鸭源新型鹅细小病毒DS15株生物学特性研究

发布时间：2018-08-09 16:09

【摘要】：试验对鸭源新型鹅细小病毒(Duck derived goose parvovirus,DDPV)DS15株在鸭胚上的增殖特性及对鸭胚的致病力进行了研究。结果显示,鸭胚接种DDPV DS15株以后,96~120 h出现死亡,胚体全身出血,病毒滴度ELD50为10-5.5/0.2 m L。以鸭胚增殖的病毒攻击樱桃谷肉鸭,复制出典型的鸭"大舌病"临床症状,并从发病鸭脏器中分离到病毒。对DDPVDS15株VP2基因进行克隆并并将其因序列与国内外报道的部分鹅细小病毒(GPV)及番鸭细小病毒(MDPV)分离株的VP2基因进行比对和分析。结果表明,DS15株VP2基因与GPV之间的核苷酸和氨基酸同源性分别为89.2%~96.5%和93.4%~97.8%,而与MDPV核苷酸和氨基酸同源性则较低,分别为80.1%~89.3%和87.8%~93.5%;在亲缘关系方面,DDPV DS15株与GPV较近,提示二者可能是由同一病毒进化而来。
[Abstract]:The proliferative characteristics and pathogenicity of new goose parvovirus (Duck derived goose parvovirus) DS15 strain on duck embryo were studied in this paper. The results showed that the duck embryo died at 96120 h after inoculation with DDPV DS15 strain, and the whole body of the embryo was bleeding, and the virus titer ELD50 was 10-5.5% 0.2 mL. The virus of duck embryo proliferation attacked Cherry Valley meat duck, and the typical clinical symptoms of "big tongue disease" were duplicated, and the virus was isolated from the infected duck organ. The VP2 gene of DDPVDS15 strain was cloned and compared with the VP2 gene of some goose parvovirus (GPV) and Muscovy duck parvovirus (MDPV) isolates reported at home and abroad. The results showed that the nucleotide and amino acid homology between VP2 gene and GPV were 89.22.5% and 93.4%, respectively, but lower with MDPV (80.1%, 89.3%, 87.8%, 93.5%). It suggests that both may have evolved from the same virus.
【作者单位】：安徽农业大学动物科技学院;中国农业科学院上海兽医研究所;甘肃农业大学动物医学院;
【基金】：国家重点研发计划项目(2016YFD0500108) 国家自然科学基金项目(31270194、31502068) 上海市科技兴农重点攻关项目(2016043) 公益性行业(农业)科研专项(201303046) 中央级公益性科研院所基本科研业务费专项(2016JB01)
【分类号】：S852.65

【相似文献】