饲粮添加脯氨酸对环江香猪繁殖性能及其后代生长发育的影响
发布时间：2018-08-28 19:35
【摘要】：为了探讨饲粮添加脯氨酸对妊娠环江香猪繁殖性能及其后代生长发育的影响,本论文选取48头体重约25 Kg的后备环江香猪母猪,自然交配,配种15 d后随机分成3组,每组8个重复(栏),每个重复2头猪。分别饲喂添加0.77%L-丙氨酸(等氮对照)、1.00%L-脯氨酸、0.77%L-丙氨酸+0.0167%二氟甲基鸟氨酸(DFMO,多胺合成抑制剂)的试验饲粮。分别于配种第45和70 d,每栏选取1头母猪,放血处死,收集血液,分离血浆,用于生化参数、游离氨基酸和多胺分析;解剖母猪,取出整个子宫,分离并称取孕体重,记录黄体数;分别收集羊水和尿囊液,逐一剥离胎猪,称取胎猪个体重并记录胎儿数;丙氨酸组每头母猪分别选取1头最大、接近平均和最小体重胎猪的羊水和尿囊液,其余2组每头母猪选取1头接近平均体重胎猪的羊水和尿囊液,用于生化参数和游离氨基酸分析。另外选取15头体重约25 Kg的后备环江香猪母猪,自然交配,配种15 d后随机分成3组,每组5个重复(栏),每个重复1头猪。日粮处理同前。自然分娩后2 h内记录仔猪头数并称重,选取最大、接近平均和最小体重仔猪另用于研究脯氨酸对初生仔猪肠道功能发育的影响,剩余仔猪由母猪哺乳喂养,分别于7、14日龄每窝随机选取1头仔猪,称重后放血处死,收集血液,分离血浆,用于生化参数、游离氨基酸和多胺分析;解剖仔猪,分离心脏、肝脏、肾脏和脾脏并称重,计算内脏器官系数。试验结果表明:1、饲粮添加脯氨酸对妊娠环江香猪繁殖性能和血浆化学成分的影响。妊娠第70 d时,脯氨酸组窝重显著高于丙氨酸组(P0.05),其余试验各组的黄体数、胎儿数、窝重、孕体重和胎儿重差异不显著(P0.05)。妊娠第45 d时,脯氨酸组血浆谷丙转氨酶活性和乳酸浓度均显著低于丙氨酸组(P0.05),其他指标各组间均无显著差异(P0.05)。妊娠第70 d与45 d相比,丙氨酸组和DFMO组血浆白蛋白浓度、脯氨酸组和DFMO组血浆总胆固醇和腐胺浓度以及各处理组血浆谷氨酰胺浓度均显著降低、3-甲基组氨酸浓度显著升高(P0.05)。2、饲粮添加脯氨酸对妊娠环江香猪羊水和尿囊液化学成分的影响。妊娠第45和70 d时,各处理组胎儿重和孕体重均无显著差异(P0.05)。妊娠第45 d时,DFMO组羊水天冬氨酸、谷氨酸、异亮氨酸和精氨酸浓度均显著高于丙氨酸组(P0.05);妊娠第70 d时,脯氨酸组羊水半胱氨酸浓度显著高于、尿囊液白蛋白和精氨酸浓度均显著低于丙氨酸组(P0.05);其余指标各组间均无显著差异(P0.05)。妊娠第70 d与45 d相比,各处理组羊水谷草转氨酶活性显著升高(P0.05)、苯丙氨酸和异亮氨酸以及丝氨酸浓度均显著降低(P0.05),丙氨酸组和脯氨酸组羊水γ-谷氨酰转肽酶活性显著升高、赖氨酸浓度显著降低(P0.05),丙氨酸组和DFMO组羊水葡萄糖浓度显著升高、亮氨酸和半胱氨酸浓度均显著降低(P0.05),脯氨酸组和DFMO组羊水乳酸脱氢酶活性和尿素氮浓度均显著升高、精氨酸浓度显著降低(P0.05);各处理组尿囊液尿素氮、尿酸和肌酐浓度均显著升高(P0.05),脯氨酸组和DFMO组尿囊液谷氨酸和精氨酸浓度均显著降低(P0.05)。3、母猪饲粮添加脯氨酸对环江香猪哺乳仔猪血浆化学成分的影响。各处理组初生仔猪数和初生体重间均无显著差异(P0.05);7日龄时,脯氨酸组和DFMO组仔猪血氨浓度显著低于丙氨酸组(P0.05);14日龄时,脯氨酸组仔猪体重显著高于其他两组(P0.05),仔猪血浆葡萄糖、精氨酸和脯氨酸浓度均显著高于丙氨酸组(P0.05);DFMO组仔猪血浆血氨和葡萄糖浓度均显著低于脯氨酸组(P0.05),精氨酸、鸟氨酸和天冬酰胺浓度均显著高于丙氨酸组(P0.05),天冬酰胺浓度显著高于脯氨酸组(P0.05)。4、环江香猪不同体重胎儿对应羊水和尿囊液化学成分的差异。妊娠第70 d时,最小体重组胎儿重显著低于最大体重和中间体重组(P0.05)。妊娠第45 d时,最小体重组胎儿对应尿囊液谷丙转氨酶、谷草转氨酶和乳酸脱氢酶活性以及白蛋白、乳酸、葡萄糖和甘油三酯浓度均显著低于中间体重组(P0.05);最大体重组胎儿对应羊水精氨酸浓度显著高于中间体重组(P0.05)、尿囊液乳酸浓度显著低于中间体重组(P0.05)。妊娠第70 d时,最大体重组胎儿对应羊水乳酸脱氢酶活性显著高于中间体重组(P0.05),最小体重组胎儿对应羊水肌酐和甘油三酯浓度均显著高于最大体重组(P0.05)。综上所述,通过饲粮添加脯氨酸或DMFO可改变妊娠环江香猪及其后代的营养代谢状况,从而影响母猪繁殖性能及其后代的生长发育特性;胎儿体重大小与羊水和尿囊液中部分营养素及其代谢物的含量密切相关。
[Abstract]:In order to investigate the effects of dietary proline supplementation on reproductive performance and offspring growth of Huanjiang Xiang pig, 48 * * * Huanjiang sow sows with a weight of about 25 Kg were selected. They were naturally copulatory and 15 d after mating. They were randomly divided into 3 groups, each containing 8 replicates (columns) and 2 pigs per replicate. Feeding 0.77%L- alanine (equal nitrogen contrast), 1, respectively. 0% L-proline, 0.77% L-alanine + 0.0167% difluoromethyl ornithine (DFMO, polyamine synthesis inhibitor) test diet. At the 45th and 70th day of mating, one sow in each column was selected, bled and killed, blood was collected, plasma was separated for biochemical parameters, free amino acids and polyamines analysis; dissected sows, removed the whole uterus, and separated and weighed pregnancy weight. The number of corpus luteum was recorded, the amniotic fluid and allantoic fluid were collected, the * * * the fetal pig was stripped one by one, the weight of the fetal pig was recorded and the number of fetuses was recorded; the alanine group was selected with 1 largest, near average and minimum weight fetal water and amniotic fluid respectively, and the other 2 groups of 1 sows and the allantoic fluid which were close to the average weight of the pigs were used for biochemical analysis. Analysis of parameters and free amino acids. In addition, 15 Huanjiang sows with a weight of about 25 Kg were selected and naturally bred * * *. After mating 15 d, they were randomly divided into 3 groups. Each group had 5 replicates (columns) and 1 pigs per replicate. The diet was treated with the same method. The number of piglets and the weight of piglets were recorded in 2 h after natural childbirth, and the largest, near average and minimum weight piglets were used for other purposes. To study the effect of proline on intestinal function and development of newborn piglets, the remaining piglets were fed by suckling sows. One piglet was randomly selected at the age of 7 and 14 days. After weighing, one piglet was killed. Blood was collected and plasma was separated for biochemical parameters, free amino acids and polyamines analysis. The results showed that: 1. The effect of proline supplementation on reproductive performance and plasma chemical composition of pregnant Huanjiang Xiang pig. The litter weight of proline group was significantly higher than that of alanine group on the seventieth day of pregnancy (P 0.05). The number of corpus luteum, fetal number, litter weight, pregnancy weight and fetal weight of other experimental groups were not significantly different (P 0.05). The plasma glutamate-alanine transaminase activity and lactate concentration in proline group were significantly lower than those in alanine group (P Amide concentration decreased significantly, 3-methylhistidine concentration increased significantly (P 0.05). 2. Effects of dietary proline supplementation on amniotic fluid and allantoic liquefaction chemical composition of pregnant Huanjiang Xiang pig. Fetal weight and gestational weight of each treatment group had no significant difference on the 45th and 70th days of pregnancy (P 0.05). At the 45th day of pregnancy, aspartic acid, glutamate and isoleucine in amniotic fluid of DFMO group were not significantly different. The concentrations of acid and arginine were significantly higher than those of alanine group (P 0.05); the concentrations of amniotic fluid cysteine, allantoic albumin and arginine in proline group were significantly higher than those in alanine group on the seventieth day of pregnancy (P 0.05); the other indicators were not significantly different between groups (P 0.05). Compared with the seventieth and forty-five days of pregnancy, the amniotic fluid oxaloacetic acid transamination in each treatment group was significantly higher. Amniotic fluid gamma-glutamyl transpeptidase activity and lysine concentration in alanine group and proline group increased significantly (P 0.05). Amniotic fluid glucose concentration in alanine group and DFMO group increased significantly, leucine and cysteine concentration were both increased significantly (P 0.05). Lactate dehydrogenase activity and urea nitrogen concentration in amniotic fluid of proline group and DFMO group were significantly increased, while arginine concentration was significantly decreased (P 0.05); urea nitrogen, uric acid and creatinine concentration in allantoic fluid of all treatment groups were significantly increased (P 0.05), glutamate and arginine concentration in proline group and DFMO group were significantly decreased (P 0.05). There was no significant difference in plasma * * * chemical composition between piglets and piglets in Huanjiang piglets (P0.05). The plasma ammonia concentration in proline group and DFMO group was significantly lower than that in alanine group at 7 days old (P0.05); at 14 days of age, the weight of piglets in proline group was significantly higher than that in other two groups (P0.05). The plasma glucose, arginine and proline concentrations in the DFMO group were significantly higher than those in the proline group (P 0.05), and the plasma ammonia and glucose concentrations in the DFMO group were significantly lower than those in the proline group (P 0.05), the concentrations of arginine, ornithine and asparagine were significantly higher than those in the alanine group (P 0.05), and the concentration of asparagine was significantly higher than that in the proline group (P 0.05). The fetal weight of the minimum weight group was significantly lower than that of the maximum weight group and the middle weight group (P 0.05) on the seventieth day of gestation. On the 45th day of gestation, the fetal weight of the minimum weight group was significantly lower than that of the maximum weight and the middle weight group. Concentrations of glucose and triglyceride were significantly lower than those of the intermediate weight group (P 0.05), the concentration of arginine in amniotic fluid corresponding to the maximum weight group was significantly higher than that of the intermediate weight group (P 0.05), and the concentration of lactic acid in allantoic fluid was significantly lower than that of the intermediate weight group (P 0.05). (P 0.05), the concentrations of amniotic fluid creatinine and triglyceride in the minimal weight group were significantly higher than those in the maximum weight group (P 0.05). The contents of some nutrients and their metabolites in amniotic fluid and allantoic fluid are closely related.
【学位授予单位】：江西农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S828.5
本文编号：2210399
[Abstract]:In order to investigate the effects of dietary proline supplementation on reproductive performance and offspring growth of Huanjiang Xiang pig, 48 * * * Huanjiang sow sows with a weight of about 25 Kg were selected. They were naturally copulatory and 15 d after mating. They were randomly divided into 3 groups, each containing 8 replicates (columns) and 2 pigs per replicate. Feeding 0.77%L- alanine (equal nitrogen contrast), 1, respectively. 0% L-proline, 0.77% L-alanine + 0.0167% difluoromethyl ornithine (DFMO, polyamine synthesis inhibitor) test diet. At the 45th and 70th day of mating, one sow in each column was selected, bled and killed, blood was collected, plasma was separated for biochemical parameters, free amino acids and polyamines analysis; dissected sows, removed the whole uterus, and separated and weighed pregnancy weight. The number of corpus luteum was recorded, the amniotic fluid and allantoic fluid were collected, the * * * the fetal pig was stripped one by one, the weight of the fetal pig was recorded and the number of fetuses was recorded; the alanine group was selected with 1 largest, near average and minimum weight fetal water and amniotic fluid respectively, and the other 2 groups of 1 sows and the allantoic fluid which were close to the average weight of the pigs were used for biochemical analysis. Analysis of parameters and free amino acids. In addition, 15 Huanjiang sows with a weight of about 25 Kg were selected and naturally bred * * *. After mating 15 d, they were randomly divided into 3 groups. Each group had 5 replicates (columns) and 1 pigs per replicate. The diet was treated with the same method. The number of piglets and the weight of piglets were recorded in 2 h after natural childbirth, and the largest, near average and minimum weight piglets were used for other purposes. To study the effect of proline on intestinal function and development of newborn piglets, the remaining piglets were fed by suckling sows. One piglet was randomly selected at the age of 7 and 14 days. After weighing, one piglet was killed. Blood was collected and plasma was separated for biochemical parameters, free amino acids and polyamines analysis. The results showed that: 1. The effect of proline supplementation on reproductive performance and plasma chemical composition of pregnant Huanjiang Xiang pig. The litter weight of proline group was significantly higher than that of alanine group on the seventieth day of pregnancy (P 0.05). The number of corpus luteum, fetal number, litter weight, pregnancy weight and fetal weight of other experimental groups were not significantly different (P 0.05). The plasma glutamate-alanine transaminase activity and lactate concentration in proline group were significantly lower than those in alanine group (P Amide concentration decreased significantly, 3-methylhistidine concentration increased significantly (P 0.05). 2. Effects of dietary proline supplementation on amniotic fluid and allantoic liquefaction chemical composition of pregnant Huanjiang Xiang pig. Fetal weight and gestational weight of each treatment group had no significant difference on the 45th and 70th days of pregnancy (P 0.05). At the 45th day of pregnancy, aspartic acid, glutamate and isoleucine in amniotic fluid of DFMO group were not significantly different. The concentrations of acid and arginine were significantly higher than those of alanine group (P 0.05); the concentrations of amniotic fluid cysteine, allantoic albumin and arginine in proline group were significantly higher than those in alanine group on the seventieth day of pregnancy (P 0.05); the other indicators were not significantly different between groups (P 0.05). Compared with the seventieth and forty-five days of pregnancy, the amniotic fluid oxaloacetic acid transamination in each treatment group was significantly higher. Amniotic fluid gamma-glutamyl transpeptidase activity and lysine concentration in alanine group and proline group increased significantly (P 0.05). Amniotic fluid glucose concentration in alanine group and DFMO group increased significantly, leucine and cysteine concentration were both increased significantly (P 0.05). Lactate dehydrogenase activity and urea nitrogen concentration in amniotic fluid of proline group and DFMO group were significantly increased, while arginine concentration was significantly decreased (P 0.05); urea nitrogen, uric acid and creatinine concentration in allantoic fluid of all treatment groups were significantly increased (P 0.05), glutamate and arginine concentration in proline group and DFMO group were significantly decreased (P 0.05). There was no significant difference in plasma * * * chemical composition between piglets and piglets in Huanjiang piglets (P0.05). The plasma ammonia concentration in proline group and DFMO group was significantly lower than that in alanine group at 7 days old (P0.05); at 14 days of age, the weight of piglets in proline group was significantly higher than that in other two groups (P0.05). The plasma glucose, arginine and proline concentrations in the DFMO group were significantly higher than those in the proline group (P 0.05), and the plasma ammonia and glucose concentrations in the DFMO group were significantly lower than those in the proline group (P 0.05), the concentrations of arginine, ornithine and asparagine were significantly higher than those in the alanine group (P 0.05), and the concentration of asparagine was significantly higher than that in the proline group (P 0.05). The fetal weight of the minimum weight group was significantly lower than that of the maximum weight group and the middle weight group (P 0.05) on the seventieth day of gestation. On the 45th day of gestation, the fetal weight of the minimum weight group was significantly lower than that of the maximum weight and the middle weight group. Concentrations of glucose and triglyceride were significantly lower than those of the intermediate weight group (P 0.05), the concentration of arginine in amniotic fluid corresponding to the maximum weight group was significantly higher than that of the intermediate weight group (P 0.05), and the concentration of lactic acid in allantoic fluid was significantly lower than that of the intermediate weight group (P 0.05). (P 0.05), the concentrations of amniotic fluid creatinine and triglyceride in the minimal weight group were significantly higher than those in the maximum weight group (P 0.05). The contents of some nutrients and their metabolites in amniotic fluid and allantoic fluid are closely related.
【学位授予单位】：江西农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S828.5
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