不同遗传背景猪群体黏病毒耐药蛋白1基因多态性分析
发布时间：2018-08-25 15:46
【摘要】：为探讨不同遗传背景猪黏病毒耐药蛋白1(myxovirus resistance,Mx1)基因的遗传多态性,本试验采用RT-PCR方法扩增克隆出猪Mx1基因并进行生物信息学分析,采用高分辨率熔解曲线法(HRM)对民猪、长白山野猪与民猪杂交猪(简称野杂猪)和大白猪3个群体Mx1基因外显子2区多态性进行分析。结果显示,从民猪及野杂猪中成功克隆出4条Mx1基因mRNA序列。该序列含有1个1 992bp的完整开放阅读框(ORF),编码663个氨基酸。比较发现4条Mx1基因序列中共含有30个SNPs位点,其中17个SNPs位点引起了氨基酸序列的变异。功能结构域分析发现,大部分氨基酸突变位点位于Mx1基因功能结构域两侧。HRM多态性分析显示,Mx1基因外显子2区DD基因型作为优势基因型在3个群体中具有最高的基因型频率,其中大白猪中DD基因型频率为100%,野杂猪为62.5%,民猪为56.2%。基因多态性分析还发现该区域存在更多的核苷酸及氨基酸突变位点,表明民猪和野杂猪Mx1基因存在更丰富的基因多态性。
[Abstract]:In order to investigate the genetic polymorphism of porcine myxovirus resistance protein 1 (myxovirus resistance,Mx1) gene from different genetic backgrounds, porcine Mx1 gene was amplified by RT-PCR method and analyzed by bioinformatics. High resolution fusion curve method (HRM) was used to analyze the genetic polymorphism of porcine myxovirus resistance protein 1 (myxovirus resistance,Mx1) gene. The polymorphism of exon 2 of Mx1 gene in three populations of Changbai Mountain wild boar and Min pig (wild hybrid pig) and big white pig was analyzed. The results showed that four mRNA sequences of Mx1 gene were cloned successfully from Min pig and wild crossbred pig. The sequence contains a 1 992bp complete open reading frame (ORF), encoding 663 amino acids. A total of 30 SNPs loci were found in 4 Mx1 gene sequences, 17 of which caused amino acid sequence variation. The results of functional domain analysis showed that most of the amino acid mutation loci were located at the two sides of the Mx1 gene functional domain. The results showed that the DD genotype of exon 2 of Mx1 gene had the highest genotype frequency among the three populations. The frequency of DD genotypes in large white pigs, wild crossbred pigs and Min pigs were 100, 62.5 and 56.2, respectively. It was also found that there were more nucleotide and amino acid mutation sites in this region, which indicated that the Mx1 gene of Minzhou pig and wild crossbred pig had more polymorphism.
【作者单位】： 吉林省农业科学院畜牧分院;延边大学农学院;
【基金】：吉林省重点科技公关项目(20140204061NY)
【分类号】：S828
，
本文编号：2203377
[Abstract]:In order to investigate the genetic polymorphism of porcine myxovirus resistance protein 1 (myxovirus resistance,Mx1) gene from different genetic backgrounds, porcine Mx1 gene was amplified by RT-PCR method and analyzed by bioinformatics. High resolution fusion curve method (HRM) was used to analyze the genetic polymorphism of porcine myxovirus resistance protein 1 (myxovirus resistance,Mx1) gene. The polymorphism of exon 2 of Mx1 gene in three populations of Changbai Mountain wild boar and Min pig (wild hybrid pig) and big white pig was analyzed. The results showed that four mRNA sequences of Mx1 gene were cloned successfully from Min pig and wild crossbred pig. The sequence contains a 1 992bp complete open reading frame (ORF), encoding 663 amino acids. A total of 30 SNPs loci were found in 4 Mx1 gene sequences, 17 of which caused amino acid sequence variation. The results of functional domain analysis showed that most of the amino acid mutation loci were located at the two sides of the Mx1 gene functional domain. The results showed that the DD genotype of exon 2 of Mx1 gene had the highest genotype frequency among the three populations. The frequency of DD genotypes in large white pigs, wild crossbred pigs and Min pigs were 100, 62.5 and 56.2, respectively. It was also found that there were more nucleotide and amino acid mutation sites in this region, which indicated that the Mx1 gene of Minzhou pig and wild crossbred pig had more polymorphism.
【作者单位】： 吉林省农业科学院畜牧分院;延边大学农学院;
【基金】：吉林省重点科技公关项目(20140204061NY)
【分类号】：S828
，
本文编号：2203377
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