五加芪水煎液对小鼠和雏鸡免疫功能的影响
发布时间：2018-08-06 08:02
【摘要】：本试验以研究五加芪水煎液分别对小鼠和雏鸡免疫功能的作用为目的。试验由体外试验和体内试验两部分组成。体外试验:小鼠脾脏淋巴细胞与不同浓度的五加芪水煎液混合培养48 h后,采用MTT法检测淋巴细胞增殖情况。T淋巴细胞分泌的IL-2和IFN-γ的浓度是通过ELISA试剂盒来进行检测的。体内试验:将7周龄的昆明系雌性小鼠进行随机分组,10只/组。试验组分为正常小鼠组(包括:正常对照组、高、中、低剂量组)和免疫抑制小鼠组(包括:正常对照组、Hy+蒸馏水组、Hy+高、中、低剂量组),连续灌胃7 d后,测定各组小鼠的免疫器官指数、碳粒廓清指数、血清溶血素水平以及脾内抗体形成细胞(AFC)的变化。将雏鸡随机分为4组,A-D组,50只/组。A-D组在6日龄和20日龄分别免疫新城疫疫苗,B-D组在首次免疫前两天分别灌服高、中、低剂量的中药,每只0.5 m L,A组每只灌服0.5 m L蒸馏水,连续7 d,测定雏鸡免疫器官指数、新城疫抗体水平、巨噬细胞吞噬活性、肠道Ig A分泌细胞的变化。试验结果表明,五加芪水煎液单独刺激能显著促进T、B淋巴细胞增殖,并且4倍稀释组的五加芪水煎液能显著促进T淋巴细胞分泌IL-2,原液组五加芪水煎液能显著促进T淋巴细胞分泌IFN-γ(P0.01)。五加芪水煎液协同PHA能显著促进T淋巴细胞增殖,五加芪水煎液4倍稀释后能够促进T淋巴细胞分泌的IL-2,使其含量显著高于细胞对照组与PHA对照组,五加芪水煎液2倍稀释后能够显著提高T淋巴细胞分泌的IFN-γ含量,与细胞对照组与PHA对照组相比有显著差异(P0.01)。五加芪水煎液协同LPS可以促进B淋巴细胞增殖,与对照组有显著差异(P0.01)。高、中剂量五加芪水煎液使正常小鼠脾指数显著地升高(P0.01),3个剂量的五加芪水煎液均能使正常小鼠胸腺指数和碳粒廓清指数增加明显(P0.01或P0.05),正常高剂量组小鼠血清溶血素水平明显高于正常对照组而且存在显著差异(P0.01),3个剂量组脾内抗体形成细胞数量与正常对照组相比差异不明显(P0.05)。免疫抑制小鼠组中各中药水煎液组小鼠的免疫器官指数、碳粒廓清指数、血清溶血素水平和脾内抗体形成细胞数量均明显高于Hy+蒸馏水组(P0.01或P0.05),达到正常对照组水平(P0.05),说明五加芪水煎液使免疫抑制小鼠的免疫功能恢复至正常水平。15日龄时,高、中剂量组脾指数显著高于对照组(P0.05),各五加芪水煎液组胸腺指数和法氏囊指数均明显地升高,与对照组有显著差异(P0.01或P0.05)。23日龄,各剂量五加芪水煎液均能显著增加雏鸡脾指数和胸腺指数,并且显著差异于对照组(P0.01或P0.05),高、中剂量组雏鸡法氏囊指数显著高于对照组(P0.05)。31日龄,各剂量的五加芪水煎液能明显升高雏鸡的脾指数(P0.01或P0.05);而各剂量五加芪水煎液组胸腺指数和法氏囊指数与对照组无显著差异(P0.05)。15日龄,3个浓度的五加芪水煎液均能使雏鸡抗体水平显著升高(P0.01或P0.05);23和31日龄,只有高剂量组雏鸡的ND抗体水平显著高于对照组(P0.05)。16日龄时,3个剂量组吞噬指数显著高于对照组(P0.01);在24日龄时,各浓度组吞噬指数与对照组差异不显著(P0.05)。在15、23以及31日龄时,各用药组十二指肠和空肠中Ig A分泌细胞数量均多于对照组,有显著差异(P0.01或P0.05)。五加芪水煎液能够提高小鼠和雏鸡的免疫功能。
[Abstract]:The purpose of this experiment was to study the effect of Wujia Qi Decoction on immune function of mice and chicks. The experiment was made up of two parts in vitro and in vivo. In vitro test, the spleen lymphocytes of mice were mixed with different concentration of Wujia Qi water decoction for 48 h, and the.T lymphocyte secretion was detected by MTT method. The concentration of IL-2 and IFN- gamma was detected by the ELISA kit. In vivo test: 7 weeks old Kunming female mice were randomly divided into 10 groups. The test group was divided into normal mice group (including normal control, high, middle, low dose group) and immunosuppressive mice group (including: normal control, Hy+ distilled water group, Hy+ high, medium, low dose. After 7 d continuous gavage, the immune organ index, carbon clearance index, serum hemolysin level, and intravenic antibody forming cell (AFC) were measured in each group. The chickens were randomly divided into 4 groups, A-D group and 50 group.A-D groups at 6 and 20 days of age, respectively, and the B-D group was given high level in the first two days before the first immunization. The low dose of traditional Chinese medicine, each 0.5 m L, group A each filled with 0.5 m L distilled water and 7 d continuously, measured the immune organ index, the antibody level of Newcastle disease, the macrophage phagocytosis activity, and the intestinal Ig A secretory cells. The experimental results showed that the individual stimulation of Wujia Qi Decoction could significantly promote T, B lymphocyte proliferation, and five of the 4 times dilution group. The decoction of Astragalus can significantly promote the secretion of IL-2 in T lymphocytes. The decoction of Wujia Qi in the original solution can promote the secretion of IFN- gamma (P0.01) in T lymphocytes. The synergistic effect of Wujia Qi Decoction with PHA can significantly promote the proliferation of T lymphocytes. After 4 times dilution of Wujia Qi water decoction, the secretion of IL-2 in T lymphocytes can be promoted, and the content of the decoction can be significantly higher than that of the cell control. Group and PHA control group, after 2 times dilution of Wujia Qi water decoction can significantly increase the content of IFN- gamma secreted by T lymphocyte, and there is significant difference between the control group and the PHA control group (P0.01). The synergistic effect of Wujia Qi Decoction with LPS can promote the proliferation of B lymphocyte, and there is a significant difference between the control group and the control group (P0.01). The medium dose of Wujia Qi water decoction is normal. The spleen index of mice increased significantly (P0.01). The 3 doses of Wujia Qi Decoction could increase the thymus index and carbon particle clearance index in normal mice (P0.01 or P0.05). The serum hemolysin level of the normal high dose group was significantly higher than that of the normal control group (P0.01), and the number of antibody forming cells in the spleen in the 3 dosage groups was significantly different. The immune organ index, carbon clearance index, serum hemolysin level and the number of antibody forming cells in the spleen were significantly higher than that of the Hy+ distilled water group (P0.01 or P0.05) in the mice of the immunosuppressed group, which was significantly higher than that in the normal control group (P0.05), and reached the normal control level (P0.05), indicating the decoction of Wujia Qi. When the immune function of the immunosuppressed mice was restored to the normal level of.15 days of age, the spleen index of the medium dose group was significantly higher than that of the control group (P0.05). The thymus index and the bursa index of the Wujia Decoction group were significantly increased, and there was significant difference from the control group (P0.01 or P0.05).23 days of age, and all doses of Wujia Qi water decoction could significantly increase the embryo. The chicken spleen index and thymus index were significantly different from those in the control group (P0.01 or P0.05), and the fowl bursa index in the middle dose group was significantly higher than that of the control group (P0.05).31 days old. The spleen index (P0.01 or P0.05) of the chicks could be significantly increased in each dose of Wujia Qi water decoction (P0.01 or P0.05). No significant difference (P0.05).15 days of age, 3 concentration of Wujia Qi Decoction can significantly increase the level of chicken antibody (P0.01 or P0.05); 23 and 31 days of age, only high dose group of chicken ND antibody level is significantly higher than the control group (P0.05).16 days of age, 3 dose group phagocytic index is significantly higher than the control group (P0.01); at 24 days of age, the concentration of each concentration There was no significant difference between the group phagocytosis index and the control group (P0.05). At 15,23 and 31 day old, the number of Ig A secreting cells in the duodenum and jejunum of each group were more than those in the control group (P0.01 or P0.05). The decoction of Wujia Qi water could improve the immune function of mice and chicks.
【学位授予单位】：河北农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S853.7
本文编号：2167047
[Abstract]:The purpose of this experiment was to study the effect of Wujia Qi Decoction on immune function of mice and chicks. The experiment was made up of two parts in vitro and in vivo. In vitro test, the spleen lymphocytes of mice were mixed with different concentration of Wujia Qi water decoction for 48 h, and the.T lymphocyte secretion was detected by MTT method. The concentration of IL-2 and IFN- gamma was detected by the ELISA kit. In vivo test: 7 weeks old Kunming female mice were randomly divided into 10 groups. The test group was divided into normal mice group (including normal control, high, middle, low dose group) and immunosuppressive mice group (including: normal control, Hy+ distilled water group, Hy+ high, medium, low dose. After 7 d continuous gavage, the immune organ index, carbon clearance index, serum hemolysin level, and intravenic antibody forming cell (AFC) were measured in each group. The chickens were randomly divided into 4 groups, A-D group and 50 group.A-D groups at 6 and 20 days of age, respectively, and the B-D group was given high level in the first two days before the first immunization. The low dose of traditional Chinese medicine, each 0.5 m L, group A each filled with 0.5 m L distilled water and 7 d continuously, measured the immune organ index, the antibody level of Newcastle disease, the macrophage phagocytosis activity, and the intestinal Ig A secretory cells. The experimental results showed that the individual stimulation of Wujia Qi Decoction could significantly promote T, B lymphocyte proliferation, and five of the 4 times dilution group. The decoction of Astragalus can significantly promote the secretion of IL-2 in T lymphocytes. The decoction of Wujia Qi in the original solution can promote the secretion of IFN- gamma (P0.01) in T lymphocytes. The synergistic effect of Wujia Qi Decoction with PHA can significantly promote the proliferation of T lymphocytes. After 4 times dilution of Wujia Qi water decoction, the secretion of IL-2 in T lymphocytes can be promoted, and the content of the decoction can be significantly higher than that of the cell control. Group and PHA control group, after 2 times dilution of Wujia Qi water decoction can significantly increase the content of IFN- gamma secreted by T lymphocyte, and there is significant difference between the control group and the PHA control group (P0.01). The synergistic effect of Wujia Qi Decoction with LPS can promote the proliferation of B lymphocyte, and there is a significant difference between the control group and the control group (P0.01). The medium dose of Wujia Qi water decoction is normal. The spleen index of mice increased significantly (P0.01). The 3 doses of Wujia Qi Decoction could increase the thymus index and carbon particle clearance index in normal mice (P0.01 or P0.05). The serum hemolysin level of the normal high dose group was significantly higher than that of the normal control group (P0.01), and the number of antibody forming cells in the spleen in the 3 dosage groups was significantly different. The immune organ index, carbon clearance index, serum hemolysin level and the number of antibody forming cells in the spleen were significantly higher than that of the Hy+ distilled water group (P0.01 or P0.05) in the mice of the immunosuppressed group, which was significantly higher than that in the normal control group (P0.05), and reached the normal control level (P0.05), indicating the decoction of Wujia Qi. When the immune function of the immunosuppressed mice was restored to the normal level of.15 days of age, the spleen index of the medium dose group was significantly higher than that of the control group (P0.05). The thymus index and the bursa index of the Wujia Decoction group were significantly increased, and there was significant difference from the control group (P0.01 or P0.05).23 days of age, and all doses of Wujia Qi water decoction could significantly increase the embryo. The chicken spleen index and thymus index were significantly different from those in the control group (P0.01 or P0.05), and the fowl bursa index in the middle dose group was significantly higher than that of the control group (P0.05).31 days old. The spleen index (P0.01 or P0.05) of the chicks could be significantly increased in each dose of Wujia Qi water decoction (P0.01 or P0.05). No significant difference (P0.05).15 days of age, 3 concentration of Wujia Qi Decoction can significantly increase the level of chicken antibody (P0.01 or P0.05); 23 and 31 days of age, only high dose group of chicken ND antibody level is significantly higher than the control group (P0.05).16 days of age, 3 dose group phagocytic index is significantly higher than the control group (P0.01); at 24 days of age, the concentration of each concentration There was no significant difference between the group phagocytosis index and the control group (P0.05). At 15,23 and 31 day old, the number of Ig A secreting cells in the duodenum and jejunum of each group were more than those in the control group (P0.01 or P0.05). The decoction of Wujia Qi water could improve the immune function of mice and chicks.
【学位授予单位】：河北农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S853.7
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