日粮添加灰毡毛忍冬藤叶粉对肥育猪血清生化指标与脂肪代谢的影响
发布时间：2018-08-25 18:56
【摘要】：本试验旨在研究日粮中添加灰毡毛忍冬藤叶粉对肥育猪血清生化、肝脏中脂肪酸组成以及脂肪代谢相关基因表达的影响。选用同栏舍、同批次的二元杂交(长×大)健康阉公猪(55±1.03 kg)16栏,按照体重相近原则随机分成2个处理组,即基础日粮(对照组)、基础日粮+1%灰毡毛忍冬藤叶粉,每组8个重复,每个重复10头。试验期为60 d。结果表明:1)与对照组相比,日粮添加灰毡毛忍冬藤叶粉降低了血清中总胆固醇(TC)(P0.05),而显著提高C型反应蛋白(CRP)含量(P0.05);2)与对照组相比,日粮中添加灰毡毛忍冬藤叶粉使肝脏中饱和脂肪酸(SFA)中c13:0和单不饱和脂肪酸(MUFA)中的c14:1含量均显著降低(P0.05);3)与对照组相比,日粮中添加灰毡毛忍冬藤叶粉上调了肝脏中甘油三酯脂肪酶(ATGL)和肉碱棕榈酰基转移酶1(CPT1A)的基因表达量(P0.05);而下调了脂肪合成酶(FAS)的基因表达量(P0.10)。综上所述,日粮中添加灰毡毛忍冬藤叶粉能有效降低肥育猪血液中胆固醇和甘油三酯的含量,可能通过调控脂肪代谢基因ATGL、CPT1和FAS的表达,进而调控育肥猪肝脏的脂肪代谢。
[Abstract]:The purpose of this study was to study the effects of the addition of leaf powder of Lonicera japonica on serum biochemistry, fatty acid composition in liver and the expression of genes related to fat metabolism in fattening pigs. In the same column and in the same batch, 16 rows of crossbred (long 脳 large) healthy castrated boars (55 卤1.03 kg) were randomly divided into two groups according to the principle of similar body weight: basal diet (control group) and basal diet of 1% powdered leaves of Lonicera feldis. There were 8 replicates in each group and 10 repeats in each group. The experimental period is 60 days. The results showed that compared with the control group, the content of serum total cholesterol (TC) (P0.05) was significantly increased in the diet supplemented with the leaf powder of Lonicera felvidis (P0.05), but the content of C-type reactive protein (CRP) was significantly increased (P0.05) compared with the control group. The contents of C13: 0 in (SFA) and C14: 1 in monounsaturated fatty acid (MUFA) in liver were significantly decreased (P0.05) compared with the control group. The gene expression of triglyceride lipase (ATGL) and carnitine palm acyltransferase 1 (CPT1A) in liver was up-regulated by adding leaf powder of Lonicera japonica in diet (P0.05), but the gene expression of (FAS) was down-regulated (P0.10). To sum up, the dietary supplementation of leaf powder of Lonicera feldis can effectively reduce the content of cholesterol and triglyceride in blood of fattening pigs, which may regulate the fat metabolism of the liver of fattening pigs by regulating the expression of fat metabolism genes ATGL,CPT1 and FAS.
【作者单位】： 湖南农业大学湖南畜禽安全生产协同创新中心;中国科学院亚热带农业生态研究所中国科学院亚热带农业生态过程重点实验室湖南省畜禽健康养殖工程技术研究中心;
【基金】：湖南省重大专项(2015NK1002) “十二五”农村领域国家科技计划(2012BAD39B03) 湖南省面上项目(S2014J504I) 湖南省重点研发计划(2016YFD0501209)
【分类号】：S828.5
，
本文编号：2203801
[Abstract]:The purpose of this study was to study the effects of the addition of leaf powder of Lonicera japonica on serum biochemistry, fatty acid composition in liver and the expression of genes related to fat metabolism in fattening pigs. In the same column and in the same batch, 16 rows of crossbred (long 脳 large) healthy castrated boars (55 卤1.03 kg) were randomly divided into two groups according to the principle of similar body weight: basal diet (control group) and basal diet of 1% powdered leaves of Lonicera feldis. There were 8 replicates in each group and 10 repeats in each group. The experimental period is 60 days. The results showed that compared with the control group, the content of serum total cholesterol (TC) (P0.05) was significantly increased in the diet supplemented with the leaf powder of Lonicera felvidis (P0.05), but the content of C-type reactive protein (CRP) was significantly increased (P0.05) compared with the control group. The contents of C13: 0 in (SFA) and C14: 1 in monounsaturated fatty acid (MUFA) in liver were significantly decreased (P0.05) compared with the control group. The gene expression of triglyceride lipase (ATGL) and carnitine palm acyltransferase 1 (CPT1A) in liver was up-regulated by adding leaf powder of Lonicera japonica in diet (P0.05), but the gene expression of (FAS) was down-regulated (P0.10). To sum up, the dietary supplementation of leaf powder of Lonicera feldis can effectively reduce the content of cholesterol and triglyceride in blood of fattening pigs, which may regulate the fat metabolism of the liver of fattening pigs by regulating the expression of fat metabolism genes ATGL,CPT1 and FAS.
【作者单位】： 湖南农业大学湖南畜禽安全生产协同创新中心;中国科学院亚热带农业生态研究所中国科学院亚热带农业生态过程重点实验室湖南省畜禽健康养殖工程技术研究中心;
【基金】：湖南省重大专项(2015NK1002) “十二五”农村领域国家科技计划(2012BAD39B03) 湖南省面上项目(S2014J504I) 湖南省重点研发计划(2016YFD0501209)
【分类号】：S828.5
，
本文编号：2203801
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