基于血清代谢组学研究Kisspeptin对热应激大鼠的保护作用

发布时间：2018-10-17 12:00

【摘要】：运用代谢组学方法研究神经肽Kisspeptin对热应激大鼠的保护作用。本试验通过建立热应激大鼠模型,基于气相色谱-飞行时间质谱联用技术(GC/TOFMS)代谢组学方法检测大鼠血清中内源性代谢物,并采用主成分分析及偏最小二乘判别法分析对照组、热应激组和Kisspeptin干预组之间的代谢物差异,筛选出潜在的生物标志物。代谢组学结果显示热应激组大鼠的血清代谢谱与正常对照组大鼠明显不同,而Kisspeptin干预后大鼠的血清代谢谱有趋于正常的趋势。生化代谢通路分析结果显示热应激引起大鼠三羧酸循环、糖代谢、氨基酸和核苷酸代谢异常,而热应激后给与Kisspeptin处理可缓解上述代谢通路的紊乱并趋于正常状态。此外,氧化应激指标检测结果显示给与Kisspeptin处理可降低热应激大鼠血清中丙二醛(MDA)含量,并提高超氧化酶歧化酶(T-SOD)、总抗氧化能力(T-AOC)和谷胱甘肽过氧化物酶(GSH-Px)的活力,该结果表明Kisspeptin在一定程度上缓解了热应激诱导的氧化应激。该研究从代谢及抗氧化的角度研究了Kisspeptin对热应激大鼠的保护作用,为动物健康养殖和开发抗热应激的安全生物制品提供试验依据。
[Abstract]:The protective effect of neuropeptide Kisspeptin on heat stress rats was studied by metabonomics. In this experiment, the heat stress rat model was established, and the endogenous metabolites in serum were detected by gas chromatography-time-of-flight mass spectrometry (GC/TOFMS), and the control group was analyzed by principal component analysis and partial least-squares discriminant analysis. The differences of metabolites between heat stress group and Kisspeptin intervention group were used to screen out potential biomarkers. The results of metabonomics showed that the serum metabolic spectrum of heat stress group was obviously different from that of normal control group, but the serum metabolic spectrum of Kisspeptin group tended to be normal. The results of biochemical metabolic pathway analysis showed that heat stress induced abnormal metabolism of tricarboxylic acid, glucose, amino acid and nucleotide in rats. However, Kisspeptin treatment after heat stress alleviated the disorder of these metabolic pathways and tended to be normal. In addition, the results of oxidative stress index showed that Kisspeptin treatment could decrease the content of malondialdehyde (MDA) in serum and increase the activities of superoxidase dismutase (T-SOD), total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-Px). The results showed that Kisspeptin alleviated oxidative stress induced by heat stress to some extent. In this study, the protective effects of Kisspeptin on heat stress rats were studied from the point of view of metabolism and antioxidation, which provided experimental basis for healthy breeding of animals and development of safe biological products resistant to heat stress.
【作者单位】：南京农业大学动物医学院;
【分类号】：S852.3

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