阿魏根粗多糖对鸡脾脏和外周血淋巴细胞体外增殖作用的影响

发布时间：2018-08-27 09:19

【摘要】：为研究新疆阿魏多糖(FSP)对鸡外周血T淋巴细胞和脾脏B淋巴细胞增殖作用的影响。本研究用水提醇沉法得到阿魏总多糖(FSPt)和3种分级多糖(FSP30、FSP50、FSP70),并通过苯酚—硫酸法和红外光谱对FSP进行检测。通过MTT法测定各级FSP的安全浓度及其对鸡外周血T淋巴细胞及脾脏B淋巴细胞的增殖能力。结果显示,提取的FSP均具有多糖的特征峰,为多糖类化合物。外周血T淋巴细胞增殖试验表明,除FSP50外,所有多糖在一定浓度下均能单独和协同PHA刺激T淋巴细胞增殖,其中FSPt的增殖作用较强。脾脏B淋巴细胞增殖试验表明,除FSP50外,所有多糖在一定浓度下均能单独和协同LPS刺激B淋巴细胞增殖,其中FSP70和FSPt增殖作用较强。综上所述,各级FSP中,FSPt对脾脏B淋巴细胞和外周血T淋巴细胞的增殖作用最强,可以作为进一步研究的材料。
[Abstract]:To study the effect of Xinjiang Ferula polysaccharide (FSP) on the proliferation of T lymphocytes and B lymphocytes in chicken peripheral blood. In this study, the total polysaccharide of ferulic (FSPt) and three kinds of graded polysaccharides (FSP30,FSP50,FSP70) were obtained by water extraction and alcohol precipitation, and FSP was determined by phenol-sulfuric acid method and infrared spectrum. The safe concentration of FSP at all levels and its proliferation ability to T lymphocytes and B lymphocytes in peripheral blood of chicken were determined by MTT method. The results showed that the extracted FSP had the characteristic peak of polysaccharides and was a polysaccharide compound. The T lymphocyte proliferation test in peripheral blood showed that all polysaccharides except FSP50 could stimulate T lymphocyte proliferation alone and in coordination with PHA, and FSPt had stronger proliferative effect. Splenic B lymphocyte proliferation test showed that all polysaccharides except FSP50 could stimulate B lymphocyte proliferation alone and in coordination with LPS. FSP70 and FSPt had stronger proliferative effects. In conclusion, the proliferation of spleen B lymphocytes and peripheral blood T lymphocytes by FSPt was the strongest in all levels of FSP, and could be used as a material for further study.
【作者单位】：新疆农业大学动物医学学院;
【基金】：国家自然科学基金项目(31360625) 新疆农业大学研究生创新项目(XJAUGRI2015015)
【分类号】：S831.5

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