新疆野生荒漠肉苁蓉粗多糖对口蹄疫疫苗免疫小鼠的佐剂效应

发布时间：2018-08-07 13:28

【摘要】：探讨新疆野生荒漠肉苁蓉粗多糖(wild Cistanche deserticola Y.C.Ma crude polysaccharides,WCDCP)对口蹄疫(foot-and-mouth disease,FMD)疫苗免疫小鼠的佐剂效应及安全性。用不同剂量的WCDCP分别配伍FMDV灭活抗原及FMD疫苗,皮下注射ICR小鼠,免疫两次。ELISA法检测血清中IgG、IgG1及IgG2a抗体水平;流式细胞术检测淋巴结中CD4+CD44+和CD8+CD44+T细胞表达情况。结果表明,中、高2个剂量的WCDCP均能显著增强FMDV灭活抗原特异性IgG的抗体水平(P0.01);中剂量的WCDCP显著增强了FMD疫苗特异性IgG及其IgG1、IgG2a抗体水平(P0.05),而且具有长效性;WCDCP中剂量可以显著提高淋巴结中CD4+CD44+和CD8+CD44+T细胞的表达水平(P0.05);WCDCP对小鼠的生长没有影响。总之,WCDCP可以长期增强FMD疫苗特异性抗体水平,显著增强T细胞免疫反应,对小鼠的生长无副作用。因此,WCDCP可以作为FMD疫苗的一种安全候选佐剂。
[Abstract]:To study the adjuvant effect and safety of wild Cistanche deserticola polysaccharide (wild Cistanche deserticola Y.C.Ma crude polysaccharide WCDCP on foot-and-mouth disease (FMD) vaccine in mice. Different doses of WCDCP and FMDV inactivated antigen and FMD vaccine were used for subcutaneous injection of ICR mice. The levels of IgG G 1 and IgG2a antibody in serum were detected by Elisa and the expression of CD4 CD44 and CD8 CD44 T cells in lymph nodes were detected by flow cytometry. The results show that, The antibody level of FMDV inactivated antigen-specific IgG was significantly enhanced by high dose of WCDCP (P0.01), and the level of FMD vaccine specific IgG and IgG1G2a antibody was significantly enhanced by middle dose of WCDCP (P0.05), and the medium dose of FMD vaccine specific IgG and IgG1G2a could be significantly increased by the medium dose of FMDV inactivated IgG (P0.01). The expression of CD4 CD44 and CD8 CD44 T cells in lymph nodes (P0.05) had no effect on the growth of mice. In a word, WCDCP can enhance the specific antibody level of FMD vaccine for a long time, and significantly enhance the T cell immune response, and have no side effect on the growth of mice. Therefore, WCDCP can be used as a safety candidate adjuvant for FMD vaccine.
【作者单位】：新疆大学生命科学与技术学院新疆生物资源与基因工程重点实验室;新疆天康畜牧生物技术股份有限公司;
【基金】：新疆维吾尔自治区科技援疆项目(201591150)
【分类号】：S855.3

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