一株多杀性巴氏杆菌的分离鉴定与耐药性研究

发布时间：2018-10-13 16:54

【摘要】：从扬州大学动物医院收集送检病鸡的病料,无菌采集病死鸡的肝脏、肺脏等组织接种于麦康凯平板和血琼脂平板上进行细菌分离与培养,并对分离菌进行生化试验、16 S r RNA基因序列分析和荚膜分型PCR鉴定,同时用12种抗菌药物对分离菌株进行药敏试验。细菌分离结果显示,分离菌在麦康凯平板上未见菌落生长,在血琼脂平板上呈光滑、湿润的露滴样小菌落;革兰氏染色镜检结果为革兰氏阴性小杆菌,瑞氏染色镜检可见大量两极浓染的短杆菌;生化试验结果表明分离菌株能够发酵葡萄糖、果糖、蔗糖等糖类,不能发酵乳糖和麦芽糖;同时,16 S r RNA基因序列和荚膜分型PCR测定结果显示分离菌为荚膜A型多杀性巴氏杆菌。药敏试验结果表明,分离菌株对痢特灵、左氟沙星、强力霉素的敏感性较高,而对氯霉素、美洛西林、卡那霉素等高度耐药。
[Abstract]:Samples of diseased chickens were collected from Yangzhou University Animal Hospital. The liver and lungs of dead chickens were collected and inoculated with wheat Kang Kai plate and blood Agar plate for bacterial isolation and culture. Biochemistry test, 16 S r RNA gene sequence analysis and PCR identification of capsule typing were carried out on the isolated bacteria. At the same time, 12 antimicrobial agents were used to test the drug sensitivity of the isolated strains. The results of bacterial isolation showed that there was no colony growth on the plate of wheat Kang Kai, but a smooth, moist, dew like colony on the plate of blood Agar, and Gram-negative microbacillus was detected by microscopical staining. The results of biochemical tests showed that the isolated strains could ferment glucose, fructose, sucrose and other sugars, but could not ferment lactose and maltose. At the same time, 16 S r RNA gene sequence and PCR analysis showed that the isolated strain was Pasteurella multocida. The results of drug sensitivity test showed that the isolates were highly sensitive to trifluridine, levofloxacin and doxycycline, but highly resistant to chloramphenicol, meloxicillin and kanamycin.
【作者单位】：扬州大学兽医学院;
【基金】：国家自然科学基金项目(31172300) 江苏省高校自然科学重大项目(12KJA230002) 高等学校博士学科点专项科研基金(博导类)(20133250110002) 江苏高校优势学科建设工程资助项目(PAPD)
【分类号】：S852.61

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