林麝肺炎克雷伯氏菌的分离鉴定及其kp05372基因的生物信息学分析

发布时间：2018-10-07 20:23

【摘要】：【目的】对1株疑似肺炎克雷伯氏菌进行分离鉴定及全基因测序分析,并对发现的新基因kp05372编码产物进行生物信息学分析。【方法】采用传统的细菌鉴定方法和分子生物学方法,分别对采自麝粪便的1株病原菌的生理生化特征及16SrRNA序列进行测定;以小鼠感染试验和半数致死量(LD50)测试该病原菌的致病性;用全基因组测序方法对病原菌基因组进行测序分析,并对发现的新基因编码产物进行生物信息学分析。【结果】经生理生化试验和16SrRNA序列分析发现,分离菌株为肺炎克雷伯氏菌,命名为GPKP,其对小鼠致死的LD50为6.3×107CFU/mL。该菌基因组大小为4 879 707bp,包含5 490个开放阅读框,共注释到22组COG功能。kp05372基因编码产物由302个氨基酸组成,无信号肽,是一种不稳定的、在细胞内发挥生理作用的亲水性蛋白。【结论】分离的细菌为肺炎克雷伯氏菌,kp05372基因编码产物属于典型的LysR家族转录因子(LTTR)。
[Abstract]:[objective] to isolate and identify a suspected Klebsiella pneumoniae strain and complete gene sequencing. The bioinformatics analysis of the novel gene kp05372 coding product was carried out. [methods] the physiological and biochemical characteristics and 16SrRNA sequence of a pathogen collected from faeces of musk deer were determined by traditional methods of bacterial identification and molecular biology. The pathogenicity of the pathogen was tested by mouse infection test and half lethal dose (LD50). The new gene encoding product was analyzed by bioinformatics. [results] the isolated strain was Klebsiella pneumoniae by physiological and biochemical tests and 16SrRNA sequence analysis. The isolated strain was named Klebsiella pneumoniae and its lethal LD50 to mice was 6.3 脳 10 ~ (7) CFU / mL 路mL ~ (-1). The genome size of the strain is 4 879 707bpand contains 5 490 open reading frames. The results show that 22 groups of COG function. Kp05372 gene encoding products are composed of 302 amino acids and have no signal peptide. [conclusion] the isolated bacterium is Klebsiella pneumoniae kp05372 gene encoding product belongs to the typical LysR family transcription factor (LTTR).
【作者单位】：四川农业大学动物医学院;四川养麝研究所;
【基金】：四川省学术和技术带头人培养资助项目四川农业大学双支项目
【分类号】：S852.61

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