EGF对牦牛冻精运动性能、细胞凋亡和IVF胚胎发育潜力的影响

发布时间：2018-09-05 09:10

【摘要】：本研究旨在探明牦牛冻精体外获能过程中,EGF是否调控精子的细胞凋亡、运动性能及其对后续胚胎发育的影响。在牦牛冻精体外获能过程中,加入不同浓度EGF(0、50、75、100、150和200ng·mL-1),应用精子分析仪分析不同处理组精子活性;运用qRT-RCR、Western blot检测Bax和Bcl-2的表达;评估IVF后受精胚的发育能力。结果表明:1)EGF可显著提高牦牛冻精运动性能、胚胎卵裂率(P0.05),且这种调控具有一定的浓度依赖性。2)75~150ng·mL~(-1) EGF可以显著提高获能冻精Bcl-2基因和蛋白表达,降低Bax表达(P0.05)。综上表明,牦牛冻精体外获能过程中,EGF不仅降低牦牛冷冻精子的细胞凋亡和提高牦牛精子运动性能,而且能够提高IVF后胚胎发育潜力,且其最佳作用浓度为75~150ng·mL~(-1)。本研究为通过生长因子作用提高牦牛体外胚胎生产提供重要参考信息。
[Abstract]:The purpose of this study was to investigate whether EGF regulates the apoptosis of spermatozoa, its motor performance and its effect on subsequent embryo development in the process of yak frozen spermatozoa capacitation in vitro. During the in vitro capacitation of yak frozen spermatozoa, the sperm activity of different treatment groups was analyzed by sperm analyzer, the expression of Bax and Bcl-2 was detected by qRT-RCR,Western blot, and the developmental ability of fertilized embryos after IVF was evaluated by adding different concentrations of EGF (050,75100150 and 200ng mL-1). The results showed that EGF could significantly improve the performance of frozen spermatozoa and the cleavage rate of embryo (P0.05), and this regulation was in a dose-dependent manner. 75~150ng mL~ (-1) EGF could significantly increase the expression of Bcl-2 gene and protein and decrease the expression of Bax (P0.05). The results showed that EGF not only decreased the apoptosis of frozen yak spermatozoa and improved the motility of yak sperm, but also increased the embryo development potential after IVF. The best concentration of EGF was 75~150ng mL~ (-1). This study provides important reference information for enhancing in vitro embryo production of yak by the action of growth factor.
【作者单位】：甘肃农业大学动物医学院甘肃省牛羊胚胎工程技术研究中心;
【基金】：甘肃省农业生物技术研究与应用开发项目(GNSW-2015-12)
【分类号】：S823.85

【参考文献】