影响猪树突状细胞对猪细小病毒样颗粒提呈方式的因素
发布时间：2018-08-25 12:29
【摘要】：旨在探明猪细小病毒样颗粒(PPV-VLPs)被猪脾树突状细胞(DC)捕获后,被提呈的方式。首先通过磁性筛选的方法从非免疫猪和免疫猪的脾分离CD172a+CD11R+DC及CD4-CD8+T细胞。DC分别与伯氨喹、放线菌酮、氯喹、乳胞素、布雷菲德菌素A及亮抑肽酶、胃酶抑素等作用1h后,再与细小病毒样颗粒PPV-VLPs-E290在37℃作用4h,应用CD8+T细胞的细胞毒性分析检测DC对PPV-VLPs-E290的提呈情况。乳酸脱氢酶(LDH)释放试验检测结果显示,伯氨喹及亮抑肽酶对DC提呈PPV-VLPs-E290的效率无明显影响,胃酶抑素部分抑制,而氯喹、放线菌酮、布雷菲德菌素A及乳胞素则可使DC提呈PPV-VLPs-E290的效率明显下降。结果表明,DC通过交叉提呈的方式提呈PPV-VLPs-E290。晚期内体的酸性环境以及蛋白酶的水解均参与了PPV-VLPs-E290的提呈过程。
[Abstract]:The aim of this study was to identify the way in which porcine parvovirus like particles (PPV-VLPs) were captured by porcine splenic dendritic cells (DC). Firstly, CD172a CD11R DC and CD4-CD8 T cells were isolated from the spleen of non-immunized pigs and immunized pigs by magnetic screening method. The cells were treated with primary aminoquine, actinomycin, chloroquine, lactocytin, Blefedgenin A, brilliant aprotinin and gastric endostatin for 1 h, respectively. After being exposed to parvovirus like particle PPV-VLPs-E290 for 4 h at 37 鈩，
本文编号：2202889
[Abstract]:The aim of this study was to identify the way in which porcine parvovirus like particles (PPV-VLPs) were captured by porcine splenic dendritic cells (DC). Firstly, CD172a CD11R DC and CD4-CD8 T cells were isolated from the spleen of non-immunized pigs and immunized pigs by magnetic screening method. The cells were treated with primary aminoquine, actinomycin, chloroquine, lactocytin, Blefedgenin A, brilliant aprotinin and gastric endostatin for 1 h, respectively. After being exposed to parvovirus like particle PPV-VLPs-E290 for 4 h at 37 鈩，
本文编号：2202889
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