番鸭A-FABP基因单核苷酸多态与生产性状的相关性研究

发布时间：2018-08-27 19:10

【摘要】：为了解A-FABP基因单核苷酸多态在番鸭群体中的分布情况,以及单核苷酸多态与生产性能的关联性,采用PCR-SSCP技术对番鸭A-FABP基因进行多态性研究,分析该基因与体重、屠宰性能、常规肉品质的相关性。结果表明:番鸭A-FABP基因上发现A-C突变,位于第2内含子第201处碱基,形成了AA、AB、BB3种基因型,A等位基因频率为0.8241,B等位基因频率为0.175 9,群体纯合度为0.710 0,多态性信息含量为0.247 9。除第2、4周龄外,AA型公鸭早期体重显著高于AB型公鸭,母鸭不同基因型之间无显著差异。公鸭的宰前活重、屠体重、半净膛重、全净膛重、胸肌重、腹脂重、腹脂率指标在不同基因型之间存在显著性差异,AB型均小于AA型。母鸭只有腹脂重、腹脂率在不同基因型间存在显著性差异,AB型低于AA型。番鸭AFABP基因第2内含子A201C单核苷酸突变对腹脂沉积、公鸭体重有显著影响。
[Abstract]:In order to understand the distribution of single nucleotide polymorphism of A-FABP gene in Muscovy duck population and the relationship between single nucleotide polymorphism and production performance, PCR-SSCP technique was used to study the polymorphism of A-FABP gene in muscovy duck. Correlation of conventional meat quality. The results showed that A-C mutation was found in the A-FABP gene of Muscovy duck, which was located at the second intron 201. The frequency of allele A was 0.175 9, the homozygosity of population was 0.710 0, and the information content of polymorphism was 0.247 9. The early body weight of AA type male duck was significantly higher than that of AB type male duck except for the 2nd week old, but there was no significant difference among different genotypes of female duck. There were significant differences in pre-slaughter weight, carcass weight, half-weight, full weight, breast muscle weight and abdominal fat rate between male ducks and male ducks compared with AA genotype. There was significant difference in abdominal fat rate between different genotypes and AB type was lower than AA type. The single nucleotide mutation in intron 2 of muscovy duck AFABP gene (A201C) had a significant effect on abdominal fat deposition and body weight of male ducks.
【作者单位】：江苏农牧科技职业学院;
【基金】：江苏省高校“青蓝工程”项目(苏教师【2016】15号) 江苏省农业科技自主创新资金项目[CX(14)2072] 江苏农牧科技职业学院院级课题(NSF201601)
【分类号】：S834

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