中药组方“回乳康”对回乳期奶牛血清E、P4和TGF-β1含量的影响

发布时间：2018-08-06 21:57

【摘要】：【目的】探究中药组方"回乳康"对回乳期奶牛血清雌激素(Estrogen,E)、孕酮(Progesterone,P4)和转化生长因子β1(Transforming growth factorβ1,TGF-β1)含量的影响。【方法】选取即将干乳日产奶量为(15.42±0.71)kg的健康奶牛80头,随机分为4组,每天饲喂0(对照组)、400、500和600 g"回乳康"中药,采用逐渐干奶法回乳,分别在回乳第0、1、3、5、7、9和11天采集奶牛尾静脉血,并记录奶牛单日产奶量,采用ELISA法检测血清E、P4和TGF-β1含量。【结果】0、400、500和600 g处理回乳时间分别为11、7、5和5 d;各组奶牛血清E含量在回乳期均随时间推移呈下降趋势,对照组下降趋势较中药组慢;对照组奶牛血清P4含量在回乳期变化不显著,而中药组奶牛血清P4含量均随时间推移呈上升趋势,在回乳期之后下降,但下降后仍极显著高于对照组;各处理回乳期奶牛血清TGF-β1含量均呈上升趋势,而在回乳期之后中药组奶牛血清TGF-β1含量呈下降趋势;回乳期奶牛血清E、TGF-β1含量和日产奶量两两间均呈极显著相关,血清P4含量与血清E、TGF-β1含量、日产奶量均无显著相关性。【结论】在奶牛回乳过程中,添加中药组方"回乳康"能显著促进回乳期奶牛回乳,E是奶牛回乳的负调控激素,TGF-β1是正调控因子,而P4对回乳无明显影响。
[Abstract]:[objective] to investigate the effects of "Huirukang", a traditional Chinese medicine prescription, on serum estrogen E, progesterone P4 and transforming growth factor 尾 1 (Transforming growth factor 尾 1TGF- 尾 1. [methods] 80 healthy cows with milk yield of (15.42 卤0.71) kg on dry milk day were randomly divided into 4 groups. Four hundred and six hundred g of "Huirukang" were fed daily as control group. The milk was rebrewed with the method of gradual dry milk. The venous blood was collected from the tail vein of the cow on the 1st and 11th days, respectively, and the milk yield was recorded on a single day. The serum levels of EfP 4 and TGF- 尾 1 were detected by ELISA method. [results] the time of milk resuscitation in 0400500 g and 600g groups was 110.75 and 5 days, respectively, and the serum E content in each group showed a decreasing trend with the passage of time, while that in the control group was slower than that in the traditional Chinese medicine group. There was no significant change in serum P4 content in the control group, while the serum P4 content in the Chinese medicine group increased with the passage of time, and decreased after the resuckling period, but it was still significantly higher than that in the control group. The content of TGF- 尾 1 in serum of dairy cattle in each remilk stage showed an increasing trend, but the TGF- 尾 1 content in serum of Chinese medicine group decreased after relactation, and the content of serum TGF- 尾 1 and daily milk yield were significantly correlated with each other during the period of milk resuscitation, and the content of TGF- 尾 1 in serum of traditional Chinese medicine group was significantly correlated with the daily milk yield. There was no significant correlation between serum P4 content and serum EG TGF- 尾 1 content and daily milk yield. [conclusion] during the process of milk resuscitation, there was no significant correlation between serum P4 content and serum EGF-尾 1 content. The addition of "Huiru Kang", a traditional Chinese medicine prescription, could significantly promote that the negative regulating hormone TGF- 尾 _ 1 was a positive regulatory factor, while P4 had no obvious effect on the remilk of dairy cows.
【作者单位】：四川农业大学动物医学院奶牛疾病研究中心/动物疫病与人类健康四川省重点实验室;江苏农牧科技职业学院;
【基金】：四川省教育厅重点项目(15ZA0024) 江苏农牧科技职业学院产业发展关键技术创新项目(NSF201604) 四川农业大学双支计划项目(03571303);四川农业大学本科科研兴趣培养计划项目(KY2016062)
【分类号】：S853.7

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