早期补饲代乳料对哺乳仔猪断奶后腹泻率、结肠菌群及其代谢产物的影响

发布时间：2018-08-12 16:39

【摘要】：旨在研究早期补饲代乳料对哺乳仔猪断奶后腹泻率、结肠菌群及其代谢产物的影响。选取10窝杜×长×大仔猪,随机均分为对照组和试验组,每组5窝。在仔猪出生后第4天开始,对照组仔猪只进行母乳喂养;试验组仔猪在母乳喂养的同时补充代乳料,直到28日龄断奶。断奶后仔猪全部饲喂相同的日粮,在仔猪35日龄时,从对照组和试验组每窝选取1头仔猪屠宰取样。结果表明,与对照组相比,试验组仔猪断奶后一周内腹泻率有下降的趋势(P=0.08),结肠食糜中乳酸杆菌属、梭菌XIVa群的数量显著下降(P0.05),大肠杆菌属有下降的趋势(P=0.05);结肠食糜中乙酸显著增加(P0.05),同时总酸也有增加的趋势(P=0.05);结肠黏膜中白介素-10(IL-10)的基因表达量显著增加(P0.05),同时结肠黏膜中分泌型免疫球蛋白A(s Ig A)浓度显著下降(P0.05)。研究表明,早期补饲代乳料能一定程度上调节结肠内的菌群组成、代谢产物组成以及结肠功能相关的基因表达,有利于机体和肠道的免疫及屏障功能的维持,从而有助于仔猪适应断奶后日粮的转变。
[Abstract]:The aim of this study was to study the effects of early supplemental feeding on diarrhea rate, colonic flora and metabolites of weaning piglets. Ten litters of Duroc 脳 long 脳 large piglets were randomly divided into control group and experimental group with 5 litters in each group. From the 4th day after birth, the piglets in the control group were breast-fed, while the piglets in the experimental group were fed with substitute milk at the same time, until weaning at the age of 28 days. After weaning, all piglets were fed the same diet. At 35 days of age, one piglet was slaughtered and sampled from each litter of the control group and the experimental group. The results showed that, compared with the control group, the diarrhea rate of the piglets in the experimental group decreased within one week after weaning (P0.08), the genus Lactobacillus in the colonic chyme. The number of Clostridium XIVa group decreased significantly (P0.05), the number of Escherichia coli decreased (P0. 05), the acetic acid in colonic chyme increased significantly (P0.05) and the total acid increased (P0. 05); the gene expression of interleukin-10 (IL-10) in colon mucosa increased significantly (P0.05). The concentration of secretory immunoglobulin A (s Ig A) in colon mucosa decreased significantly (P 0.05). The results showed that the early supplementation of milk substitute could regulate the composition of colonic flora, metabolites and the expression of genes related to colon function to a certain extent, which was beneficial to the maintenance of immune and barrier function of body and intestine. It is helpful for piglets to adapt to the change of diet after weaning.
【作者单位】：南京农业大学动物科技学院江苏省消化道营养与动物健康重点实验室消化道微生物研究室;
【基金】：中央高校基本科研业务费专项(KYRC201402)
【分类号】：S828.5

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