槲皮素对注射链脲佐菌素AA肉鸡脂质代谢的作用及机制

发布时间：2018-08-13 13:27

【摘要】：本试验选取1日龄健康、体重相近的爱拔益加(Arbor Acres,AA)肉仔鸡公雏240只,随机分4组,每组6个重复,每个重复10只鸡。适应1周后按50 mg·kg-1·BW剂量连续2次腹腔注射2%链脲佐菌素(streptozotocin,STZ)溶液后,对照组饲喂基础饲粮,槲皮素组分别在基础饲粮中添加0.02%、0.04%、0.06%槲皮素,试验期共6周。本试验通过对肉鸡血糖、血脂以及腹脂沉积进行测定,探讨槲皮素对肉鸡脂质代谢的作用及其机制。1.槲皮素对AA肉鸡生长性能的影响与对照组相比,3水平槲皮素组AA肉鸡的平均日采食量、平均日增重、料重比差异均不显著(P0.05)。2.槲皮素对AA肉鸡屠宰性能的影响与对照组相比,0.04%槲皮素组AA肉鸡腿肌率极显著增加(P0.01),且显著高于0.06%槲皮素组(P0.05);0.04%、0.06%槲皮素组肉鸡腹脂率均显著降低(P0.05,P0.01);3水平槲皮素组屠宰率、半净膛率、全净膛率、胸肌率均无显著差异(P0.05)。3.槲皮素对AA肉鸡血液生化指标的影响与对照组相比,0.04%、0.06%槲皮素组AA肉鸡空腹血糖含量均显著降低(P0.05,P0.01);0.06%槲皮素组肉鸡空腹血清胰岛素浓度显著增加(P0.05);0.04%、0.06%槲皮素组血清总胆固醇含量显著降低(P0.05);0.04%、0.06%槲皮素组血清甘油三酯含量极显著降低(P0.01);3水平槲皮素组血清低密度脂蛋白胆固醇含量均显著降低(P0.05);0.06%槲皮素组血清游离脂肪酸含量显著降低(P0.05);0.02%、0.04%槲皮素组血清TNF-α含量显著降低(P0.05,P0.01);0.04%槲皮素组血清脂联素、瘦素含量均显著升高(P0.01,P0.05)。4.槲皮素对AA肉鸡磷脂酰肌醇-3-激酶/蛋白激酶B(PI3K/PKB)信号通路相关基因表达的影响与对照组相比,0.06%槲皮素组AA肉鸡肝脏胰岛素受体基因表达极显著增加(P0.01);0.04%槲皮素组肝脏PI3K基因表达极显著增加(P0.01);0.04%、0.06%槲皮素组肉鸡肝脏和腿肌PKB基因表达均极显著提高(P0.01);0.06%槲皮素组腿肌胰岛素受体底物-1基因表达极显著增加(P0.01)。综上所述,饲粮中添加一定剂量的槲皮素能刺激胰岛素分泌,降低STZ肉鸡血糖浓度,降低血脂含量,提高瘦素、脂联素的分泌,激活PI3K/PKB信号通路,从而改善脂质代谢,最终降低肉鸡腹脂沉积。
[Abstract]:A total of 240 male broilers of Arbor Acresco AA, who were 1 day old and of similar body weight, were randomly divided into 4 groups with 6 replicates in each group and 10 chickens in each group. After 1 week of adaptation, 2% streptozotocin (STZ) solution was injected intraperitoneally at the dose of 50mg kg-1 BW. The control group was fed with basic diet, and the quercetin group was fed with 0.02% quercetin 0.04% quercetin in the basal diet for 6 weeks. The effects of quercetin on lipid metabolism in broilers and its mechanism were studied by measuring blood glucose, blood lipid and abdominal lipid deposition in broilers. Effects of quercetin on growth performance of AA broilers compared with the control group, the average daily intake, average daily gain and feed weight ratio of AA broilers in Quercetin group were not significantly different (P0.05). Effects of quercetin on slaughter performance of AA broilers compared with the control group, the percentage of leg muscle of AA broilers in 0.04% quercetin group increased significantly (P0.01), and was significantly higher than that in 0.06% quercetin group (P0.05). The percentage of abdominal fat in broilers of 0.04% quercetin group decreased significantly (P0.05P0.01), and the slaughtering rate of quercetin group was significantly higher than that of 0.06% quercetin group (P0.05P0.01). There was no significant difference in the rate of half, total and pectoral muscles (P0.05). Effect of quercetin on blood biochemical indexes of AA broilers compared with control group, the fasting blood glucose content of AA broilers in 0.06% quercetin group was significantly decreased (P0.05, P0.01). The fasting serum insulin concentration in AA broiler chickens in quercetin group was significantly increased (P0.05), and the serum total bile duct in 0.04% quercetin group was significantly higher than that in the control group (P0.05, P0.01). The serum triglyceride content of 0.06% quercetin group was significantly decreased (P0.01) the serum low density lipoprotein cholesterol content in quercetin group was significantly decreased (P0.05) the serum free fatty acid content in 0.06% quercetin group was significantly decreased (P0.05) the serum free fatty acid content in the quercetin group was 0.02% (P0.05) and the content of serum low density lipoprotein cholesterol in quercetin group was 0.04% significantly lower than that in the quercetin group (P0.05). The content of serum TNF- 伪 in quercetin group was significantly lower than that in quercetin group (P0.05, P0.01) and adiponectin in serum of quercetin group was 0.04%. Leptin content increased significantly (P 0.01 P 0.05). 4. Effect of quercetin on expression of phosphatidylinositol -3-kinase / protein kinase B (PI3K/PKB) signaling pathway in AA broilers compared with control group, the expression of insulin receptor gene in liver of AA broiler chickens in 0.06% quercetin group was significantly increased (P0.01) 0.04% quercetin group The expression of PI3K gene in liver and leg muscle of broilers was significantly increased (P0.01). The expression of insulin receptor substrate-1 gene in leg muscle was significantly increased (P0.01) in the group of quercetin 0.04% quercetin (P0.01), and the expression of insulin receptor substrate-1 (P0.01) in muscle of leg muscle in quercetin group was significantly higher than that in group B (P0.01). In conclusion, a certain dose of quercetin in diet can stimulate insulin secretion, reduce blood glucose concentration, reduce blood lipid content, increase the secretion of leptin and adiponectin, activate PI3K/PKB signaling pathway, and improve lipid metabolism in STZ broilers. Ultimately reduce abdominal fat deposition in broilers.
【学位授予单位】：东北农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S831.5

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