狐源犬链球菌的分离鉴定
发布时间：2018-09-13 08:27
【摘要】：对40余份送检狐狸病料进行细菌分离,采用16S rRNA基因测序和生化试验进行鉴定,并进行细菌耐药性和致病性试验。结果显示:在病料中分离到5株革兰阳性球菌,综合鉴定为犬链球菌;该菌对氨苄西林、氯霉素、环丙沙星等药物敏感,但是对四环素有较强的耐药性;小鼠致病性试验显示该菌犬链球菌QL001的半数致死量为5×10~6cfu,有较强的致病性。研究结果为狐狸链球菌病的防控奠定了基础。
[Abstract]:More than 40 samples of fox disease samples were isolated and identified by 16s rRNA gene sequencing and biochemical test. The drug resistance and pathogenicity of the bacteria were tested. The results showed that 5 strains of Gram-positive cocci were isolated and identified as Streptococcus canis, which was sensitive to ampicillin, chloramphenicol and ciprofloxacin, but resistant to tetracycline. Mouse pathogenicity test showed that the median lethal dose of Streptococcus canis QL001 was 5 脳 10 ~ (6) cfu. it had strong pathogenicity. The results laid a foundation for the prevention and control of fox streptococcus disease.
【作者单位】： 东北林业大学野生动物资源学院;
【基金】：国家自然科学基金(31502119)
【分类号】：S858.92
，
本文编号：2240604
[Abstract]:More than 40 samples of fox disease samples were isolated and identified by 16s rRNA gene sequencing and biochemical test. The drug resistance and pathogenicity of the bacteria were tested. The results showed that 5 strains of Gram-positive cocci were isolated and identified as Streptococcus canis, which was sensitive to ampicillin, chloramphenicol and ciprofloxacin, but resistant to tetracycline. Mouse pathogenicity test showed that the median lethal dose of Streptococcus canis QL001 was 5 脳 10 ~ (6) cfu. it had strong pathogenicity. The results laid a foundation for the prevention and control of fox streptococcus disease.
【作者单位】： 东北林业大学野生动物资源学院;
【基金】：国家自然科学基金(31502119)
【分类号】：S858.92
，
本文编号：2240604
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