骆驼瘤胃内降解含氮杂环化合物细菌的多样性
发布时间：2018-08-13 14:10
【摘要】：【目的】作为典型的荒漠动物,骆驼能够采食其他动物不能够食用的具有强烈气味的或有毒的植物,而不影响其正常生理代谢。研究发现骆驼采食的植物毒素与吡啶、喹啉、吲哚等杂环化合物具有相似的化学结构,所以研究骆驼体内是否存在潜在的杂环化合物降解菌具有重要意义。【方法】本研究采集3头骆驼瘤胃内容物,分别以吡啶、喹啉和吲哚3种含氮杂环化合物为唯一碳源和氮源进行5代富集培养。通过高通量测序技术对瘤胃内容物和5代富集培养细菌进行了测序分析。【结果】骆驼肠道中降解杂环化合物(吡啶、喹啉、吲哚)细菌群体样品中变形菌门、放线菌门、拟杆菌门、浮霉菌门和厚壁菌门等5个门类丰富度最高。骆驼瘤胃内降解吡啶的优势菌可能属于鞘氨醇杆菌属和不动杆菌属,降解吲哚的优势菌主要属于芽孢杆菌属,而降解喹啉的优势菌可能以赖氨酸芽孢杆菌属和鞘氨醇杆菌属为主。【结论】骆驼瘤胃原始样品经过吡啶、喹啉、吲哚富集5代后,与原始样品比较优势菌群发生了较大的改变,这说明骆驼瘤胃内蕴含降解吡啶、吲哚和喹啉的细菌,但对吡啶、吲哚和喹啉的降解过程中发挥降解作用的细菌群落存在差异。
[Abstract]:[objective] as a typical desert animal, camels can feed on plants with strong odor or poison that other animals cannot eat without affecting their normal physiological metabolism. It was found that plant toxins from camels had similar chemical structures to heterocyclic compounds such as pyridine, quinoline, indole, etc. Therefore, it is of great significance to study the existence of potential heterocyclic degrading bacteria in camels. [methods] three camel rumen contents were collected and used as pyridine. Three nitrogen-containing heterocyclic compounds, quinoline and indole, were used as sole carbon and nitrogen sources for 5 generations of enrichment and culture. The rumen contents and 5 generation enriched bacteria were sequenced and analyzed by high throughput sequencing. [results] Proteus and Actinomycetes were degraded in the intestinal tract of camels (pyridine, quinoline, indole). The richness of five phylum, phylum floviformis and phylum platyphylla were the highest. The dominant bacteria for degradation of pyridine in camel's rumen may belong to sphingosine and Acinetobacter, and the dominant bacteria to degrade indole mainly belong to Bacillus. The dominant bacteria for quinoline degradation may be Lysine Bacillus and sphingosine. [conclusion] the original samples of camel rumen were enriched with pyridine, quinoline and indole for 5 generations. Compared with the original sample, the dominant flora changed greatly, which indicated that the camel rumen contains bacteria that degrade pyridine, indole and quinoline, but there are differences in the community of bacteria that play a role in the degradation of pyridine, indole and quinoline in the process of degradation of pyridine, indole and quinoline.
【作者单位】： 新疆师范大学生命科学学院新疆特殊环境物种保护与调控生物学实验室干旱区植物逆境生物学实验室;乌鲁木齐市职业中等专业学校;成都医学院检验医学院;
【基金】：国家自然科学基金(31160028) 新疆师范大学博士科研启动基金(XJNUBS1607) 新疆联合基金(U1503101) 新疆维吾尔自治区自然科学基金(2016D01B049)~~
【分类号】：S824
，
本文编号：2181225
[Abstract]:[objective] as a typical desert animal, camels can feed on plants with strong odor or poison that other animals cannot eat without affecting their normal physiological metabolism. It was found that plant toxins from camels had similar chemical structures to heterocyclic compounds such as pyridine, quinoline, indole, etc. Therefore, it is of great significance to study the existence of potential heterocyclic degrading bacteria in camels. [methods] three camel rumen contents were collected and used as pyridine. Three nitrogen-containing heterocyclic compounds, quinoline and indole, were used as sole carbon and nitrogen sources for 5 generations of enrichment and culture. The rumen contents and 5 generation enriched bacteria were sequenced and analyzed by high throughput sequencing. [results] Proteus and Actinomycetes were degraded in the intestinal tract of camels (pyridine, quinoline, indole). The richness of five phylum, phylum floviformis and phylum platyphylla were the highest. The dominant bacteria for degradation of pyridine in camel's rumen may belong to sphingosine and Acinetobacter, and the dominant bacteria to degrade indole mainly belong to Bacillus. The dominant bacteria for quinoline degradation may be Lysine Bacillus and sphingosine. [conclusion] the original samples of camel rumen were enriched with pyridine, quinoline and indole for 5 generations. Compared with the original sample, the dominant flora changed greatly, which indicated that the camel rumen contains bacteria that degrade pyridine, indole and quinoline, but there are differences in the community of bacteria that play a role in the degradation of pyridine, indole and quinoline in the process of degradation of pyridine, indole and quinoline.
【作者单位】： 新疆师范大学生命科学学院新疆特殊环境物种保护与调控生物学实验室干旱区植物逆境生物学实验室;乌鲁木齐市职业中等专业学校;成都医学院检验医学院;
【基金】：国家自然科学基金(31160028) 新疆师范大学博士科研启动基金(XJNUBS1607) 新疆联合基金(U1503101) 新疆维吾尔自治区自然科学基金(2016D01B049)~~
【分类号】：S824
，
本文编号：2181225
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