河北省4个黑猪群体的遗传多样性分析

发布时间：2018-09-17 14:47

【摘要】：为合理利用地方猪遗传资源,随机选取河北省4个市的4个黑猪群体,采用FAO-ISAG联合推荐的8个微卫星标记对各样本群体的等位基因数、有效等位基因数、杂合度、多态信息含量及F-统计量进行检测。结果显示,8个微卫星座位共检测到66个等位基因。有效等位基因数(3.103~5.349),观察杂合度(0.346~0.437),期望杂合度(0.638~0.790)和多态信息含量(0.578~0.762)4个指标,表明4个猪群体具有丰富的多样性。所检测的微卫星位点均偏离平衡状态,主要是由于起始群体规模小或公猪家系少所致。F统计量结果表明,4个猪群体都存在一定程度的近交。依据对4个猪群的分析结果,群体1、2可通过扩大血统数,以减缓近交程度;群体3、4应开展选育,以提高群体一致性。
[Abstract]:In order to make rational use of local pig genetic resources, four black pig populations from 4 cities of Hebei Province were randomly selected. The alleles, effective alleles and heterozygosity of each sample population were determined by using 8 microsatellite markers recommended by FAO-ISAG. Polymorphic information content and F-statistic were detected. The results showed that 66 alleles were detected in 8 microsatellite loci. The number of effective alleles (3.103 ~ 5.349), the heterozygosity (0.346 ~ 0.437), the expected heterozygosity (0.638 ~ 0.790) and the polymorphic information content (0.578 ~ 0.762) were observed, which indicated that the four pig populations had rich diversity. The microsatellite loci were all deviated from the equilibrium state, which was mainly due to the small size of the initial population or the small family size of the boar. The results of .F statistics showed that all the four pig populations had inbreeding to some extent. According to the results of the analysis of 4 breeds, the population 1 / 2 could be expanded to reduce the inbreeding degree, and the breeding of population 3Y4 should be carried out in order to improve the consistency of the population.
【作者单位】：河北农业大学动物科技学院;国家北方山区农业工程技术研究中心;河北省山区农业工程技术研究中心;河北省辛集市正农牧业有限公司;河北省畜牧兽医研究所;易县畜牧站;
【基金】：河北省科技计划项目(14236602D)
【分类号】：S828

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