulin-like growth factor-1 (IGF-1) and glucagon like -2 (GLP-2) in lambs. Sixty-four 17-day-old Hu sheep were randomly divided into 4 groups, fed with milk substitute and diet with different nutritional levels. They were divided into two groups: control (CON) group (20% protein), (PR) group (20% energy limit), (ER) group (20% protein), 20% protein (20%) energy limited (BR) group, 4 replicates in each group. For every 4 lambs repeated, males and females accounted for 1 / 2 / 2 of each. Serum samples were collected at 20 and 60 days of age to determine the concentrations of IGF-1 and GLP-2. Four lambs from each group were slaughtered at 40 and 60 days of age respectively. The duodenum, jejunum and ileum were collected after slaughter for observation of intestinal morphology. The results showed that the weight of small intestine of ER group was significantly lower than that of CON group (P0.05), and that of ER group was significantly lower than that of CON group (P0.05) at 60 days old. The decrease of intestinal weight was mainly due to the decrease of jejunum weight. The height of jejunum villi and duodenal villi at 60 days of age were significantly lower than those in CON group (P0.05). The villi height of 60 days old in ER group was significantly lower than that in CON group (P0.05). The villi height / crypt depth in 40 day old group in Br group was significantly lower than that in CON group (P0.05) and the depth of crypt in ER group was significantly lower than that in ER group (P0.05). There was no significant difference in serum GLP-2 concentration between 4 groups (P0.05). To sum up, dietary nutrition restriction inhibited the development of duodenum and jejunum of lambs, and energy restriction decreased the concentration of serum GLP-2.
【作者单位】：中国农业科学院饲料研究所农业部饲料生物技术重点开放实验室;南京农业大学;
【基金】：公益性科研专项"南方地区幼龄草食畜禽饲养技术研究(201303143) 国家肉羊产业技术体系建设专项资金(CARS-39)
【分类号】：S826.5

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