低淀粉饲粮条件下不同瘤胃降解淀粉水平对体外瘤胃发酵的影响

发布时间：2018-10-16 21:12

【摘要】：本试验旨在研究玉米作为淀粉来源的低淀粉饲粮条件下不同瘤胃降解淀粉(RDS)水平对体外瘤胃发酵的影响。以3头安装有永久性瘤胃瘘管的健康荷斯坦奶牛作为瘤胃液供体,各组分别以不同RDS水平饲粮作为发酵底物,体外产气法测定培养48 h时产气量和瘤胃发酵参数以及24 h时瘤胃微生物区系变化。结果表明:1)随着饲粮RDS水平的提高,体外培养48 h时产气量、潜在产气部分和产气速率呈线性升高(P0.05),快速发酵部分产气量呈线性下降(P0.05),干物质消失率呈线性升高(P0.05);2)随着饲粮RDS水平的提高,体外培养48 h时发酵液微生物蛋白、乙酸、丙酸、丁酸和总挥发性脂肪酸浓度呈线性升高(P0.05),pH和氨态氮浓度没有显著变化(P0.05);3)随着饲粮RDS水平的提高,体外培养24 h时发酵液中白色瘤胃球菌和嗜淀粉瘤胃杆菌的相对数量呈线性升高(P0.05),黄色瘤胃球菌、琥珀酸丝状杆菌、溶纤维丁酸弧菌、牛链球菌和溶淀粉琥珀酸单胞菌的相对数量没有显著变化(P0.05)。综合考虑,低淀粉饲粮条件下提高RDS水平有利于瘤胃发酵。
[Abstract]:The purpose of this study was to study the effects of different rumen degradable starch (RDS) levels on rumen fermentation in vitro. Three healthy Holstein cows with permanent rumen fistula were used as rumen fluid donors and each group was fed with different levels of RDS as substrate for fermentation. Gas production and rumen fermentation parameters at 48 h and rumen microflora at 24 h were measured by in vitro gas production method. The results showed that: 1) with the increase of dietary RDS level, gas production was increased after 48 h culture in vitro. The potential gas production and gas production rate increased linearly (P0.05), the gas production of rapid fermentation part decreased linearly (P0.05), the disappearance rate of dry matter increased linearly (P0.05). 2) with the increase of dietary RDS level, microbial protein, acetic acid and propionic acid in fermentation broth were cultured for 48 h in vitro. The concentrations of butyric acid and total volatile fatty acids increased linearly (P0.05), pH and ammonia nitrogen concentration did not change significantly (P0.05); 3) with the increase of dietary RDS level, The relative numbers of Rumus albicans and Rumus eosinophilus in fermentation broth increased linearly after 24 h culture in vitro (P0.05), xanthococcus, filamentous succinate bacilli, Vibrio fibrinolyticus. There was no significant change in the relative number of streptococcus bovis and amylolytic succinomonas (P0.05). Considering comprehensively, the increase of RDS level in low starch diet was beneficial to rumen fermentation.
【作者单位】：东北农业大学动物科学技术学院;
【基金】：国家奶牛产业技术体系项目(CARS-37)
【分类号】：S816

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