促生长肽结合与代谢功能的活体成像观察及其对猪的促生长作用
发布时间：2018-08-07 13:30
【摘要】：促生长肽是以猪生长激素单克隆抗体为靶标,结合免疫学和计算生物学等相关技术筛选出的一种具有模拟猪生长激素作用的肽类物质。目前已经在细胞水平上证明了该物质具有与猪生长激素相似的生物活性和功能,但促生长肽对动物促生长的有效性验证尚未进行。为观察促生长肽与组织器官的动态结合特性、代谢特性,利用小动物活体成像系统,活体观测大鼠体内荧光标记的促生长功能肽和猪生长激素分布及荧光强度的消长规律,剖解观测具体结合部位情况。研究发现,促生长肽与猪生长激素均能与大鼠体内生长激素的主要靶器官发生特异性结合,两种物质在体内结合的器官和部位相同,但是促生长肽与组织结合的强度较弱;两者在大鼠体内的代谢路径相似,但促生长肽的代谢速度明显快于猪生长激素的现象。为验证促生长肽对动物促生长作用,首先利用试验动物大鼠进行试验方案,注射剂量、注射时期的选择等基础的探索研究。试验数据显示添加促生长肽能够调控体长增长效果明显,表现为动物体长的增长高峰提前;血液中骨钙素含量明显升高(P0.05),碱性磷酸酶有增高趋势,但差异不显著(P0.05)。血液中与骨骼生长密切相关指标的表现提示促生长肽调控体长增长与促进骨骼生长密切相关。添加促生长肽能够调控体重的增长,但与使用剂量密切相关,在试验的各个时间点观测到高中低添加量对体重增长的效果不一。与血液数据对比分析,血清总蛋白呈现增高趋势,IGF-1变化不明显,二者与对照组相比差异不显著(P0.05),而尿素氮显著低于对照组(P0.05)。提示促生长肽调控体重增长与促进蛋白合成相关。开展了促生长肽对断奶仔猪的促生长作用的现场应用研究。试验组分高剂量组和低剂量组。试验结果表明:添加促生长肽能够使猪平均日增重呈现提高趋势,但变化规律不明显。表现为高剂量组在前期和末期效果均达到显著(P0.05),低剂量组后期效果显著(P0.05)。血液指标显示低剂量能够提高仔猪IGF-1的和总蛋白的含量,但差异不显著(P0.05),促进骨钙素增高,减缓碱性磷酸酶浓度的下降,差异显著(P0.05)。研究结果提示:促生长肽对猪的生长具有促进作用,能够促进蛋白的合成及骨生长,但内在的变化机理尚需要深入研究。
[Abstract]:Growth promoting peptide (GFP) is a peptide which is screened by monoclonal antibody against porcine growth hormone and combined with immunology and computational biology. At present, it has been proved that the substance has the same biological activity and function as porcine growth hormone at the cell level, but the effectiveness of GFP on animal growth has not been verified. In order to observe the dynamic binding characteristics and metabolic characteristics of growth promoting peptide with tissues and organs, the distribution and intensity of growth promoting peptide and pig growth hormone labeled by fluorescence in vivo were observed by small animal imaging system in vivo. Dissecting and observing the concrete joint position. It was found that the growth promoting peptide and pig growth hormone could bind specifically to the main target organs of growth hormone in rats. The organs and parts of the two substances bound in vivo were the same, but the intensity of the binding between growth peptide and tissue was weak. The metabolic pathways were similar in rats, but the growth promoting peptide metabolism was faster than that of pig growth hormone. In order to verify the growth promoting effect of GFP on animals, the experimental scheme, injection dose and selection of injection time were used to investigate the effects of GFP on animal growth. The experimental data showed that the growth promoting peptide could regulate the growth of body length obviously, showing that the growth peak of animal body length was earlier; the content of osteocalcin in blood increased significantly (P0.05), and alkaline phosphatase increased, but the difference was not significant (P0.05). The expression of blood closely related to bone growth suggests that growth peptide regulates the growth of body length and promotes bone growth. The effect of high, middle and low supplementation on weight gain was observed at each time point in the experiment, but it was closely related to the use dose. Compared with the blood data, the serum total protein showed an increasing trend and IGF-1 did not change significantly, compared with the control group, there was no significant difference (P0.05), but urea nitrogen was significantly lower than the control group (P0.05). It is suggested that the growth promoting peptide is related to the regulation of body weight gain and the promotion of protein synthesis. The field application study of growth promoting peptide on weaning piglets was carried out. The test group was divided into high dose group and low dose group. The results showed that the average daily gain (ADG) of pigs increased with the addition of GFP, but the rule of change was not obvious. The results showed that the effect of high dose group was significant at the early and end stage (P0.05), and that of low dose group was significant at the later stage (P0.05). Blood index showed that low dose could increase the content of IGF-1 and total protein, but the difference was not significant (P0.05), promote the increase of osteocalcin, slow down the decrease of alkaline phosphatase concentration, the difference was significant (P0.05). The results suggest that GFP can promote the growth of pigs and promote the synthesis of protein and bone growth, but the intrinsic mechanism needs further study.
【学位授予单位】：吉林农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S828.5
本文编号：2170168
[Abstract]:Growth promoting peptide (GFP) is a peptide which is screened by monoclonal antibody against porcine growth hormone and combined with immunology and computational biology. At present, it has been proved that the substance has the same biological activity and function as porcine growth hormone at the cell level, but the effectiveness of GFP on animal growth has not been verified. In order to observe the dynamic binding characteristics and metabolic characteristics of growth promoting peptide with tissues and organs, the distribution and intensity of growth promoting peptide and pig growth hormone labeled by fluorescence in vivo were observed by small animal imaging system in vivo. Dissecting and observing the concrete joint position. It was found that the growth promoting peptide and pig growth hormone could bind specifically to the main target organs of growth hormone in rats. The organs and parts of the two substances bound in vivo were the same, but the intensity of the binding between growth peptide and tissue was weak. The metabolic pathways were similar in rats, but the growth promoting peptide metabolism was faster than that of pig growth hormone. In order to verify the growth promoting effect of GFP on animals, the experimental scheme, injection dose and selection of injection time were used to investigate the effects of GFP on animal growth. The experimental data showed that the growth promoting peptide could regulate the growth of body length obviously, showing that the growth peak of animal body length was earlier; the content of osteocalcin in blood increased significantly (P0.05), and alkaline phosphatase increased, but the difference was not significant (P0.05). The expression of blood closely related to bone growth suggests that growth peptide regulates the growth of body length and promotes bone growth. The effect of high, middle and low supplementation on weight gain was observed at each time point in the experiment, but it was closely related to the use dose. Compared with the blood data, the serum total protein showed an increasing trend and IGF-1 did not change significantly, compared with the control group, there was no significant difference (P0.05), but urea nitrogen was significantly lower than the control group (P0.05). It is suggested that the growth promoting peptide is related to the regulation of body weight gain and the promotion of protein synthesis. The field application study of growth promoting peptide on weaning piglets was carried out. The test group was divided into high dose group and low dose group. The results showed that the average daily gain (ADG) of pigs increased with the addition of GFP, but the rule of change was not obvious. The results showed that the effect of high dose group was significant at the early and end stage (P0.05), and that of low dose group was significant at the later stage (P0.05). Blood index showed that low dose could increase the content of IGF-1 and total protein, but the difference was not significant (P0.05), promote the increase of osteocalcin, slow down the decrease of alkaline phosphatase concentration, the difference was significant (P0.05). The results suggest that GFP can promote the growth of pigs and promote the synthesis of protein and bone growth, but the intrinsic mechanism needs further study.
【学位授予单位】：吉林农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S828.5
【参考文献】
相关期刊论文 前2条
1 伍国耀;;小肠氨基酸的代谢:动物营养的新视角[J];饲料工业;2011年22期
2 熊文中,吴德,杨凤,周安国;生长激素对猪生长性能和胴体品质的影响[J];中国饲料;1998年11期
，本文编号：2170168
本文链接：https://www.wllwen.com/yixuelunwen/dongwuyixue/2170168.html
