不同剂量酪酸菌对脂多糖攻毒白羽肉鸡肠道形态及免疫因子mRNA表达的影响

发布时间：2018-08-21 15:03

【摘要】：本试验旨在考察不同剂量酪酸菌对脂多糖(LPS)攻毒白羽肉鸡肠道形态及免疫因子m RNA表达的影响。试验将240只7日龄白羽肉鸡随机分为4个处理组,负对照组和正对照组的饮水和日粮中不添加任何微生态制剂,试验1组和试验2组分别在饮水中添加5×104、5×10~3 CFU/m L酪酸菌。21日龄时每个处理组随机选取3只鸡,正对照组和2个试验组采用腹腔注射的方式注射5 mg/kg LPS,负对照组腹腔注射等量的生理盐水。结果表明:LPS显著提高了致炎因子IL-1β和IL-6的m RNA表达水平(P0.05),且TLR-5的m RNA表达水平也显著升高(P0.05);而酪酸菌显著降低了IL-1β、IL-6和TLR-5水平(P0.05),并显著提高了空肠绒毛高度及绒毛高度/隐窝深度(P0.05),保护了肠道的屏障功能。本试验中添加高剂量酪酸菌的试验组抑炎效果最佳,并显著降低了白羽肉鸡的耗料增重比(P0.05),改善了生长前期肉鸡的生长性能。
[Abstract]:The purpose of this study was to investigate the effects of different doses of casein on intestinal morphology and expression of immune factor m RNA in lipopolysaccharide (LPS) infected white feather broilers. 240 7-day-old white broilers were randomly divided into four treatment groups. The drinking water and diet of negative control group and positive control group were not supplemented with any microecological agents. At the age of 21 days, three chickens were randomly selected from each treatment group, which were supplemented with 5 脳 10 4 ~ 5 脳 10 ~ (-3) CFU/m / L butyric acid bacteria in drinking water of group 1 and group 2, respectively. The positive control group and two experimental groups were injected intraperitoneally with 5 mg/kg LPS, while the negative control group received the same amount of normal saline. The results showed that the expression of m RNA of IL-1 尾 and IL-6 increased significantly (P0.05), and the expression of m RNA of TLR-5 increased significantly (P0.05), while the levels of IL-1 尾 IL-6 and TLR-5 decreased significantly (P0.05), and the height of jejunum villi and chorionic villi increased significantly (P0.05). Height / recess depth (P0.05) protects intestinal barrier function. In this experiment, the experimental group with high dose of butyric acid bacteria had the best anti-inflammatory effect, and significantly reduced the feed consumption and weight gain ratio (P0.05) of white feather broilers, and improved the growth performance of broilers in the early stage of growth.
【作者单位】：青岛根源生物集团技术支持中心;
【分类号】：S831.5

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