关中奶山羊PIS区域序列多态性分析

发布时间：2018-10-07 19:03

【摘要】：山羊无角性状是畜牧业生产中重要的经济性状,报道显示山羊无角间性综合征(polled intersex syndrome,PIS)控制着山羊的无角和间性性状。山羊PIS序列定位于1号染色体约11.7kb的序列上,试验旨在研究这段序列对关中奶山羊(3只有角山羊、3只无角山羊)无角性状的影响。选用4对引物(PIS1-1、PIS1-2、PIS2、PIS3)扩增关中奶山羊的PIS序列,第5对引物(PIS whole)用于检测PIS序列的完全缺失,对测序结果进行拼接、多态性分析,并对序列进行基因注释。结果发现,试验成功扩增出山羊PIS的全长序列(12.814kb),BLAST比对结果正确。PIS whole引物没有扩增出任何条带,说明关中奶山羊中不存在11.7kb的完全缺失。经SeqMan软件分析拼接序列发现30个多态性位点,这些多态性位点可能与关中奶山羊有角/无角性状相关。对拼接序列进行基因注释,发现存在1个tRNA编码位点、2个微卫星、3个microRNA编码位点及L1-EN、RT-nLTR-like样保守结构域,并发现1个CpG岛、1对反向重复序列和2段串联重复序列。本试验结果对关中奶山羊无角性状的分子机理研究具有重要的参考价值。
[Abstract]:Goat hornless trait is an important economic trait in animal husbandry. It is reported that goat acerneless syndrome (polled intersex syndrome,PIS) controls the anguless and intersex traits of goat. The PIS sequence of goat was located on the approximate 11.7kb sequence of chromosome 1. The purpose of the experiment was to study the effect of this sequence on the hornless traits of Guanzhong dairy goats (3 horned goats and 3 hornless goats). Four pairs of primers (PIS1-1,PIS1-2,PIS2,PIS3) were used to amplify the PIS sequence of Guanzhong dairy goat. The fifth pair of primers (PIS whole) was used to detect the complete deletion of PIS sequence. The results showed that the full-length sequence (12.814kb) of goat PIS (12.814kb) blast was successfully amplified. The results showed that there were no bands amplified by the primers of PiS whole, which indicated that there was no complete deletion of 11.7kb in Guanzhong dairy goat. Thirty polymorphic loci were found by SeqMan software, which may be associated with horned / anguless traits in Guanzhong dairy goats. Gene annotation showed that there were 1 tRNA coding site, 2 microsatellites, 3 microRNA coding sites and L1-ENN RT-nLTR-like conserved domain, and 1 CpG island 1 pair of reverse repeats and 2 tandem repeats. The results of this study have important reference value for studying the molecular mechanism of hornless traits in Guanzhong dairy goats.
【作者单位】：广西大学亚热带生物资源保护与利用国家重点实验室;
【基金】：国家863重点项目课题(2013AA102504) 广西科技开发项目桂科攻(1598013-2)
【分类号】：S827

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