bta-miR-205和bta-miR-497调控牛病毒性腹泻病毒复制的研究
发布时间：2018-08-13 14:28
【摘要】：为探究bta-miR-205和bta-miR-497对牛病毒性腹泻病毒(BVDV)复制的调控作用,在前期建立BVDV感染MDBK细胞miRNAs差异表达谱的基础上,筛选出表达量显著差异的bta-miR-205和btamiR-497,通过生物信息学软件预测、荧光素酶报告基因检测等方法鉴定bta-miR-205和bta-miR-497的靶基因。应用RT-qPCR和病毒滴度测定等方法验证bta-miR-205和bta-miR-497对BVDV复制的影响。结果发现,bta-miR-205能显著抑制BVDV的复制,而bta-miR-497显著促进BVDV的复制。研究结果为BVD防控提供了新的思路和依据。
[Abstract]:In order to investigate the regulatory effect of bta-miR-205 and bta-miR-497 on (BVDV) replication of bovine viral diarrhea virus (BVDV), bta-miR-205 and btamiR-497were screened out on the basis of miRNAs differential expression profiles of MDBK cells infected with BVDV in the early stage, and predicted by bioinformatics software. Luciferase reporter gene detection was used to identify the target genes of bta-miR-205 and bta-miR-497. The effects of bta-miR-205 and bta-miR-497 on BVDV replication were tested by RT-qPCR and virus titer assay. The results showed that bta-miR-205 could significantly inhibit the replication of BVDV, while bta-miR-497 significantly promoted the replication of BVDV. The results provide a new idea and basis for the prevention and control of BVD.
【作者单位】： 石河子大学动物科技学院;新疆农业大学动物医学学院;
【基金】：国家自然科学基金项目(U1303283,31502095,31560328) 国际科技合作项目(2013DFR30970)
【分类号】：S852.653
，
本文编号：2181260
[Abstract]:In order to investigate the regulatory effect of bta-miR-205 and bta-miR-497 on (BVDV) replication of bovine viral diarrhea virus (BVDV), bta-miR-205 and btamiR-497were screened out on the basis of miRNAs differential expression profiles of MDBK cells infected with BVDV in the early stage, and predicted by bioinformatics software. Luciferase reporter gene detection was used to identify the target genes of bta-miR-205 and bta-miR-497. The effects of bta-miR-205 and bta-miR-497 on BVDV replication were tested by RT-qPCR and virus titer assay. The results showed that bta-miR-205 could significantly inhibit the replication of BVDV, while bta-miR-497 significantly promoted the replication of BVDV. The results provide a new idea and basis for the prevention and control of BVD.
【作者单位】： 石河子大学动物科技学院;新疆农业大学动物医学学院;
【基金】：国家自然科学基金项目(U1303283,31502095,31560328) 国际科技合作项目(2013DFR30970)
【分类号】：S852.653
，
本文编号：2181260
本文链接：https://www.wllwen.com/yixuelunwen/dongwuyixue/2181260.html
