断奶羔羊4种必需氨基酸限制性顺序和需要量模型探索

发布时间：2018-08-14 11:29

【摘要】：本试验旨在采用氨基酸部分扣除法研究断奶羔羊的4种必需氨基酸的限制性顺序和需要模型。选取50日龄断奶湖羊公羊100只随机分为5组,每组4个重复,每个重复5只羊。对照组为氨基酸平衡(PC)组,饲喂氨基酸平衡的开食料,4个试验组开食料在PC组的基础上分别扣除赖氨酸(PD-Lys组)、蛋氨酸(PD-Met组)、苏氨酸(PD-Thr组)和色氨酸(PD-Trp组),其余氨基酸含量保持一致。预试期为10 d,正试期为60 d。于羔羊60、90和120日龄称量体重,在120日龄每组随机选取6只羔羊进行屠宰。结果表明:1)60~120日龄和90~120日龄,PD-M et组羔羊平均日增重(ADG)显著低于其他各组(P0.05),饲料转化率(F/G)显著高于其他各组(P0.05);120日龄时,PD-Met组宰前活重、空体重、胴体重、眼肌面积、头重、蹄重及皮毛重均显著低于PC、PD-Thr和PD-Trp组(P0.05),但是5组间屠宰率(DP)、GR值和血液重差异不显著(P0.05)。2)以最佳ADG和F/G为评价指标时,60~90日龄和90~120日龄氨基酸限制性顺序为Met、Lys、Thr和Trp;而以最佳DP为评价指标时,120日龄氨基酸的限制性顺序为Trp、Thr、Lys、Met。3)以最佳ADG作为评价指标时,60~90日龄和90~120日龄Lys、M et、Thr和Trp的适宜比例分别为100∶44∶44∶8和100∶42∶38∶12;以最佳F/G作为评价指标时,60~90日龄和90~120日龄Lys、M et、Thr和Trp的适宜比例分别为100∶54∶45∶7和100∶47∶39∶12;以最佳DP为评价指标时,60~120日龄Lys、M et、Thr和Trp的适宜比例为100∶34∶38∶8。总之,饲粮必需氨基酸(Lys、Met、Thr和Trp)缺乏严重影响湖羊断奶羔羊的生长性能、屠宰性能和器官指数,特别是Met影响最为明显,其次是Lys,Thr和Trp影响较弱;不同评价指标(ADG、F/G和DP)和不同生长阶段得出的Lys、M et、Thr和Trp的限制性顺序和需要模型是不同的。
[Abstract]:The aim of this study was to study the restrictive sequence and requirement model of four essential amino acids in weaned lambs by partial amino acid deduction. A total of 100 50-day-old weaned Hu sheep were randomly divided into 5 groups with 4 replicates in each group and 5 sheep in each replica. The control group was amino acid balanced (PC) group. On the basis of PC, lysine (PD-Lys), methionine (PD-Met), threonine (PD-Thr) and tryptophan (PD-Trp) were deducted from the diet of the four experimental groups. The contents of other amino acids remained the same. The pre-test period was 10 days and the positive test period was 60 days. At the age of 6090 and 120 days, 6 lambs were slaughtered randomly in each group of 120 days of age. The results showed that the average daily gain (ADG) of PD-M et group was significantly lower than that of other groups (P0.05), and the feed conversion rate (Fr / G) was significantly higher than that of other groups (P0.05). The body weight, eye muscle area and head weight of PD-Met group were significantly higher than those of other groups (P0.05). The hoof weight and fur weight were significantly lower than those of PCP PD-Thr and PD-Trp groups (P0.05), but there was no significant difference in slaughtering rate (DP) gr and blood weight among the five groups (P0.05). When the best ADG and F / G were used as the evaluation indexes, the restriction order of amino acid was MetLysThr and Trp at 6090 day and 120th day of age respectively; The restrictive sequence of amino acids in the 120-day old of Jia-DP is TrpP-Thrrrr-Lys.3.) the optimum ratio of ADG to Trp is 100: 444444: 8 and 100: 422: 3812, respectively, when the best ADG is used as the evaluation index for 600-day and 120-day-old Lys-Met THR and Trp, respectively, and the optimum ratio is 100: 4444: 8 and 100: 422: 12; the optimum ratio is 100: 4444: 8 and 100: 42: 38: 12, respectively. The suitable ratios of F / G to Trp are 100: 54: 45: 7 and 100: 47: 39: 12, respectively, and the suitable ratio of Trp to Trp is 100: 34: 38: when the best DP is taken as the evaluation index. In a word, the deficiency of dietary essential amino acids (Lyschus Metallothr and Trp) seriously affected the growth performance of weaned lambs, butchery performance and organ index, especially Met, were the most obvious, followed by Lysn Thr and Trp. The restrictive order and need model of Lysmeter Thr and Trp obtained from different evaluation indexes (ADGG / F / G and DP) and different growth stages are different.
【作者单位】：塔里木大学动物科学学院;中国农业科学院饲料研究所农业部饲料生物技术重点开放实验室;
【基金】：农业部公益性行业(农业)科研专项(201303143) 国家肉羊产业技术体系建设专项资金(CARS-39)
【分类号】：S826.5

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