Ghrelin对绵羊早期体外胚胎发育调节机制的初步研究

发布时间：2018-08-06 08:48

【摘要】：【目的】探讨Ghrelin对绵羊早期胚胎发育的调节机制。【方法】将绵羊体外受精早期胚胎在Ghrelin组(300,1 000,10 000ng/mL)、Ghrelin受体拮抗剂D-Lys3-GHRP-6组(10,200,400ng/mL)、D-Lys3-GHRP-6+Ghrelin组(D-Lys3-GHRP-6 400ng/mL,Ghrelin 300,1 000和2 000ng/mL)、空白对照(不加D-Lys3-GHRP-6和Ghrelin)4个组别的胚胎发育液中进行发育培养,采用荧光定量PCR技术分别对各处理不同发育阶段(2-细胞期、4-细胞期、8-细胞期和16-细胞期)绵羊早期胚胎中ERK1、ERK2、P90rsk、Bcl-2、Bax mRNA的表达情况进行检测。【结果】与空白对照组相比,在添加Ghrelin组ERK1、ERK2、P90rsk、Bcl-2基因mRNA的表达表现为显著性上调(P0.05),且这种上调作用可被D-Lys3-GHRP-6所拮抗;在添加Ghrelin组Bax基因mRNA的表达无显著性变化,但在D-Lys3-GHRP-6组Bax基因mRNA的表达表现为显著性上调(P0.05)。【结论】Ghrelin可级联激活MAPK信号通路,抑制促凋亡基因Bax的表达、促进抗凋亡基因Bcl-2的表达,进而促进绵羊早期胚胎的发育。
[Abstract]:[objective] to investigate the regulatory mechanism of Ghrelin on the development of sheep early embryos. [methods] the sheep early embryos were fertilized in vitro in Ghrelin group (300,000min / 10 000ng/mL) with ghrelin receptor antagonist D-Lys3-GHRP-6 group (10200400ng/mL), D-Lys3-GHRP-6 Ghrelin group (D-Lys3-GHRP-6 400ngr / mL) ghrelin 3001 000 and 2 000ng/mL, blank control (without D-Lys3-GHRP-6 and Ghrelin) 4. In the embryonic development fluid of each group, Fluorescence quantitative PCR technique was used to detect the expression of ERK1 and ERK2P90rskP90rskPcl-2Bcl-2Bcl-2 Bax mRNA in sheep embryos at different developmental stages (2-cell stage, 2-cell stage, 8-cell stage and 16-cell stage). [results] compared with the control group, the expression of ERK1 and ERK2P90rsk2 Bcl-2Bcl-2Bcl-2Bax mRNA was detected by fluorescence quantitative PCR. The expression of mRNA of ERK1 + ERK2 + P90rsktrol Bcl-2 gene was significantly up-regulated in Ghrelin group (P0.05), and this up-regulation could be antagonized by D-Lys3-GHRP-6, but the expression of Bax gene mRNA in Ghrelin group had no significant change. But in D-Lys3-GHRP-6 group, the expression of Bax gene mRNA was significantly up-regulated (P0.05). [conclusion] Ghrelin can cascade activate MAPK signaling pathway, inhibit the expression of pro-apoptotic gene Bax, promote the expression of anti-apoptotic gene Bcl-2, and then promote the development of sheep early embryo.
【作者单位】：内蒙古农业大学兽医学院;内蒙古自治区农牧业科学院畜牧研究所;新疆农垦科学院畜牧兽医研究所;
【基金】：国家自然科学基金项目(30860201)
【分类号】：S826

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