半番鸭与番鸭精巢组织差异表达转录组测序分析

发布时间：2018-08-12 15:28

【摘要】：【目的】研究半番鸭与番鸭精巢组织转录组差异表达基因,为进一步阐明半番鸭不育的遗传机制奠定理论基础。【方法】利用转录组测序方法对半番鸭和番鸭的精巢组织进行研究,筛选其差异表达基因,并对其功能进行注释和荧光定量PCR(quantitative real-time PCR,QRT-PCR)验证。【结果】测序共获得43.84Gb Clean Data,组装后共获得193 535条Unigene。DESeq分析发现3 597个基因在两个鸭品种间差异表达,其中上调基因1 194个和下调基因2 403个,包括与生殖功能相关的基因,如成纤维细胞生长因子(fibroblast growth factor,FGF)、蛋白激酶A(protein kinase A,PKA)、丝裂原活化蛋白激酶7(mitogen-activated protein kinase 7-like,partial,BMK)、生长因子受体结合蛋白2(growth factor receptor-bound protein 2,GRB2)、c-Jun氨基末端激酶(c-Jun N-terminal kinase,JNK)和肿瘤坏死因子受体超家族成员6(tumor necrosis factor receptor superfamily member 6,FAS)等。GO(gene Ontology)分析发现382个差异基因获得功能注释,其中97个基因涉及发育繁殖生物学过程。KEGG(kyoto Encyclopedia of Genes and Genomes)通路分析表明差异表达基因共富集到50条信号通路中,其中17个通路显著富集,包括丝裂原活化蛋白激酶信号转导通路(mitogen-activated protein kinase signaling pathway,MAPK)、甘油酯代谢(glycerolipid metabolism)以及钙信号途径(calcium signaling pathway)信号通路等,与生殖功能密切相关的有促性腺激素释放激素信号通路(gonadotropin releasing hormone(Gn RH)signaling pathway)和MAPK信号转导通路。经QRT-PCR验证,差异基因表达变化模式与转录组测序结果一致,测序结果可靠。【结论】在转录组水平上筛选出半番鸭和番鸭精巢组织差异表达基因,揭示了Gn RH和MAPK信号通路在鸭的生殖活动中发挥了重要作用,为进一步探索半番鸭生殖系统的分化机理提供可靠的参考依据。
[Abstract]:[objective] to study the differentially expressed genes in testis of muscovy duck and muscovy duck. [methods] the testis of muscovy duck and muscovy duck were studied by transcriptional sequencing method, and the differentially expressed genes were screened. The 43.84Gb Clean data were obtained by sequencing, and a total of 193,535 Unigene.DESeq analysis were obtained. The results showed that 3 597 genes were differentially expressed between two duck breeds. Of these, 1,194 genes were up-regulated and 2,403 down-regulated genes, including genes related to reproductive function. For example, fibroblast growth factor (fibroblast growth), protein kinase A (protein kinase (A (protein kinase), mitogen-activated protein kinase 7-like protein kinase 7 (BMK), growth factor receptor binding protein 2 (growth factor receptor-bound protein 2 (GRB2) c-Jun amino-terminal kinase (c-Jun N-terminal kinase JNK) and tumor necrosis factor receptor superfamily (TNF- receptor superfamily), such as fibroblast growth factor (fibroblast growth), protein kinase (A (protein kinase), mitogen-activated protein kinase 7-like protein kinase (BMK), growth factor receptor binding protein 2 (growth factor receptor-bound protein 2 (GRB2) and tumor necrosis factor receptor (TNF- R) superfamily. Go (gene Ontology) analysis showed that 382 differentially expressed genes were annotated. Among them, 97 genes were involved in developmental reproduction biological process. KEGG (kyoto Encyclopedia of Genes and Genomes) pathway analysis showed that differentially expressed genes were co-enriched into 50 signal pathways, 17 of which were significantly enriched. Mitogen activated protein kinase signal transduction pathway (mitogen-activated protein kinase signaling pathway MAPK), (glycerolipid metabolism) metabolism of glycerol ester and (calcium signaling pathway) signaling pathway of calcium signal pathway, etc. Gonadotropin releasing hormone signaling pathway (gonadotropin releasing hormone (Gn RH) signaling pathway) and MAPK signal transduction pathway are closely related to reproductive function. The results of QRT-PCR showed that the differentially expressed genes were consistent with the results of transcriptome sequencing, and the sequencing results were reliable. [conclusion] the differentially expressed genes in testis of muscovy duck and muscovy duck were screened at the transcriptional level. It was revealed that GnRH and MAPK signaling pathway played an important role in the reproductive activities of duck, which provided a reliable reference for further exploring the differentiation mechanism of muscovy duck reproductive system.
【作者单位】：福建省农业科学院畜牧兽医研究所;福建昌龙集团;福建农林大学食品科学学院;
【基金】：福建省农科院青年人才创新基金(YC2017-7) 福建省省属公益类科研院所基本科研专项(2017R1023-5) 福建省农科院所青年基金(MYQJ2015-5)
【分类号】：S834

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