胍基乙酸的致突变性研究

发布时间：2018-08-25 14:34

【摘要】：本文选用Ames试验、小鼠骨髓细胞微核试验和精子畸形试验三种短期测试组合探究胍基乙酸是否具有致突变作用。结果发现:Ames试验结果显示,胍基乙酸在8~5000μg/皿,有或无代谢活化系统(S9)时,对鼠伤寒沙门氏菌TA_(97)、TA_(98)、TA_(100)、TA_(102)四种试验株均没有诱变性;骨髓细胞微核试验结果显示,胍基乙酸在1250~5000 mg/kg体重剂量内经口染毒后,各剂量组小鼠骨髓细胞中嗜多染红细胞(PCE)微核率与阴性对照组比较均无显著性差异(P0.05);小鼠精子畸形试验结果显示,胍基乙酸在1250~5000 mg/kg体重剂量内经口染毒后,各剂量组小鼠精子畸形率与阴性对照组比较均无显著性差异(P0.05)。结果表明,三个致突变试验结果均为阴性,胍基乙酸为非致突变物质。
[Abstract]:In this paper, Ames test, mouse bone marrow micronucleus test and sperm malformation test were used to study the mutagenicity of guanidoacetic acid. The results showed that guanidinoacetic acid had no mutagenicity on TA_ _ (97) Tap _ (100) TA102.The results of micronucleus test of bone marrow cells showed that there was no mutagenicity in the presence or absence of metabolic activation system (S9) for guanidinoacetic acid (GAA) in the presence or absence of metabolic activation system (S9), and the results of micronucleus test in bone marrow cells showed that there was no mutagenicity in the four test strains of Salmonella typhimurium, There was no significant difference in micronucleus rate of polychromatic erythrocyte (PCE) in bone marrow cells between the two groups after oral administration of guanidine acetic acid at the dose of 1250,5000 mg/kg (P0.05), and the results of sperm malformation test showed that the micronucleus rate of polychromatic erythrocytes in the bone marrow cells of the mice was higher than that of the control group (P0.05). There was no significant difference in sperm deformity rate between each dose group and negative control group after oral administration of guanidine acetic acid at 1250 mg/kg body weight (P0.05). The results showed that all the three mutagenicity tests were negative and guanidine acetic acid was a non-mutagenic substance.
【作者单位】：中国农业大学农业部兽药安全监督检验测试中心(北京);固安君德同创生物工程有限公司;北京君德同创农牧科技股份有限公司;
【基金】：北京市科技计划项目(Z151100001215001)
【分类号】：S816

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