陕北白绒山羊种公羊不同采精频率下能量需要量研究
发布时间：2018-09-14 08:55
【摘要】：本研究以陕北白绒山羊种公羊为研究对象,采用饲养试验和消化试验相结合的研究方法,研究了不同能量摄入水平对绒山羊种公羊日增重、养分消化率、血清生化指标和精液品质的影响,进而分别对种公羊预备期、正式配种期和恢复期能量需要量进行了研究,旨在为建立和完善陕北白绒山羊饲养标准提供基础参数。本研究选取12只体重相近(54.95±2.21 kg)、体况良好、精液品质相近的陕北白绒山羊成年种公羊,按照随机区组设计分为4组,每组3个重复,每个重复1只羊,分别饲喂4种日粮。日粮按NRC(1981,2007)标准推荐量的85%(5.95 MJ/kg),100%(7.00MJ/kg),115%(8.05 MJ/kg)和130%(9.10 MJ/kg)设置4个能量梯度。试验期120 d,分为配种预备期(50 d)、配种期(40 d)和恢复期(30 d)三个阶段。每个试验阶段最后进行10 d的消化试验,消化试验采用外源指示剂法进行,指示剂为Cr2O3。结果如下:(1)不同能量摄入水平对试验公羊各时期的平均日增重(ADG)均产生了极显著影响(P0.01);5.95 MJ/kg组和7.00 MJ/kg组试验公羊在整个试验期体重先减后增,8.05MJ/kg组和9.10 MJ/kg组公羊体重呈现先增后减再增的趋势。(2)在本试验条件下,试验公羊的总能(GE)和干物质(DM)消化率随日粮能量摄入水平的升高而显著升高(P0.05)。在预备期和配种期,日粮能量摄入水平对试验公羊的粗蛋白(CP)消化率影响显著(P0.05),对公羊的中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)的消化率没有产生显著影响(P0.05),但随着能量摄入水平的提高呈现增高的趋势;在恢复期,能量摄入水平对试验公羊的CP和ADF的消化率没有显著影响,但对试验公羊的NDF消化率产生了显著影响(P0.05)。(3)能量摄入水平对试验公羊各时期的谷丙转氨酶(GPT)和谷草转氨酶(GOT)的活性没有显著影响。随着能量摄入水平的提高,试验公羊的葡萄糖(GLU)、甘油三酯(TG)和总胆固醇(TC)浓度呈上升趋势,血清尿素氮(UN)呈下降趋势。(4)在整个配种期,采精频率对试验公羊的睾酮浓度和射精量产生了极显著影响(P0.01)。日粮能量水平对试验公羊的射精量和精子密度显著(P0.05),对有效精子数影响极显著(P0.01),对精子活力影响不显著(P0.05)。射精量、精子密度和有效精子数均以8.05 MJ/kg组最好。5天2次采精频率下试验公羊的精液品质优于5天4次的采精频率。采精频率和能量对试验公羊的血清睾酮浓度产生了极显著的交互作用(P0.01),对射精量、精子活力、精子密度和有效精子数的交互作用不显著(P0.05)。(5)陕北白绒山羊种公羊的消化能(DE)和代谢能(ME)的回归公式分别为:预备期:DE(MJ/d)= 0.642W0.75+ 0.083ADG(R2=0.993,P=0.002)ME(MJ/d)= 0.527W0.75+ 0.068ADG(R2=0.993,P=0.002);配种期:DE(MJ/d)=(0.642+0.4644n)W0.75,(n为采精次数/d)ME(MJ/d)=(0.527+0.3800n)W0.75;恢复期DE(MJ/d)= 0.555W0.75 + 0.064ADG(R2=0.996,P=0.001)ME(MJ/d)= 0.455W0.75+ 0.053ADG(R2=0.996,P=0.001)。(6)本试验推荐陕北白绒山羊种公羊配种期日粮的适宜ME浓度范围为8.05 MJ/kg~8.99 MJ/kg。
[Abstract]:In this study, we studied the effect of different levels of energy intake on daily gain and nutrient digestibility of white cashmere goat breeds in Northern Shaanxi Province by combining feeding experiment with digestibility test, and the effects of different energy intake levels on daily gain and nutrient digestibility of cashmere goats were studied. The effects of serum biochemical indexes and semen quality on the energy requirements in preparation period, formal breeding period and convalescence stage of male sheep were studied in order to provide basic parameters for establishing and perfecting the raising standard of Northern Shaanxi White Cashmere Goat. In this study, 12 adult male goats with similar body weight (54.95 卤2.21 kg),) and similar semen quality were divided into 4 groups according to random block design. Dietary energy gradients were set at 85% (5.95 MJ/kg), 100% (7.00MJ/kg), 115% (8.05 MJ/kg) and 130% (9.10 MJ/kg) of NRC (1981 ~ 2007). The experiment period was 120 days, which was divided into three stages: mating preparation period (50 d),) mating period (40 d) and convalescence stage (30 d). At the end of each trial stage, the digestion test was carried out for 10 days. The digestion test was carried out by exogenous indicator method with Cr2O3. as the indicator. The results were as follows: (1) different levels of energy intake had a significant effect on the average daily gain (ADG) of the experimental ram in each period (P0.01). (P0.01) the weight loss and gain of 8.05 MJ / kg group and 9.10 MJ/kg group of male sheep in 5.95 MJ/kg group and 7.00 MJ/kg group during the whole trial period were very significant (P0.01). There is a tendency to increase first and then decrease then increase. (2) under this experimental condition, The digestibility of total energy (GE) and dry matter (DM) increased with the increase of dietary energy intake (P0.05). During the preparatory period and the mating period, Dietary energy intake had significant effect on the digestibility of crude protein (CP) (P0.05), but had no significant effect on the digestibility of neutral detergent fiber (NDF) and acidic detergent fiber (ADF) in male sheep (P0.05), but with the increase of energy intake level. Showing an upward trend; During the recovery period, the levels of energy intake had no significant effect on the digestibility of CP and ADF in the experimental ram. However, the NDF digestibility of the male sheep was significantly affected (P0.05). (3). The level of energy intake had no significant effect on the activities of (GPT) and (GOT) of glutamic pyruvic transaminase (GPT) and alanine aminotransferase (GOT). With the increase of energy intake, the concentrations of glucose (GLU), triglyceride (TG) and total cholesterol (TC) increased, and the serum urea nitrogen (UN) decreased. The frequency of ejaculation had a significant effect on testosterone concentration and ejaculation (P0.01). Dietary energy levels had significant effects on ejaculation and sperm density (P0.05), on effective sperm count (P0.01), and on sperm motility (P0.05). The ejaculation quantity, sperm density and effective sperm number were 8. 05 MJ/kg. The semen quality of male sheep was better than that of 4 ejaculation times in 5 days. The frequency and energy of spermatozoa had a significant interaction on serum testosterone concentration (P0.01), and on ejaculation and sperm motility. 绮惧瓙瀵嗗害鍜屾湁鏁堢簿瀛愭暟鐨勪氦浜掍綔鐢ㄤ笉鏄捐憲(P0.05).(5)闄曞寳鐧界粧灞辩緤绉嶅叕缇婄殑娑堝寲鑳，
本文编号：2242204
[Abstract]:In this study, we studied the effect of different levels of energy intake on daily gain and nutrient digestibility of white cashmere goat breeds in Northern Shaanxi Province by combining feeding experiment with digestibility test, and the effects of different energy intake levels on daily gain and nutrient digestibility of cashmere goats were studied. The effects of serum biochemical indexes and semen quality on the energy requirements in preparation period, formal breeding period and convalescence stage of male sheep were studied in order to provide basic parameters for establishing and perfecting the raising standard of Northern Shaanxi White Cashmere Goat. In this study, 12 adult male goats with similar body weight (54.95 卤2.21 kg),) and similar semen quality were divided into 4 groups according to random block design. Dietary energy gradients were set at 85% (5.95 MJ/kg), 100% (7.00MJ/kg), 115% (8.05 MJ/kg) and 130% (9.10 MJ/kg) of NRC (1981 ~ 2007). The experiment period was 120 days, which was divided into three stages: mating preparation period (50 d),) mating period (40 d) and convalescence stage (30 d). At the end of each trial stage, the digestion test was carried out for 10 days. The digestion test was carried out by exogenous indicator method with Cr2O3. as the indicator. The results were as follows: (1) different levels of energy intake had a significant effect on the average daily gain (ADG) of the experimental ram in each period (P0.01). (P0.01) the weight loss and gain of 8.05 MJ / kg group and 9.10 MJ/kg group of male sheep in 5.95 MJ/kg group and 7.00 MJ/kg group during the whole trial period were very significant (P0.01). There is a tendency to increase first and then decrease then increase. (2) under this experimental condition, The digestibility of total energy (GE) and dry matter (DM) increased with the increase of dietary energy intake (P0.05). During the preparatory period and the mating period, Dietary energy intake had significant effect on the digestibility of crude protein (CP) (P0.05), but had no significant effect on the digestibility of neutral detergent fiber (NDF) and acidic detergent fiber (ADF) in male sheep (P0.05), but with the increase of energy intake level. Showing an upward trend; During the recovery period, the levels of energy intake had no significant effect on the digestibility of CP and ADF in the experimental ram. However, the NDF digestibility of the male sheep was significantly affected (P0.05). (3). The level of energy intake had no significant effect on the activities of (GPT) and (GOT) of glutamic pyruvic transaminase (GPT) and alanine aminotransferase (GOT). With the increase of energy intake, the concentrations of glucose (GLU), triglyceride (TG) and total cholesterol (TC) increased, and the serum urea nitrogen (UN) decreased. The frequency of ejaculation had a significant effect on testosterone concentration and ejaculation (P0.01). Dietary energy levels had significant effects on ejaculation and sperm density (P0.05), on effective sperm count (P0.01), and on sperm motility (P0.05). The ejaculation quantity, sperm density and effective sperm number were 8. 05 MJ/kg. The semen quality of male sheep was better than that of 4 ejaculation times in 5 days. The frequency and energy of spermatozoa had a significant interaction on serum testosterone concentration (P0.01), and on ejaculation and sperm motility. 绮惧瓙瀵嗗害鍜屾湁鏁堢簿瀛愭暟鐨勪氦浜掍綔鐢ㄤ笉鏄捐憲(P0.05).(5)闄曞寳鐧界粧灞辩緤绉嶅叕缇婄殑娑堝寲鑳，
本文编号：2242204
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