山羊Kiss-1和GPR54基因的克隆及组织表达研究

发布时间：2018-08-14 11:35

【摘要】：为探究Kiss-1和GPR54基因在山羊季节性繁殖中的作用,分别在四川省金堂县和理县各选取5只10月龄处于发情前期的藏山羊和金堂黑山羊为研究对象,对Kiss-1和GPR54基因编码区进行序列及组织表达分析。结果显示:金堂黑山羊和藏山羊Kiss-1基因编码区均长408 bp,编码135个氨基酸,两品种间存在2处同义突变。GPR54基因编码区均长244 bp,编码81个氨基酸,两品种间存在1处同义突变;金堂黑山羊Kiss-1和GPR54基因CDs区核苷酸序列与藏山羊的同源性分别为99.5%、99.6%;Kiss-1和GPR54基因在两品种山羊的卵巢、子宫、输卵管、垂体中均有表达,但只有Kiss-1基因在金堂黑山羊垂体中的表达量显著高于藏山羊(P0.05),而在其他组织品种间差异不显著(P0.05)。提示:Kiss-1基因可能参与调控山羊季节性繁殖,而GPR54基因是否是引起季节性繁殖的原因还有待进一步研究。
[Abstract]:In order to investigate the role of Kiss-1 and GPR54 genes in seasonal reproduction of goats, five Tibetan goats and Jintang black goats, aged 10 months, were selected from Jintang County and Lixian County, Sichuan Province, respectively. The sequence and tissue expression of Kiss-1 and GPR54 gene coding region were analyzed. The results showed that the coding region of Kiss-1 gene in Jintang Black Goat and Tibetan Goat was 408bplong, encoding 135 amino acids. There were 2 synonymous mutations. The coding region of GPR54 gene was 244bp, encoding 81 amino acids, and there was one synonymous mutation between the two breeds. The nucleotide sequence of CDs region of Kiss-1 and GPR54 gene in Jintang Black Goat was 99.55.The nucleotide sequence of Kiss-1 and GPR54 genes were 99.5% and 99.6% respectively in ovary, uterus, fallopian tube and pituitary gland of the two breeds of goats. But only the expression of Kiss-1 gene in pituitary gland of Jintang black goat was significantly higher than that of Tibetan goat (P0.05), but there was no significant difference among other tissue varieties (P0.05). It is suggested that the gene of: Kiss-1 may be involved in the regulation of seasonal reproduction in goats, and whether the GPR54 gene is the cause of seasonal reproduction remains to be further studied.
【作者单位】：西南民族大学生命科学与技术学院;
【基金】：四川省应用基础项目(2013JY0043)
【分类号】：Q78;S827

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