甘南高寒草甸土壤纤毛虫对磷酸氢二铵添加的响应

发布时间：2018-08-19 16:39

【摘要】：为研究磷酸氢二铵(DAP)添加对土壤纤毛虫群落结构的影响,于2012年4月在甘南高寒草甸选取典型样地,在0~120 g·m~(-2)浓度区间内设置5个添加水平,采用"非淹没培养皿法",活体观察法和"3级10倍环式稀释法"测定土壤纤毛虫物种数和密度,同时测定了土壤相关环境因子(含水量、pH值、总氮、总磷、有机碳).共获得土壤纤毛虫129种,隶属9纲17目31科46属.对照组的土壤纤毛虫物种数和物种多样性指数均大于施肥处理,且随着施肥量的增大,土壤纤毛虫物种数减少,物种多样性减小,但其密度随施肥量的增大而增加.对照土壤纤毛虫优势类群为刺钩目,随着施肥量的增大,土壤纤毛虫优势类群更新为肾形目.土壤纤毛虫群落结构和环境因子的冗余分析表明,总磷、土壤温度和含水量是影响甘南高寒草甸土壤纤毛虫群落分布的关键环境因子.
[Abstract]:In order to study the effect of (DAP) addition on the community structure of soil ciliates, a typical plot was selected in Gannan alpine meadow in April 2012, and five supplementation levels were set up in the concentration range of 0 ~ 120g m ~ (-2) in Gannan Alpine Meadow. The species number and density of soil ciliates were determined by "non-submerged petri dish method", "living observation method" and "3-grade 10-fold ring dilution method", and the soil related environmental factors (pH value of water content, total nitrogen, total phosphorus, organic carbon) were determined at the same time. A total of 129 species of soil ciliates belonging to 9 classes, 17 orders, 31 families and 46 genera were obtained. The species number and species diversity index of soil ciliates in control group were higher than that of fertilization treatment, and with the increase of fertilizer amount, the species number and species diversity of soil ciliates decreased, but the density increased with the increase of fertilization. The dominant group of soil ciliates in the control group was the order Ciliformis. With the increase of fertilization amount, the dominant group of soil ciliates was replaced by the order of kidney. Redundancy analysis of community structure and environmental factors of soil ciliates showed that total phosphorus, soil temperature and water content were the key environmental factors affecting the distribution of soil ciliates in alpine meadow of Gannan.
【作者单位】：西北师范大学生命科学学院;兰州大学生命科学学院;
【基金】：国家自然科学基金项目(41361055,40930533)资助~~
【分类号】：S812.2

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