气肿疽梭菌胶体金诊断试纸条的研制
发布时间：2018-10-08 12:12
【摘要】：为建立快速、准确、简便的检测气肿疽的胶体金免疫层析试纸条,以免疫层析法为指导,根据抗原-抗体的特性,建立了不需要再加入任何检测试剂且快速简便的检测方法。结果表明,胶体金与该单抗的最佳结合量为30μg/m L,最佳pH值为8.4,最佳NC膜为Millipore HFB1350220型。将纯化的气肿疽梭菌多抗及羊抗鼠IgG分别以1∶4和1∶8的稀释倍数喷于硝酸纤维素膜的T线和C线。对胶体金诊断试纸条进行验证,敏感度高,可检测到的最低抗原量为1×10~5CFU/m L,特异性强、无假阳性与交叉反应现象、有较好的稳定性。本研究旨在建立快速、准确、简便的检测气肿疽的胶体金免疫层析试纸条。
[Abstract]:In order to establish a rapid, accurate and simple gold immunochromatographic strip for the detection of emphysema, a rapid and simple method was established based on the characteristics of antigen-antibody and immunochromatographic method. The results show that the best binding amount of colloidal gold to the McAb is 30 渭 g / mL, the optimum pH value is 8.4, and the best NC film is Millipore HFB1350220 type. Purified Clostridium emphysema polyclonal antibody and sheep anti mouse IgG were sprayed on the T line and C line of cellulose nitrate membrane at 1:4 and 1:8 dilution times, respectively. The test strip of colloidal gold diagnosis was proved to be sensitive, the lowest antigen was 1 脳 10~5CFU/m L, the specificity was strong, there was no false positive and cross reaction phenomenon, and there was good stability. The aim of this study was to establish a rapid, accurate and simple gold immunochromatographic strip for the detection of emphysema.
【作者单位】： 延边州畜牧总站/延边州动物疫病预防控制中心/延边大学农学院动物医学系;大理大学公共卫生医学院;
【基金】：吉林省教育厅“十二五”科学技术研究项目(编号:吉教科合字[2014]第6号)
【分类号】：S855.12
[Abstract]:In order to establish a rapid, accurate and simple gold immunochromatographic strip for the detection of emphysema, a rapid and simple method was established based on the characteristics of antigen-antibody and immunochromatographic method. The results show that the best binding amount of colloidal gold to the McAb is 30 渭 g / mL, the optimum pH value is 8.4, and the best NC film is Millipore HFB1350220 type. Purified Clostridium emphysema polyclonal antibody and sheep anti mouse IgG were sprayed on the T line and C line of cellulose nitrate membrane at 1:4 and 1:8 dilution times, respectively. The test strip of colloidal gold diagnosis was proved to be sensitive, the lowest antigen was 1 脳 10~5CFU/m L, the specificity was strong, there was no false positive and cross reaction phenomenon, and there was good stability. The aim of this study was to establish a rapid, accurate and simple gold immunochromatographic strip for the detection of emphysema.
【作者单位】： 延边州畜牧总站/延边州动物疫病预防控制中心/延边大学农学院动物医学系;大理大学公共卫生医学院;
【基金】：吉林省教育厅“十二五”科学技术研究项目(编号:吉教科合字[2014]第6号)
【分类号】：S855.12
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