高通量测序分析酵母培养物对肉仔鸡盲肠菌群的影响

发布时间：2018-09-13 08:32

【摘要】：本实验旨在探讨酵母培养物对肉仔鸡盲肠菌群的影响。选取336羽健康且体重相近的1日龄AA肉仔鸡,随机分7个处理组,每组6个重复,每个重复8只。A组(对照组)饲喂基础日粮,B、C、D、E、F组分别在基础日粮中添加发酵时间为12、24、36、48、60 h的酵母培养物,G组在基础日粮中添加"达农威益康V XP"酵母培养物产品。预试期7 d,正试期42 d。采用Illumina Miseq高通量测序技术对肉仔鸡盲肠内容物菌群分析。结果表明:与A组(对照组)相比,C、D组肉仔鸡的平均日增重显著提高(P0.05),耗料增重比显著降低(P0.05);而D组肉仔鸡盲肠菌群物种多样性最高,C组最低;在Phylum(门)的水平上,各组中的Firmicutes(厚壁菌门)比例最高,而C组样品中Verrucomicrobia(疣微菌门)含量较其他组显著增加;在Genus(属)的水平上,7组样品的盲肠内容物中以Bacteroides、Faecalibacterium、Lactobacillus、Blautia等菌属为主。综上,不同发酵时间的酵母培养物对肠道菌群有显著影响,可通过调整发酵时间来控制其代谢产物,进而影响动物的生产性能。
[Abstract]:The purpose of this study was to investigate the effect of yeast culture on the cecum flora of broilers. A total of 336healthy 1-day-old AA broilers with similar body weight were randomly divided into 7 treatment groups with 6 replicates in each group. Each group (control group) was fed with yeast culture (group G) supplemented with "Danongwei Yikang V XP" yeast culture product in the basal diet with fermentation time of 12 ~ (24) ~ 36 ~ (6) and 48 ~ (60) h respectively in the basal diet of the control group (n = 8), and the control group was fed with "Danongwei Yikang V XP" yeast culture product in the basal diet. The pretest period was 7 days and the positive test period was 42 days. Illumina Miseq high throughput sequencing technique was used to analyze the bacterial flora of cecum contents in broilers. The results showed that compared with group A (control group), the average daily gain (ADG) increased significantly (P0.05) and the feed gain ratio decreased significantly (P0.05), while the highest species diversity of cecum flora in group D was the lowest in group C (P 0.05), and at the level of Phylum (gate), the average daily gain of broilers was significantly higher than that of control group (P 0.05), while that of group D was significantly lower than that of group C (P 0.05). The proportion of Firmicutes in each group was the highest, while the content of Verrucomicrobia in group C was significantly higher than that in other groups, and Bacteroides,Faecalibacterium,Lactobacillus,Blautia was the dominant bacteria in the contents of cecum in group C at the level of Genus (genus). In conclusion, yeast cultures with different fermentation time have a significant effect on intestinal flora. The metabolic products can be controlled by adjusting fermentation time, and then the production performance of animals can be affected.
【作者单位】：吉林农业大学生命科学学院;长春博瑞饲料集团有限公司技术中心;吉林农业大学动物科技学院;
【基金】：吉林省教育厅“十二五”科学技术研究项目(2015181))
【分类号】：S831.5

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