延边黄牛ANGPTL4基因组织表达规律与肌内脂肪沉积相关性研究

发布时间：2018-09-01 14:25

【摘要】：为分析ANGPTL4基因在延边黄牛各组织中的表达差异,进一步探讨该基因在肌内脂肪沉积中的作用,本实验利用q RT-PCR法比较分析了ANGPTL4基因在延边黄牛7个组织(心、肝、脾、肺、肾、背最长肌和后腿肌)中的表达水平及其与肌内脂肪沉积的相关性。结果表明:ANGPTL4基因在7个组织中表达水平从高到低依次为肝、肺、肾、心、脾、背最长肌和后腿肌,肝中表达量极显著高于心、脾、肺、肾、后腿肌和背最长肌组织(P0.01),肺中表达量显著高于心、脾、肾、后腿肌和背最长肌组织(P0.05);背最长肌中ANGPTL4基因的表达量与背最长肌的IMF含量呈显著正相关(P0.05),相关系数为0.924;ANGPTL4基因在后腿肌的表达量与后腿肌的IMF含量呈显著正相关(P0.05),相关系数为0.998。结果显示,ANGPTL4基因影响延边黄牛肌内脂肪沉积。
[Abstract]:In order to analyze the difference of expression of ANGPTL4 gene in the tissues of Yanbian cattle, and to explore the role of ANGPTL4 gene in intramuscular fat deposition, ANGPTL4 gene was compared in seven tissues (heart, liver, spleen, lung, kidney) of Yanbian cattle by using Q RT-PCR method. The expression level of longissimus dorsi and hind leg muscle and its correlation with intramuscular fat deposition. The results showed that the expression levels of the gene in liver, lung, kidney, heart, spleen, longissimus dorsi and hind leg were significantly higher than those in heart, spleen, lung and kidney. The expression in the lung was significantly higher than that in the heart, spleen and kidney (P0.01) in the hind leg muscle and the longissimus dorsi muscle (P0.01). The expression of ANGPTL4 gene in the longissimus dorsi muscle was positively correlated with the IMF content of the longissimus dorsi muscle (P0.05), the correlation coefficient was 0.924 ANGPTL4 gene expression in the leg muscle and the IMF content in the hind leg muscle (P0.05), the correlation coefficient was 0.998. The results showed that ANGPTL 4 gene affected intramuscular fat deposition in Yanbian cattle.
【作者单位】：延边大学农学院;
【基金】：吉林省科技发展计划重点科技攻关项目(20160204017NY) 吉林省现代肉牛产业技术体系建设研究课题(201635) 吉林省教育厅“十三五”科学技术项目
【分类号】：Q78;S823.81

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